Diet crossover study in healthy adults: low vs high glycemic load
211 stool samples from healthy adult participants in a randomized crossover feeding study comparing low-glycemic-load and high-glycemic-load diets, each followed for 28 days with participants serving as their own controls. machine-written summary
211 samples · 211 runs · Age unknown · 2025 · 1.54 Gbp / sample
Checked this project's papers and archive records yourself? Mark it human-verified.
what each source gave
| ✓ Sequence archive | 422 sample values (subject id, timepoint) |
| ✓ Abstract / description | 422 sample values (health condition, health condition detail) |
| – Full text | no open-access full text linked · PMC12489384 |
| ○ Supplementary tables | files read; no table could be matched to the samples |
| – External resources | not covered by the external resources we ingest |
| – User contribution | nothing uploaded yet |
| – Human verified | not yet checked by a person · I checked this project — verify it |
intervention — arms not resolved
Controlled / prescribed diet or feeding regimen design crossover · population healthy_control · High-glycemic load vs low-glycemic load controlled feeding diets, 28 days each, crossover
“randomized cross-over feeding study” — bioproject.description (study level; per-sample arms are in the intervention column below)
longitudinal — not resolved
76 of 77 people sampled more than once (210 samples; median 3, up to 3 per person). Schedule stated by the source: “Stool collected at beginning and end of each 28-day diet period in a crossover design (two diets, 28 days each)” (time origin: intervention start).
Genera, mean relative abundance over 207 profiled gut samples of this project (Sandpiper SingleM profiles, GTDB R232; low-depth and non-metagenome profiles left out).
| field | samples | coverage | R1 | R2 | R3 | R4 |
|---|---|---|---|---|---|---|
| age_category | 211 | 100% | ||||
| country | 0 | 0% | ||||
| health_condition | 211 | 100% | 211 | |||
| subject_id | 211 | 100% | 211 | |||
| health_condition_detail | 211 | 100% | 211 | |||
| timepoint_label | 211 | 100% | 211 |
- Metabolic plasticity of the gut microbiome in response to diets differing in glycemic load in a randomized, crossover, controlled feeding study. 2025 PMID 40619005 PMC12489384 own_data
Archive description
Data is from a completed randomized cross-over feeding study, Carbohydrate and Related Biomarkers (CARB) , where 80 participants consumed two controlled diets for 28 days each. One diet was high in whole grains, fresh fruits and vegetables. The other substituted refined grains for whole grains, and provided carbohydrates from mostly high-glycemic index food sources. All food was provided to participants and energy content and macronutrient composition was identical in both diets. Each participant served as their own control. Participants collected stool at the beginning and end of each diet.
How this project entered the registry
Classified as human gut / stool shotgun DNA metagenome of age unknown by sonnet_x2 with confidence 0.72. Assay shotgun_dna.
- host run.host_tax_id · “9606”
- body site study.description · “Participants collected stool at the beginning”
Evidence examples (one per field × route)
| sample | field | value | route | conf. | source | quote |
|---|---|---|---|---|---|---|
| SAMN42339436 | health_condition | healthy_control | R4 | 0.50 | paper.abstract | healthy men and women |
| SAMN42339436 | health_condition_detail | healthy men and women | R4 | 0.50 | paper.abstract | healthy men and women |
| SAMN42339436 | subject_id | 13620001 | R1 | 0.70 | sample.name_rule:library_name | 13620001_0_DNA |
| SAMN42339436 | timepoint_label | 0 | R1 | 0.70 | sample.name_rule:library_name | 13620001_0_DNA |
Sequencing
211 runs over 211 samples · mean 1.57 Gbp per run (median 1.54 Gbp, total 330.88 Gbp; 211 runs with a base count) · layout PAIRED 211 · platform ILLUMINA 211 · models Illumina HiSeq 2500 (211) · 98% of runs with a Sandpiper profile · first public 2025-06-23
Authors (13)
Hullar MA first · Kahsai O · Curtis KR · Navarro SL · Zhang Y · Randolph TW · Levy L · Shojaie A · Kratz M · Neuhouser ML · Lampe PD · Raftery D · Lampe JW last
Downloads
PRJNA1132291.csv.gz (5.7 KB) · parquet (76.6 KB) — all 211 samples, every field with route and confidence · PRJNA1132291_determinations.csv.gz (5.2 KB) — 844 evidence rows