Inspiration4 astronaut stool metagenome, pre/post spaceflight
8 stool samples from healthy adult crew of the SpaceX Inspiration4 low Earth orbit mission, collected pre-flight (L-92, L-44) and post-flight (R+45, R+82) in a longitudinal design. machine-written summary
8 samples · 8 runs · US · Adult · 2024 · 4.02 Gbp / sample
Checked this project's papers and archive records yourself? Mark it human-verified.
what each source gave
| ✓ Sequence archive | 8 sample values (subject id) |
| ✓ Abstract / description | 40 sample values (collection date, health condition, health condition detail, lifestyle, lifestyle detail) |
| ✓ Full text | 8 sample values (country) · PMC11166662 |
| ○ Supplementary tables | files read; no table could be matched to the samples |
| – External resources | not covered by the external resources we ingest |
| – User contribution | nothing uploaded yet |
| – Human verified | not yet checked by a person · I checked this project — verify it |
longitudinal — not resolved
2 of 2 people sampled more than once (8 samples; median 4, up to 4 per person). Schedule stated by the source: “Stool collected pre-flight (L-92, L-44) and post-flight (R+45, R+82) per subject” (time origin: other: launch/spaceflight date).
Genera, mean relative abundance over 7 profiled gut samples of this project (Sandpiper SingleM profiles, GTDB R232; low-depth and non-metagenome profiles left out).
| field | samples | coverage | R1 | R2 | R3 | R4 |
|---|---|---|---|---|---|---|
| age_category | 8 | 100% | ||||
| country | 8 | 100% | 8 | |||
| health_condition | 8 | 100% | 8 | |||
| subject_id | 8 | 100% | 8 | |||
| collection_date | 8 | 100% | 8 | |||
| health_condition_detail | 8 | 100% | 8 | |||
| lifestyle | 8 | 100% | 8 | |||
| lifestyle_detail | 8 | 100% | 8 |
- Collection of biospecimens from the inspiration4 mission establishes the standards for the space omics and medical atlas (SOMA). 2024 PMID 38862509 PMC11166662 own_data
Archive description
The SpaceX Inspiration4 mission was a 3-day mission with four private astronauts to low Earth orbit that occurred in September 2021. The crew collected biospecimen samples before, during, and after flight. One of these biospecimen collections included stool collected in OMNIgene•GUT tubes (DNA Genotek, OMR-200). Samples were collected pre-flight (L-92, L-44) and post-flight (R+45, R+82). This study hosts stool data. Additional skin, oral and nasal metagenome data is deposited in OSD-572. Dragon capsule data metagenome is deposited in OSD-573. Skin biopsy-site metagenome data is deposited in OSD-574.
How this project entered the registry
Classified as human gut / stool shotgun DNA metagenome of adult by sonnet_x2 with confidence 0.75. Assay shotgun_dna.
- host run.host_tax_id · “9606”
- host run.scientific_name · “human gut metagenome”
- body site study.description · “stool collected in OMNIgene•GUT tubes”
- life stage study.description · “four private astronauts to low Earth orbit”
Evidence examples (one per field × route)
| sample | field | value | route | conf. | source | quote |
|---|---|---|---|---|---|---|
| SAMN42393542 | collection_date | 2021-09 | R4 | 0.45 | ena.study_description | occurred in September 2021 |
| SAMN42393542 | country | US | R3 | 0.70 | paper.body.results | which spanned the US States NY, NJ, TN, and WA |
| SAMN42393542 | health_condition | healthy_control | R4 | 0.35 | study.description | four private astronauts to low Earth orbit |
| SAMN42393542 | health_condition_detail | astronauts selected for spaceflight (presumed healthy) | R4 | 0.35 | study.description | four private astronauts to low Earth orbit |
| SAMN42393542 | lifestyle | spaceflight_or_analog | R4 | 0.45 | ena.study_description | four private astronauts to low Earth orbit |
| SAMN42393542 | lifestyle_detail | Inspiration4 crew | R4 | 0.45 | ena.study_description | four private astronauts to low Earth orbit |
| SAMN42393542 | subject_id | C004 | R1 | 0.70 | sample.name_rule:library_names | C004_stool_R+82 |
Sequencing
8 runs over 8 samples · mean 4.04 Gbp per run (median 4.02 Gbp, total 32.29 Gbp; 8 runs with a base count) · layout PAIRED 8 · platform ILLUMINA 8 · models Illumina NovaSeq 6000 (8) · 88% of runs with a Sandpiper profile · first public 2024-07-11
Authors (0)
No author record.
Downloads
PRJNA1134113.csv.gz (1.1 KB) · parquet (69.9 KB) — all 8 samples, every field with route and confidence · PRJNA1134113_determinations.csv.gz (1.3 KB) — 56 evidence rows