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PRJNA1157208 SRP530547

L. plantarum 299v trial for GI aGvHD in pediatric alloHCT

Lactiplantibacillus plantarum for the Prevention of GI aGVHD. A Report from the Childrens Oncology Group

859 stool metagenome samples from children and adolescents undergoing allogeneic hematopoietic cell transplant, collected in a Phase 3 randomized, placebo-controlled trial of probiotic L. plantarum 299v for preventing GI acute graft-versus-host disease. machine-written summary

859 samples · 859 runs · US · Child · 2024 · 1.14 Gbp / sample

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Sources

✓Sequence archive 4,421○Abstract / description✓Full text 2,577○Supplementary tables–External resources–User contribution–Human verified

Checked this project's papers and archive records yourself? Mark it human-verified.

what each source gave

✓ Sequence archive	4,421 sample values (country, latitude, location region, longitude, subject id, timepoint)
○ Abstract / description	read; no usable statement
✓ Full text	2,577 sample values (collection date, health condition, health condition detail) · PMC13406991
○ Supplementary tables	files read; no table could be matched to the samples
– External resources	not covered by the external resources we ingest
– User contribution	nothing uploaded yet
– Human verified	not yet checked by a person · I checked this project — verify it

Interventions

intervention — arms not resolved

Probiotic (live microorganisms; single strain or mixture) design randomized_controlled_trial · population transplant_or_immunocompromised · L. plantarum 299v vs placebo for GI aGvHD prevention

“randomized, placebo-controlled clinical trial” — abstract:40306553 (study level; per-sample arms are in the intervention column below)

Samples at a glance

1 sampling locations · 1 country

Age

unknown859

Condition

transplant_or_immunocompromised859

Country

US859

Body site

primary859

Sequencing depth per sample · this project all projects

Sampling design and relatedness

longitudinal — not resolved

154 of 156 people sampled more than once (856 samples; median 6, up to 9 per person).

How longitudinal and relatedness information is derived

Community composition

Enterococcus_B 7.6%Blautia_A 7.5%Streptococcus 6.8%Bifidobacterium 5.8%Mediterraneibacter 4.7%Bacteroides 3.8%Lacticaseibacillus 3.7%Enterococcus 2.7%Lactiplantibacillus 2.3%Blautia 2.1%Staphylococcus 1.8%Enterocloster 1.7%not assigned to a genus 5.4%other genera 43.9%

Genera, mean relative abundance over 419 profiled gut samples of this project (Sandpiper SingleM profiles, GTDB R232; low-depth and non-metagenome profiles left out).

Field coverage

field	samples	coverage	R1	R3
age_category	0	0%
country	859	100%	859
health_condition	859	100%		859
subject_id	858	100%	858
collection_date	859	100%		859
detailed_location	859	100%
health_condition_detail	859	100%		859
latitude	859	100%	859
location_region	859	100%	859
longitude	859	100%	859
timepoint_label	127	15%	127

Papers (1)

A Randomized Clinical Trial Evaluating Lactiplantibacillus Plantarum for the Prevention of GI aGvHD: A Report From the Children's Oncology Group (ACCL1633). 2025 PMID 40306553 PMC13406991 own_data

Details

Archive description

This dataset includes shotgun metagenomic sequences from a Phase 3 clinical trial evaluating L. plantarum 299v for the prevention of GI aGvHD in children and adolescents undergoing alloHCT. Stool samples were collected at multiple timepoints, with participants randomized to receive either L. plantarum 299v or placebo. The sequences reflect the microbial diversity and changes observed during the trial, with the goal of understanding the probiotic impact on gastrointestinal integrity.

How this project entered the registry

Classified as human gut / stool shotgun DNA metagenome of child and adolescent by opus_adjudicated with confidence 0.65. Assay shotgun_dna.

host run.host_tax_id · “9606”
host study.description · “children and adolescents undergoing alloHCT”
body site study.description · “Stool samples were collected at multiple timepoints”
life stage study.description · “children and adolescents undergoing alloHCT”

Evidence examples (one per field × route)

sample	field	value	route	conf.	source	quote
SAMN43507745	collection_date	2018-09/2022-03	R3	0.70	paper.fulltext.methods	Recruitment took place from September 2018 to March 2022
SAMN43507745	country	US	R1	0.90	sample.attr.geo_loc_name	USA:NY
SAMN43507745	health_condition	transplant_or_immunocompromised	R3	0.70	paper.fulltext.methods	treatment with alternative donor alloHCT
SAMN43507745	health_condition_detail	patients undergoing alternative donor allogeneic hematopoietic cell transplant	R3	0.70	paper.fulltext.methods	treatment with alternative donor alloHCT
SAMN43507745	latitude	40.8075	R1	0.90	biosample.attribute:lat_lon	40.8075 N 73.9626 W
SAMN43507745	location_region	New York	R1	0.75	biosample.attribute:geo_loc_name	USA:NY
SAMN43507745	longitude	-73.9626	R1	0.90	biosample.attribute:lat_lon	40.8075 N 73.9626 W
SAMN43507745	subject_id	879183	R1	0.70	sample.name_rule:ena.sample_alias	879183D120
SAMN43507752	timepoint_label	SOC	R1	0.70	sample.name_rule:ena.sample_alias	879800SOC

Sequencing

859 runs over 859 samples · mean 1.38 Gbp per run (median 1.14 Gbp, total 1,186.83 Gbp; 859 runs with a base count) · layout PAIRED 518, SINGLE 341 · platform ILLUMINA 859 · models Illumina NovaSeq 6000 (518), NextSeq 550 (341) · 49% of runs with a Sandpiper profile · first public 2024-09-08

Authors (16)

Ladas EJ first · Collier W · Park H · Auletta JJ · Dvorak CC · August A · Esbenshade AJ · Bhatia M · Fisher BT · Levine JE · Pollock BH · Uhlemann AC · Verneris MR · Walters M · Yu L · Nieder M last

Downloads

PRJNA1157208.csv.gz (20.2 KB) · parquet (100.4 KB) — all 859 samples, every field with route and confidence · PRJNA1157208_determinations.csv.gz (40.9 KB) — 6,998 evidence rows

Samples (859 · every field this project has values for · hover a cell for its source)

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