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PRJNA1157371 SRP530819

Gut microbiome cross-sectional study in African women

AWI-Gen 2 Microbiome Project

1,820 samples from women aged 42-86 across Burkina Faso, Ghana, Kenya, and South Africa (AWI-Gen Wave 2 sub-study), examining the gut microbiome in a cross-sectional design. machine-written summary

1,820 samples · 1,820 runs · ZA, BF, KE · Adult · 2025 · 4.56 Gbp / sample

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Sources

✓Sequence archive 11,620○Abstract / description○Full text○Supplementary tables–External resources–User contribution–Human verified

Checked this project's papers and archive records yourself? Mark it human-verified.

what each source gave

✓ Sequence archive	11,620 sample values (collection date, country, latitude, location locality, location region, location site, longitude, subject id)
○ Abstract / description	read; no usable statement
○ Full text	read; no usable statement or table · PMC13483426
○ Supplementary tables	files read; no table could be matched to the samples
– External resources	not covered by the external resources we ingest
– User contribution	nothing uploaded yet
– Human verified	not yet checked by a person · I checked this project — verify it

Samples at a glance

6 sampling locations · 4 countries

Age

elderly1,820

Country

ZA962

BF384

KE239

GH235

Body site

primary1,820

Collected 2019–2022

Sequencing depth per sample · this project all projects

Sampling design and relatedness

longitudinal

239 of 917 people sampled more than once (1,142 samples; median 5, up to 5 per person) · follow-up median 2.3 y (max 3.2 y) · median interval between samples 5 mo (time from collection dates).

Each row is one person (36 shown of 239); dots are samples at days since that person's first sample.

How longitudinal and relatedness information is derived

Community composition

Prevotella 18.4%Faecalibacterium 3.4%Roseburia 2.9%Vescimonas 2.7%Cryptobacteroides 2.5%Blautia_A 2.3%Ruminococcoides 2.3%Succinivibrio 1.9%Hominicoprocola 1.8%Bacteroides 1.8%Bifidobacterium 1.4%Phocaeicola 1.3%not assigned to a genus 5.4%other genera 51.7%

Genera, mean relative abundance over 1,630 profiled gut samples of this project (Sandpiper SingleM profiles, GTDB R232; low-depth and non-metagenome profiles left out).

Field coverage

field	samples	coverage	R1
age_category	1,820	100%
country	1,820	100%	1,820
health_condition	0	0%
subject_id	1,820	100%	1,820
collection_date	1,820	100%	1,820
detailed_location	1,820	100%
latitude	1,820	100%	1,820
longitude	1,820	100%	1,820
location_locality	1,617	89%	1,617
location_region	700	38%	700
location_site	203	11%	203

Papers (1)

Participant engagement and feedback in microbiome projects: a case of AWI-Gen 2. 2026 PMID 42517623 PMC13483426 own_data

Details

Archive description

These data are part of the AWI-Gen 2 Microbiome Project, a large international study of the gut microbiome of approximately 1,800 women from Burkina Faso, Ghana, Kenya, and South Africa.

How this project entered the registry

Classified as human gut / stool shotgun DNA metagenome of adult by sonnet_x2 with confidence 0.75. Assay shotgun_dna.

host study.description · “gut microbiome of approximately 1,800 women”
body site study.description · “gut microbiome of approximately 1,800 women”
life stage study.description · “1,800 women from Burkina Faso, Ghana”

Evidence examples (one per field × route)

sample	field	value	route	conf.	source	quote
SAMN43510926	collection_date	2019-12-06	R1	0.90	biosample.attribute:collection_date	2019-12-06
SAMN43510926	country	ZA	R1	0.90	sample.attr.geo_loc_name	South Africa: Agincourt
SAMN43510926	latitude	-24.8200	R1	0.90	biosample.attribute:lat_lon	24.82 S 31.26 E
SAMN43510926	location_locality	Agincourt	R1	0.85	biosample.attribute:geo_loc_name	South Africa: Agincourt
SAMN43523415	location_region	Gauteng	R1	0.60	biosample.attribute:lat_lon	26.24 S 27.84 E
SAMN43527872	location_site	DIMAMO	R1	0.85	biosample.attribute:geo_loc_name	South Africa: DIMAMO
SAMN43510926	longitude	31.2600	R1	0.90	biosample.attribute:lat_lon	24.82 S 31.26 E
SAMN43510926	subject_id	AG001	R1	0.85	sample.attr.participant_id	AG001

Sequencing

1,820 runs over 1,820 samples · mean 4.60 Gbp per run (median 4.56 Gbp, total 8,375.81 Gbp; 1,820 runs with a base count) · layout PAIRED 1,820 · platform ILLUMINA 1,820 · models Illumina NovaSeq 6000 (1,820) · 90% of runs with a Sandpiper profile · first public 2025-01-04

Authors (20)

Nkera-Gutabara CK first · Olubayo LAI · Oduaran OO · Kisiangani I · Khoza S · Gama K · Maritze M · Mabunda C · Keya D · Adetunji KE · Tollman S · Micklesfield LK · Mohamed SF · Gómez-Olivé FX · Tluway F · Ramsay M · Bhatt AS · Hazelhurst S · Maghini DG · AWI-Gen Collaborative Centre and MADIVA Research Hub last

Downloads

PRJNA1157371.csv.gz (51.5 KB) · parquet (143.8 KB) — all 1,820 samples, every field with route and confidence · PRJNA1157371_determinations.csv.gz (82.1 KB) — 11,620 evidence rows

Samples (1,820 · every field this project has values for · hover a cell for its source)

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