Ex-vivo food additive effects on gut microbiome, Belgium
21 fecal samples from healthy donors in Belgium, used in short-term ex-vivo incubations to study effects of selected food additives or components on gut microbiome structure and function. machine-written summary
120 samples · 120 runs · BE · Infant / toddler · 2025 · 2.52 Gbp / sample
Checked this project's papers and archive records yourself? Mark it human-verified.
what each source gave
| ✓ Sequence archive | 522 sample values (collection date, country, health condition, health condition detail, subject id, timepoint) |
| ○ Abstract / description | read; no usable statement |
| ✓ Full text | 678 sample values (antibiotic exposure, health condition, health condition detail, location locality, location site, smoking status) · PMC12428287 |
| ○ Supplementary tables | files read; no table could be matched to the samples |
| – External resources | not covered by the external resources we ingest |
| – User contribution | nothing uploaded yet |
| – Human verified | not yet checked by a person · I checked this project — verify it |
intervention — arms not resolved
Other administered intervention (see intervention_detail) design observational_with_procedure · population healthy_control · Ex vivo fecal fermentation dosed with sodium benzoate food additive
“A dose of 3.5 g/L SB” — abstract:40941064 (study level; per-sample arms are in the intervention column below)
longitudinal
24 of 24 people sampled more than once (120 samples; median 5, up to 5 per person) · follow-up median 1 d (max 1 d) · median interval between samples 1 d (time from timepoint labels).
Each row is one person (24 shown); dots are samples at days since that person's first sample.
Genera, mean relative abundance over 21 profiled gut samples of this project (Sandpiper SingleM profiles, GTDB R232; low-depth and non-metagenome profiles left out).
| field | samples | coverage | R1 | R2 | R3 | R4 |
|---|---|---|---|---|---|---|
| age_category | 99 | 82% | ||||
| country | 120 | 100% | 120 | |||
| health_condition | 120 | 100% | 21 | 99 | ||
| subject_id | 120 | 100% | 120 | |||
| antibiotic_exposure | 120 | 100% | 120 | |||
| collection_date | 120 | 100% | 120 | |||
| detailed_location | 120 | 100% | ||||
| health_condition_detail | 120 | 100% | 21 | 99 | ||
| location_locality | 120 | 100% | 120 | |||
| location_site | 120 | 100% | 120 | |||
| smoking_status | 120 | 100% | 120 | |||
| timepoint_label | 120 | 100% | 120 |
- The Effect of Sodium Benzoate on the Gut Microbiome Across Age Groups. PMID 40941064 PMC12428287 curated
- The Effect of Sodium Benzoate on the Gut Microbiome Across Age Groups. 2025 PMID 40941064 PMC12428287 own_data
Archive description
This study applies short-term ex-vivo incubation technology to fecal samples from 24 healthy donors in four age groups (infants, toddlers, adults, older adults) to explore the effects of selected food additives or components on the structure and function of the human gut microbiome.
How this project entered the registry
Classified as human gut / stool shotgun DNA metagenome of infant / toddler and adult and elderly by deterministic_prior with confidence 0.90. Assay shotgun_dna.
- host external_curation.infant_catalog · “infant triage verdict include”
- body site study.title · “on the human gut microbiome”
- body site study.description · “incubation technology to fecal samples from 24”
- life stage external_curation.infant_catalog · “infant triage verdict include (0-3 y scope)”
- life stage study.description · “age groups ( infants , toddlers, adults,”
- life stage study.description · “groups (infants, toddlers, adults , older adults)”
- life stage study.description · “(infants, toddlers, adults, older adults ) to explore”
Evidence examples (one per field × route)
| sample | field | value | route | conf. | source | quote |
|---|---|---|---|---|---|---|
| SAMN43708632 | antibiotic_exposure | no | R3 | 0.70 | paper.body.methods | having consumed no anti-/pre-/probiotics for at least three months before their donations |
| SAMN43708632 | collection_date | 2023-04 | R1 | 0.85 | biosample.attribute:collection_date | collection_date: 2023-04 |
| SAMN43708632 | country | BE | R1 | 0.90 | sample.attr.geo_loc_name | Belgium |
| SAMN43708633 | country | BE | R1 | 0.90 | biosample_attr | Belgium |
| SAMN43708633 | health_condition | healthy_control | R1 | 0.85 | biosample.attribute:host_age_group | host_age_group: Toddler |
| SAMN43708632 | health_condition | healthy_control | R3 | 0.70 | paper.body.methods | non-smokers, no GIT disorders or cancer, taking no medicine for allergies or psychological disorders |
| SAMN43708633 | health_condition_detail | Toddler | R1 | 0.85 | biosample.attribute:host_age_group | host_age_group: Toddler |
| SAMN43708632 | health_condition_detail | non-smokers, no GIT disorders or cancer, taking no medicine for allergies or psychological disorders | R3 | 0.70 | paper.body.methods | non-smokers, no GIT disorders or cancer, taking no medicine for allergies or psychological disorders |
| SAMN43708632 | location_locality | Ghent | R3 | 0.70 | paper.fulltext.methods | University Hospital Ghent, Belgium |
| SAMN43708632 | location_site | University Hospital Ghent | R3 | 0.70 | paper.fulltext.methods | Ethics Committee of the University Hospital Ghent |
| SAMN43708632 | smoking_status | never | R3 | 0.70 | paper.body.methods | non-smokers, no GIT disorders or cancer |
| SAMN43708632 | subject_id | Donor_10 | R1 | 0.85 | biosample.attribute:subject_id | subject_id: Donor_10 |
| SAMN43708633 | subject_id | Donor_10 | R1 | 0.85 | biosample_attr | Donor_10 |
| SAMN43708632 | timepoint_label | 24h | R1 | 0.85 | biosample.attribute:incubation_timepoint | incubation_timepoint: 24h |
Sequencing
120 runs over 120 samples · mean 2.44 Gbp per run (median 2.52 Gbp, total 293.31 Gbp; 120 runs with a base count) · layout PAIRED 120 · platform ILLUMINA 120 · models Illumina NovaSeq 6000 (120) · 100% of runs with a Sandpiper profile · first public 2025-04-27
Authors (11)
Lemons JMS first · Narrowe AB · Firrman J · Mahalak KK · Liu L · Higgins S · Moustafa AM · Baudot A · Deyaert S · Van den Abbeele P last · Chetty VJ
Downloads
PRJNA1160256.csv.gz (4.2 KB) · parquet (75.7 KB) — all 120 samples, every field with route and confidence · PRJNA1160256_determinations.csv.gz (9.0 KB) — 1,200 evidence rows