2'-FL prebiotic RCT in older adults, Palo Alto, USA
317 samples from a 6-week randomized controlled trial in Palo Alto, California, USA, testing 2'-fucosyllactose (a human milk oligosaccharide prebiotic) versus placebo in 89 healthy older adults (mean age 67.3). machine-written summary
317 samples · 317 runs · US · Elderly · 2024 · 3.82 Gbp / sample
Checked this project's papers and archive records yourself? Mark it human-verified.
what each source gave
| ✓ Sequence archive | 3,487 sample values (age, collection date, country, intervention, latitude, location locality, location region, longitude) |
| ✓ Abstract / description | 634 sample values (health condition, health condition detail) |
| ○ Full text | read; no usable statement or table · PMC12432366 |
| ○ Supplementary tables | files read; no table could be matched to the samples |
| – External resources | not covered by the external resources we ingest |
| – User contribution | nothing uploaded yet |
| – Human verified | not yet checked by a person · I checked this project — verify it |
intervention · arms resolved
Prebiotic / dietary fibre supplement design randomized_controlled_trial · population healthy_control · 2'-fucosyllactose (HMO) two doses vs placebo, 6 weeks
“consumed at either of two doses versus placebo by 89 healthy older” — bioproject.description (study level; per-sample arms are in the intervention column below)
longitudinal
84 of 84 people sampled more than once (317 samples; median 4, up to 4 per person) · follow-up median 56 d (max 14 mo) · median interval between samples 21 d (time from collection dates). Schedule stated by the source: “Stool and blood collected at weeks -2, 0, 3, 6, and 10” (time origin: intervention_start).
Each row is one person (36 shown of 84); dots are samples at days since that person's first sample.
Genera, mean relative abundance over 289 profiled gut samples of this project (Sandpiper SingleM profiles, GTDB R232; low-depth and non-metagenome profiles left out).
| field | samples | coverage | R1 | R2 | R3 | R4 |
|---|---|---|---|---|---|---|
| age_category | 317 | 100% | ||||
| country | 317 | 100% | 317 | |||
| health_condition | 317 | 100% | 317 | |||
| subject_id | 317 | 100% | 317 | |||
| age_at_collection_days | 317 | 100% | 317 | |||
| collection_date | 317 | 100% | 317 | |||
| detailed_location | 317 | 100% | ||||
| health_condition_detail | 317 | 100% | 317 | |||
| intervention | 317 | 100% | 317 | |||
| latitude | 317 | 100% | 317 | |||
| location_locality | 317 | 100% | 317 | |||
| location_region | 317 | 100% | 317 | |||
| longitude | 317 | 100% | 317 | |||
| sex | 317 | 100% | 317 | |||
| timepoint_label | 317 | 100% | 317 |
- A human milk oligosaccharide alters the microbiome, circulating hormones, and metabolites in a randomized controlled trial of older adults. 2025 PMID 40738103 PMC12432366 own_data
Archive description
Aging-related remodeling in immune function is associated with diseases like cancer, atherosclerosis, and neurodegenerative conditions. This study aimed to improve the aging gut microbiota and immune system by introducing 2-fucosyllactose (2'-FL), a common oligosaccharide found in human breast milk with established health benefits in infants and animal models. 2'-FL was consumed at either of two doses versus placebo by 89 healthy older individuals (average age = 67.3 years) in a 6-week randomized controlled trial. Although the primary endpoint (significant change in the cytokine response score) was not met, consumers of the prebiotic experienced increased levels of Bifidobacterium in the gut microbiota, along with elevated serum levels of insulin, high-density lipoprotein (HDL) cholesterol, and fibroblast growth factor 21 (FGF21) hormone. Additional metabolomic and proteomic changes were observed specifically in 2'-FL "responders" who experienced a bloom in Bifidobacterium. Multi-omics analysis indicated a systemic response to 2'-FL, which could be detected in blood and urine, showcasing the potential of this prebiotic to provide diverse benefits for healthy aging.
How this project entered the registry
Classified as human gut / stool shotgun DNA metagenome of elderly and adult by sonnet_x2 with confidence 0.65. Assay shotgun_dna.
- host run.host_tax_id · “9606”
- body site run.scientific_name · “gut metagenome”
- life stage study.description · “89 healthy older individuals (average age = 67.3 years)”
- life stage sample.attr.age · “61 (n=95/317)”
Evidence examples (one per field × route)
| sample | field | value | route | conf. | source | quote |
|---|---|---|---|---|---|---|
| SAMN44455394 | age_at_collection_days | 25348.4 | R1 | 0.70 | sample.attr.age | 69.4 |
| SAMN44455394 | collection_date | 2019-04-16 | R1 | 0.90 | biosample.attribute:collection_date | 2019-04-16 |
| SAMN44455394 | country | US | R1 | 0.90 | sample.attr.geo_loc_name | USA: California, Palo Alto |
| SAMN44455394 | health_condition | healthy_control | R4 | 0.50 | study.description | 89 healthy older individuals |
| SAMN44455394 | health_condition_detail | Healthy older individuals | R4 | 0.50 | study.description | 89 healthy older individuals |
| SAMN44455394 | intervention | prebiotic_or_fibre | R1 | 0.85 | biosample.attribute:final_grp | High Dose 2FL |
| SAMN44455394 | latitude | 37.4360 | R1 | 0.90 | biosample.attribute:lat_lon | 37.436 N 122.176 W |
| SAMN44455394 | location_locality | Palo Alto | R1 | 0.85 | biosample.attribute:geo_loc_name | USA: California, Palo Alto |
| SAMN44455394 | location_region | California | R1 | 0.85 | biosample.attribute:geo_loc_name | USA: California, Palo Alto |
| SAMN44455394 | longitude | -122.1760 | R1 | 0.90 | biosample.attribute:lat_lon | 37.436 N 122.176 W |
| SAMN44455394 | sex | male | R1 | 0.90 | sample.attr.gender | male |
| SAMN44455394 | subject_id | 4000 | R1 | 0.85 | sample.attr.participant_id | 4000 |
| SAMN44455394 | timepoint_label | 1 | R1 | 0.85 | sample.attr.timepoint | 1 |
Sequencing
317 runs over 317 samples · mean 4.04 Gbp per run (median 3.82 Gbp, total 1,281.94 Gbp; 317 runs with a base count) · layout PAIRED 317 · platform ILLUMINA 317 · models Illumina NovaSeq 6000 (317) · 91% of runs with a Sandpiper profile · first public 2024-10-27
Authors (15)
Carter MM first · Demis D · Perelman D · St Onge M · Petlura C · Cunanan K · Mathi K · Maecker HT · Chow JM · Robinson JL · Sabag-Daigle A · Sonnenburg ED · Buck RH · Gardner CD · Sonnenburg JL last
Downloads
PRJNA1177009.csv.gz (11.0 KB) · parquet (84.9 KB) — all 317 samples, every field with route and confidence · PRJNA1177009_determinations.csv.gz (25.0 KB) — 4,121 evidence rows