Gut microbiome in non-celiac gluten sensitivity, probiotic trial
60 samples from adults with non-celiac gluten wheat sensitivity, randomized to probiotic (L. plantarum, L. paracasei, L. salivarius) or placebo for 6 weeks, sampled during gluten-free diet and after gluten reintroduction. machine-written summary
60 samples · 60 runs · Adult · 2025 · 9.73 Gbp / sample
Checked this project's papers and archive records yourself? Mark it human-verified.
what each source gave
| ✓ Sequence archive | 240 sample values (collection date, intervention, subject id, timepoint) |
| – Abstract / description | no abstract or project description |
| – Full text | no open-access full text linked |
| – Supplementary tables | no supplementary tables available (or none open access) |
| – External resources | not covered by the external resources we ingest |
| – User contribution | nothing uploaded yet · share metadata |
| – Human verified | not yet checked by a person · I checked this project — verify it |
intervention · arms resolved
4 people have samples in more than one arm.
Controlled / prescribed diet or feeding regimen Probiotic (live microorganisms; single strain or mixture) design randomized_controlled_trial · population other_disease · Probiotic (L. plantarum, paracasei, salivarius) vs placebo, plus gluten-free then reintroduction diet
“daily intake of commericial probiotics” — bioproject.description (study level; per-sample arms are in the intervention column below)
longitudinal
30 of 30 people sampled more than once (60 samples; median 2, up to 2 per person) · follow-up median 14 d (max 14 d) · median interval between samples 14 d (time from visit codes mapped to the stated schedule). Schedule stated by the source: “Sampled at T1 (end of 4-week gluten-free diet) and T2 (after 2-week gluten reintroduction), per patient, under probiotic or placebo arm.” (time origin: other: start of gluten reintroduction follows 4-week gluten-free run-in; T1 set as origin); stated follow-up 14 d.
Each row is one person (18 shown of 30); dots are samples at days since that person's first sample.
Genera, mean relative abundance over 60 profiled gut samples of this project (Sandpiper SingleM profiles, GTDB R232; low-depth and non-metagenome profiles left out).
| field | samples | coverage | R1 | R2 | R3 | R4 |
|---|---|---|---|---|---|---|
| age_category | 60 | 100% | ||||
| country | 0 | 0% | ||||
| health_condition | 0 | 0% | ||||
| subject_id | 60 | 100% | 60 | |||
| collection_date | 60 | 100% | 60 | |||
| intervention | 60 | 100% | 60 | |||
| timepoint_label | 60 | 100% | 60 |
Archive description
participants with Non-celiac gluten wheat sensitivity were randomized into two arms: Experimental arm: 6 weeks with daily intake of commericial probiotics (L. plantarum, L. paracasei and L. salivarius) or Control arm: 6 weeks with daily intake of placebo. All participants were undergoing a gluten-free diet for 4 weeks (sampling point T1), after which gluten was reintroduced into their diets for 2 weeks (sampling point T2)
How this project entered the registry
Classified as human gut / stool / oral cavity shotgun DNA metagenome of adult by deterministic_prior with confidence 0.90. Assay shotgun_dna.
- host external_curation.infant_catalog · “infant triage reason_code age_adult_only”
- body site study.title · “Gut microbiome of individuals”
- body site study.description · “paracasei and L. salivarius ) or Control”
- life stage external_curation.infant_catalog · “infant triage reason_code age_adult_only”
Evidence examples (one per field × route)
| sample | field | value | route | conf. | source | quote |
|---|---|---|---|---|---|---|
| SAMN44706601 | collection_date | 2021 | R1 | 0.90 | biosample.attribute:collection_date | 2021 |
| SAMN44706601 | intervention | placebo | R1 | 0.70 | sample.name_rule:title | Shotgun metagenomic analysis of gut microbiota of NCGS patiet 1 sampling time T1 treatment Placebo |
| SAMN44706601 | subject_id | 1 | R1 | 0.70 | sample.name_rule:title | Shotgun metagenomic analysis of gut microbiota of NCGS patiet 1 sampling time T1 treatment Placebo |
| SAMN44706601 | timepoint_label | T1 | R1 | 0.70 | sample.name_rule:title | Shotgun metagenomic analysis of gut microbiota of NCGS patiet 1 sampling time T1 treatment Placebo |
Sequencing
60 runs over 60 samples · mean 10.33 Gbp per run (median 9.73 Gbp, total 619.62 Gbp; 60 runs with a base count) · layout PAIRED 60 · platform ILLUMINA 60 · models NextSeq 550 (60) · 100% of runs with a Sandpiper profile · first public 2025-12-04
Authors (0)
No author record.
Help complete this project
2 of the 4 core fields are below 50% coverage: country, health_condition. Share metadata
Downloads
PRJNA1183595.csv.gz (2.3 KB) · parquet (71.0 KB) — all 60 samples, every field with route and confidence · PRJNA1183595_determinations.csv.gz (2.5 KB) — 240 evidence rows