Legume diet RCT in Singapore prediabetes adults
662 samples from Chinese prediabetes adults in Singapore, from a 16-week randomized controlled trial comparing a calorie-restricted, legume-enriched diet versus a calorie-restricted control diet. machine-written summary
662 samples · 662 runs · SG · Adult · 2024 · 7.93 Gbp / sample
Checked this project's papers and archive records yourself? Mark it human-verified.
what each source gave
| ✓ Sequence archive | 4,634 sample values (collection date, country, latitude, location locality, longitude, subject id, timepoint) |
| ○ Abstract / description | read; no usable statement |
| ○ Full text | read; no usable statement or table · PMC11754483 |
| ○ Supplementary tables | files read; no table could be matched to the samples |
| – External resources | not covered by the external resources we ingest |
| – User contribution | nothing uploaded yet |
| – Human verified | not yet checked by a person · I checked this project — verify it |
intervention — arms not resolved
Controlled / prescribed diet or feeding regimen design randomized_controlled_trial · population type2_diabetes · Calorie-restricted, legume-enriched diet vs calorie-restricted control diet
“Legume-enriched diet improves metabolic health in prediabetes” — study.title (study level; per-sample arms are in the intervention column below)
longitudinal
111 of 111 people sampled more than once (662 samples; median 6, up to 6 per person) · follow-up median 4 mo (max 4 mo) · median interval between samples 28 d (time from timepoint labels). Schedule stated by the source: “Fecal samples collected at baseline, week 2, week 4, week 8, week 12, and week 16” (time origin: baseline); stated follow-up 4 mo.
Each row is one person (36 shown of 111); dots are samples at days since that person's first sample.
Genera, mean relative abundance over 609 profiled gut samples of this project (Sandpiper SingleM profiles, GTDB R232; low-depth and non-metagenome profiles left out).
| field | samples | coverage | R1 | R2 | R3 | R4 |
|---|---|---|---|---|---|---|
| age_category | 662 | 100% | ||||
| country | 662 | 100% | 662 | |||
| health_condition | 0 | 0% | ||||
| subject_id | 662 | 100% | 662 | |||
| collection_date | 662 | 100% | 662 | |||
| detailed_location | 662 | 100% | ||||
| latitude | 662 | 100% | 662 | |||
| location_locality | 662 | 100% | 662 | |||
| longitude | 662 | 100% | 662 | |||
| timepoint_label | 662 | 100% | 662 |
- A legume-enriched diet improves metabolic health in prediabetes mediated through gut microbiome: a randomized controlled trial. 2025 PMID 39843443 PMC11754483 own_data
- Cross-body site microbial interactions influence the human plasma metabolome. 2026 PMID 41952172 PMC13181886 unsure
Archive description
Healthy dietary patterns rich in legumes can improve metabolic health, although their additional benefits in conjunction with calorie restriction has not been well-established. We investigated effects of a calorie-restricted, legume-enriched, multicomponent intervention diet compared with a calorie-restricted control diet in 127 Chinese prediabetes participants, living in Singapore. The study was a 16-week, single-blind, parallel-design, randomized controlled trial (n=63 intervention group (IG), n=64 control group (CG)). Primary outcomes were markers of glycemia and all measurements were taken at 2 or 4-weekly intervals. At the end of 16 weeks, both groups had significantly lower BMI compared with baseline, with minimal difference between groups. The IG had significantly greater reductions in LDL cholesterol, total cholesterol and HbA1c compared with CG, alongside increases in fibre degrading species in IG, mediated through metabolites such as bile acids and amino acids. A legume-enriched, multicomponent intervention diet can improve metabolic health in a prediabetes population, in addition to benefits obtained from calorie restriction alone, partially mediated through changes in gut microbial composition and function.</p><p>Trial registration: Clinical Trials NCT04745702.
How this project entered the registry
Classified as human gut / stool shotgun DNA metagenome of adult by sonnet_x2 with confidence 0.72. Assay shotgun_dna.
- host study.description · “127 Chinese prediabetes participants”
- body site run.scientific_name · “human gut metagenome”
- life stage study.description · “calorie-restricted, legume-enriched, multicomponent intervention diet”
Evidence examples (one per field × route)
| sample | field | value | route | conf. | source | quote |
|---|---|---|---|---|---|---|
| SAMN45930898 | collection_date | 2021 | R1 | 0.90 | biosample.attribute:collection_date | 2021 |
| SAMN45930898 | country | SG | R1 | 0.90 | sample.attr.geo_loc_name | Singapore |
| SAMN45930898 | latitude | 1.3521 | R1 | 0.90 | biosample.attribute:lat_lon | 1.3521 N 103.8198 E |
| SAMN45930898 | location_locality | Singapore | R1 | 0.85 | biosample.attribute:geo_loc_name | Singapore |
| SAMN45930898 | longitude | 103.8198 | R1 | 0.90 | biosample.attribute:lat_lon | 1.3521 N 103.8198 E |
| SAMN45930898 | subject_id | FBI128 | R1 | 0.70 | sample.name_rule:source_material_id | FBI128, Group1, W16 |
| SAMN45930898 | timepoint_label | week 16 | R1 | 0.70 | sample.name_rule:source_material_id | FBI128, Group1, W16 |
Sequencing
662 runs over 662 samples · mean 8.17 Gbp per run (median 7.93 Gbp, total 5,409.48 Gbp; 662 runs with a base count) · layout PAIRED 662 · platform ILLUMINA 662 · models Illumina NovaSeq X (662) · 92% of runs with a Sandpiper profile · first public 2024-12-23 – 2024-12-29
Authors (27)
Wu X first · Tjahyo AS · Volchanskaya VSB · Wong LH · Lai X · Yong YN · Osman F · Tay SL · Govindharajulu P · Ponnalagu S · Tso R · Teo HS · Khoo K · Fan H · Goh CC · Yap CPL · Leow MK · Henry CJ · Haldar S · Lim KJ last · Zhang J first · Jiang C · Zhou X · Gao P · Wong S · Snyder M · Shen X last
Downloads
PRJNA1201249.csv.gz (16.4 KB) · parquet (91.6 KB) — all 662 samples, every field with route and confidence · PRJNA1201249_determinations.csv.gz (33.1 KB) — 4,634 evidence rows