Gut microbiome in ICU staff vs non-medical adults, Wuhan
Cross-sectional study of 78 fecal samples from adults in Wuhan, China, comparing gut microbiome structure and function between ICU staff and non-medical control subjects. machine-written summary
78 samples · 78 runs · CN · Adult · 2026 · 12.66 Gbp / sample
Checked this project's papers and archive records yourself? Mark it human-verified.
what each source gave
| ✓ Sequence archive | 468 sample values (collection date, country, latitude, location locality, location region, longitude) |
| ○ Abstract / description | read; no usable statement |
| ✓ Full text | 234 sample values (antibiotic exposure, health condition, health condition detail, subject id) · PMC11959758 |
| ✓ Supplementary tables | 234 sample values (health condition, health condition detail, sex, shared setting id, shared setting type) |
| – External resources | not covered by the external resources we ingest |
| – User contribution | nothing uploaded yet |
| – Human verified | not yet checked by a person · I checked this project — verify it |
workplace
39 ICU staff from one ICU (shared workplace) vs 39 non-medical controls1 shared settings.
member · circles are people (pale = no sequenced sample), arrows run donor → recipient and parent → child, curved lines join twins, partners and siblings, squares are shared settings or families without recorded roles. Shown: 1 shared setting. 78 people in the project overall.
Genera, mean relative abundance over 77 profiled gut samples of this project (Sandpiper SingleM profiles, GTDB R232; low-depth and non-metagenome profiles left out).
| field | samples | coverage | R1 | R2 | R3 | R4 |
|---|---|---|---|---|---|---|
| age_category | 78 | 100% | ||||
| country | 78 | 100% | 78 | |||
| health_condition | 78 | 100% | 39 | 39 | ||
| subject_id | 78 | 100% | 78 | |||
| antibiotic_exposure | 78 | 100% | 78 | |||
| collection_date | 78 | 100% | 78 | |||
| detailed_location | 78 | 100% | ||||
| health_condition_detail | 78 | 100% | 39 | 39 | ||
| latitude | 78 | 100% | 78 | |||
| location_locality | 78 | 100% | 78 | |||
| location_region | 78 | 100% | 78 | |||
| longitude | 78 | 100% | 78 | |||
| sex | 78 | 100% | 78 | |||
| shared_setting_id | 39 | 50% | 39 | |||
| shared_setting_type | 39 | 50% | 39 |
- Structural and functional alteration of the gut microbiomes in ICU staff: a cross-sectional analysis. 2025 PMID 40165255 PMC11959758 own_data
Archive description
This study aimed to further compare the taxonomic and functional characteristics of gut microbiomes between ICU staff and non-medical subjects using metagenomic sequencing. A cross-sectional cohort study was conducted prospectively in an ICU in Wuhan, wherein fecal samples from 39 individuals per group were analyzed through metagenomic sequencing. We found that ICU staff demonstrated a significant reduction in gut microbiome diversity when compared to non-medical controls, characterized by a prominent reduction in Actinobacteria and a significant increase in Bacteroides and Bacteroidaceae. KEGG analysis revealed an elevated risk of endocrine and metabolic diseases, accompanied by an augmentation in glycan biosynthesis and metabolism.
How this project entered the registry
Classified as human gut / stool shotgun DNA metagenome of adult by opus_adjudicated with confidence 0.75. Assay shotgun_dna.
- host run.host_tax_id · “9606”
- host run.scientific_name · “human metagenome”
- body site study.description · “fecal samples from 39 individuals per group”
- life stage study.title · “gut microbiomes in ICU staff”
Evidence examples (one per field × route)
| sample | field | value | route | conf. | source | quote |
|---|---|---|---|---|---|---|
| SAMN46186991 | antibiotic_exposure | no | R3 | 0.70 | paper.body.methods_results | use of antibiotics, proton pump inhibitors, prebiotics, and probiotics (including yogurt) in the last 4 weeks |
| SAMN46186991 | collection_date | 2023-11-01 | R1 | 0.90 | biosample.attribute:collection_date | 2023-11-01 |
| SAMN46186991 | country | CN | R1 | 0.90 | sample.attr.geo_loc_name | China: Wuhan |
| SAMN46186991 | health_condition | healthy_control | R2 | 0.80 | paper.supp.13054_2025_5379_MOESM10_ESM.xls[Gender code!Group] | Non-medical controls |
| SAMN46186993 | health_condition | healthy_control | R3 | 0.70 | paper.body.methods_results | This study included 78 healthy Chinese participants, comprising 39 ICU staff and 39 non-medical controls. |
| SAMN46186991 | health_condition_detail | Non-medical controls | R2 | 0.80 | paper.supp.13054_2025_5379_MOESM10_ESM.xls[Gender code!Group] | Non-medical controls |
| SAMN46186993 | health_condition_detail | healthy participants | R3 | 0.70 | paper.body.methods_results | This study included 78 healthy Chinese participants, comprising 39 ICU staff and 39 non-medical controls. |
| SAMN46186991 | latitude | 30.5800 | R1 | 0.90 | biosample.attribute:lat_lon | 30.58 N 114.27 E |
| SAMN46186991 | location_locality | Wuhan | R1 | 0.85 | biosample.attribute:geo_loc_name | China: Wuhan |
| SAMN46186991 | location_region | Hubei | R1 | 0.60 | biosample.attribute:lat_lon | 30.58 N 114.27 E |
| SAMN46186991 | longitude | 114.2700 | R1 | 0.90 | biosample.attribute:lat_lon | 30.58 N 114.27 E |
| SAMN46186991 | sex | female | R2 | 0.80 | paper.supp.13054_2025_5379_MOESM10_ESM.xls[Gender code!Gender] | Female |
| SAMN46186993 | shared_setting_id | Union Hospital adult mixed medical-surgical ICU | R2 | 0.85 | paper.supp.13054_2025_5379_MOESM1_ESM.xls[Gender code!ICU staff] | ICU staff: S20240328228 |
| SAMN46186993 | shared_setting_type | workplace | R2 | 0.85 | paper.supp.13054_2025_5379_MOESM1_ESM.xls[Gender code!ICU staff] | ICU staff: S20240328228 |
| SAMN46186991 | subject_id | SAMN46186991 | R3 | 0.70 | design.one_sample_per_subject:fulltext | This study included 78 healthy Chinese participants, comprising 39 ICU staff and 39 non-medical controls. |
Sequencing
78 runs over 78 samples · mean 12.68 Gbp per run (median 12.66 Gbp, total 989.17 Gbp; 78 runs with a base count) · layout PAIRED 78 · platform ILLUMINA 78 · models Illumina NovaSeq 6000 (78) · 99% of runs with a Sandpiper profile · first public 2026-01-07
Authors (0)
No author record.
Downloads
PRJNA1208576.csv.gz (3.3 KB) · parquet (75.5 KB) — all 78 samples, every field with route and confidence · PRJNA1208576_determinations.csv.gz (7.6 KB) — 936 evidence rows