Longitudinal Gut Microbiome Stability in Older Adults, Seattle
120 fecal samples from healthy older adults in Seattle, USA, collected every six months over two years to assess temporal stability of the gut microbiome; a longitudinal design. machine-written summary
120 samples · 120 runs · US · Adult · 2025 · 2.72 Gbp / sample
Checked this project's papers and archive records yourself? Mark it human-verified.
what each source gave
| ✓ Sequence archive | 700 sample values (collection date, country, latitude, location locality, location region, longitude) |
| ✓ Abstract / description | 245 sample values (collection date, health condition, health condition detail) |
| ✓ Full text | 123 sample values (antibiotic exposure, country) · PMC12247528 |
| ○ Supplementary tables | files read; no table could be matched to the samples |
| – External resources | not covered by the external resources we ingest |
| – User contribution | nothing uploaded yet |
| – Human verified | not yet checked by a person · I checked this project — verify it |
longitudinal — not resolved
Schedule stated by the source: “every 6 months over a 2-year period (five samples)” (time origin: enrolment); stated follow-up 2.0 y.
Genera, mean relative abundance over 119 profiled gut samples of this project (Sandpiper SingleM profiles, GTDB R232; low-depth and non-metagenome profiles left out).
| field | samples | coverage | R1 | R2 | R3 | R4 |
|---|---|---|---|---|---|---|
| age_category | 120 | 100% | ||||
| country | 120 | 100% | 117 | 3 | ||
| health_condition | 120 | 100% | 120 | |||
| subject_id | 0 | 0% | ||||
| antibiotic_exposure | 120 | 100% | 120 | |||
| collection_date | 120 | 100% | 115 | 5 | ||
| health_condition_detail | 120 | 100% | 120 | |||
| detailed_location | 117 | 98% | ||||
| latitude | 117 | 98% | 117 | |||
| location_locality | 117 | 98% | 117 | |||
| location_region | 117 | 98% | 117 | |||
| longitude | 117 | 98% | 117 |
- Stability and Variability of the Human Fecal Microbiome over 2 Years in the Multiethnic Cohort Study: A Metagenomic Analysis. 2025 PMID 40402042 PMC12247528 own_data
Archive description
This study explores how stable the gut microbiome is over a two-year period in a group of healthy older adults. Researchers analyzed stool samples collected every six months using advanced sequencing techniques. The findings reveal that while most gut bacteria remained consistent over time, rarer microbial species and functions showed more variability. These insights highlight the importance of reliable measurement in microbiome studies related to cancer and suggest the need for sampling strategies to minimize bias in cancer risk estimation.
How this project entered the registry
Classified as human gut / stool shotgun DNA metagenome of adult and elderly by deterministic_prior with confidence 0.90. Assay shotgun_dna.
- host external_curation.infant_catalog · “infant triage reason_code age_unknown_no_evidence”
- body site study.title · “Stability of the Fecal Microbiome in Older”
- body site study.description · “how stable the gut microbiome is over”
- life stage study.title · “Microbiome in Older Adults”
- life stage study.description · “of healthy older adults . Researchers analyzed”
- life stage study.title · “Fecal Microbiome in Older Adults”
- life stage study.description · “group of healthy older adults . Researchers analyzed”
Evidence examples (one per field × route)
| sample | field | value | route | conf. | source | quote |
|---|---|---|---|---|---|---|
| SAMN09656864 | antibiotic_exposure | no | R3 | 0.70 | paper.fulltext.methods | confirmed to be free from antibiotic treatment at the time of enrollment |
| SAMN09656864 | collection_date | 2014-04-29 | R1 | 0.90 | biosample.attribute:collection_date | 2014-04-29 |
| SAMN09657064 | collection_date | 2013/2016 | R4 | 0.40 | paper.abstract | Adiposity Phenotype Study (2013-2016) |
| SAMN09656864 | country | US | R1 | 0.90 | sample.attr.geo_loc_name | USA:Seattle |
| SAMN46251828 | country | US | R3 | 0.70 | paper.fulltext.abstract | Multiethnic Cohort Adiposity Phenotype Study |
| SAMN09656864 | health_condition | healthy_control | R4 | 0.50 | study.description | group of healthy older adults |
| SAMN09656864 | health_condition_detail | Healthy older adults with no disease condition mentioned. | R4 | 0.50 | study.description | group of healthy older adults |
| SAMN09656864 | latitude | 47.6097 | R1 | 0.90 | biosample.attribute:lat_lon | 47.60972 N 122.33317 W |
| SAMN09656864 | location_locality | Seattle | R1 | 0.85 | biosample.attribute:geo_loc_name | USA:Seattle |
| SAMN09656864 | location_region | Washington | R1 | 0.60 | biosample.attribute:lat_lon | 47.60972 N 122.33317 W |
| SAMN09656864 | longitude | -122.3332 | R1 | 0.90 | biosample.attribute:lat_lon | 47.60972 N 122.33317 W |
Sequencing
120 runs over 120 samples · mean 2.85 Gbp per run (median 2.72 Gbp, total 342.01 Gbp; 120 runs with a base count) · layout PAIRED 120 · platform ILLUMINA 120 · models Illumina NovaSeq 6000 (120) · 99% of runs with a Sandpiper profile · first public 2025-05-04 – 2026-02-23
Authors (11)
Moosavi D first · Curtis KR · Randolph TW · Kahsai OJ · Ammar H · Lim U · Cheng I · Wilkens LR · Le Marchand L · Lampe JW · Hullar MAJ last
Downloads
PRJNA1210004.csv.gz (4.2 KB) · parquet (75.8 KB) — all 120 samples, every field with route and confidence · PRJNA1210004_determinations.csv.gz (9.3 KB) — 1,068 evidence rows