Diet intervention gut metagenome in healthy Italian adults
253 samples from healthy adult volunteers in Italy, studying effects of pulses-based or plant-based meat substitute diets versus habitual diet on the gut metagenome, in a three-arm intervention design. machine-written summary
253 samples · 253 runs · IT · Adult · 2025 · 10.58 Gbp / sample
Checked this project's papers and archive records yourself? Mark it human-verified.
what each source gave
| ✓ Sequence archive | 1,856 sample values (collection date, country, diet, diet detail, intervention, intervention detail, subject id, timepoint) |
| ✓ Abstract / description | 506 sample values (health condition, health condition detail) |
| – Full text | no open-access full text linked |
| – Supplementary tables | no supplementary tables available (or none open access) |
| – External resources | not covered by the external resources we ingest |
| – User contribution | nothing uploaded yet |
| – Human verified | not yet checked by a person · I checked this project — verify it |
intervention · arms resolved
Controlled / prescribed diet or feeding regimen design randomized_controlled_trial · population healthy_control · Pulses-enriched vs pulses+meat-surrogate vs habitual diet, randomized
“were randomized in three groups” — bioproject.description (study level; per-sample arms are in the intervention column below)
longitudinal
84 of 86 people sampled more than once (251 samples; median 3, up to 3 per person) · follow-up median 2 d (max 2 d) · median interval between samples 1 d (time from collection dates).
Each row is one person (36 shown of 84); dots are samples at days since that person's first sample.
Genera, mean relative abundance over 234 profiled gut samples of this project (Sandpiper SingleM profiles, GTDB R232; low-depth and non-metagenome profiles left out).
| field | samples | coverage | R1 | R2 | R3 | R4 |
|---|---|---|---|---|---|---|
| age_category | 253 | 100% | ||||
| country | 253 | 100% | 253 | |||
| health_condition | 253 | 100% | 253 | |||
| subject_id | 253 | 100% | 253 | |||
| collection_date | 253 | 100% | 253 | |||
| health_condition_detail | 253 | 100% | 253 | |||
| intervention | 253 | 100% | 253 | |||
| intervention_detail | 253 | 100% | 253 | |||
| timepoint_label | 253 | 100% | 253 | |||
| diet | 169 | 67% | 169 | |||
| diet_detail | 169 | 67% | 169 |
Archive description
Volunteers participating to the trial were randomized in three groups: PulD consumed a personalized, isocaloric and isoprotein pulses-enriched diet, replacing habitual servings of red/processed meat with pulses PPD consumed a personalized isocaloric and isoprotein diet, replacing habitual servings of red/processed meat with a mixture of pulses and plant-based meat surrogates HabD consumed their habitual diet.
How this project entered the registry
Classified as human gut / stool shotgun DNA metagenome of adult by deterministic_prior with confidence 0.90. Assay shotgun_dna.
- host external_curation.infant_catalog · “infant triage reason_code age_adult_only”
- body site study.title · “proteins on the gut metagenome”
- life stage external_curation.infant_catalog · “infant triage reason_code age_adult_only”
- life stage study.description · “Volunteers participating to the”
Evidence examples (one per field × route)
| sample | field | value | route | conf. | source | quote |
|---|---|---|---|---|---|---|
| SAMN46298609 | collection_date | 2024-04-01 | R1 | 0.90 | biosample.attribute:collection_date | 2024-04-01 |
| SAMN46298609 | country | IT | R1 | 0.90 | sample.attr.geo_loc_name | Italy |
| SAMN46298675 | diet | therapeutic_or_study_diet | R1 | 0.90 | biosample.attribute:special_diet_details | PulD: pulses-enriched diet (surrogating red meat proteins) |
| SAMN46298675 | diet_detail | PulD: pulses-enriched diet (surrogating red meat proteins) | R1 | 0.90 | biosample.attribute:special_diet_details | PulD: pulses-enriched diet (surrogating red meat proteins) |
| SAMN46298609 | health_condition | healthy_control | R4 | 0.50 | study.description | Volunteers participating to the trial |
| SAMN46298609 | health_condition_detail | Dietary intervention: pulses-enriched diet, pulses + plant-based meat surrogates, or habitual diet | R4 | 0.50 | study.description | Volunteers participating to the trial |
| SAMN46298609 | intervention | no_intervention | R1 | 0.80 | biosample.attribute:special_diet_details | special_diet_details: HabD: control diet |
| SAMN46298609 | intervention_detail | habitual control diet | R1 | 0.80 | biosample.attribute:special_diet_details | special_diet_details: HabD: control diet |
| SAMN46298609 | subject_id | P101 | R1 | 0.85 | sample.attr.subject_id | P101 |
| SAMN46298609 | timepoint_label | baseline | R1 | 0.85 | sample.attr.collection_time | baseline |
Sequencing
253 runs over 253 samples · mean 10.57 Gbp per run (median 10.58 Gbp, total 2,674.27 Gbp; 253 runs with a base count) · layout PAIRED 253 · platform ILLUMINA 253 · models Illumina NovaSeq 6000 (253) · 92% of runs with a Sandpiper profile · first public 2025-01-19 – 2025-03-13
Authors (0)
No author record.
Downloads
PRJNA1212336.csv.gz (8.4 KB) · parquet (81.5 KB) — all 253 samples, every field with route and confidence · PRJNA1212336_determinations.csv.gz (16.3 KB) — 2,362 evidence rows