Gut metagenomes from healthy adults, Guelph, Canada
Shotgun metagenomics of 4 faecal samples from healthy adults in Guelph, Canada, generated to study Lachnospiraceae urease activity and urea-carbon salvage into short-chain fatty acids in the human gut microbiota. machine-written summary
4 samples · 4 runs · CA · Adult · 2025 · 1.63 Gbp / sample
Checked this project's papers and archive records yourself? Mark it human-verified.
what each source gave
| ✓ Sequence archive | 28 sample values (collection date, country, latitude, location locality, location region, longitude, subject id) |
| ✓ Abstract / description | 8 sample values (health condition, health condition detail) |
| ✓ Full text | 4 sample values (location site) · PMC12001548 |
| ○ Supplementary tables | files read; no table could be matched to the samples |
| – External resources | not covered by the external resources we ingest |
| – User contribution | nothing uploaded yet |
| – Human verified | not yet checked by a person · I checked this project — verify it |
Genera, mean relative abundance over 3 profiled gut samples of this project (Sandpiper SingleM profiles, GTDB R232; low-depth and non-metagenome profiles left out).
| field | samples | coverage | R1 | R2 | R3 | R4 |
|---|---|---|---|---|---|---|
| age_category | 4 | 100% | ||||
| country | 4 | 100% | 4 | |||
| health_condition | 4 | 100% | 4 | |||
| subject_id | 4 | 100% | 4 | |||
| collection_date | 4 | 100% | 4 | |||
| detailed_location | 4 | 100% | ||||
| health_condition_detail | 4 | 100% | 4 | |||
| latitude | 4 | 100% | 4 | |||
| location_locality | 4 | 100% | 4 | |||
| location_region | 4 | 100% | 4 | |||
| location_site | 4 | 100% | 4 | |||
| longitude | 4 | 100% | 4 |
- Urease in acetogenic <i>Lachnospiraceae</i> drives urea carbon salvage in SCFA pools. 2025 PMID 40231625 PMC12001548 own_data
Archive description
Shotgun sequencing of human gut microbiome.
How this project entered the registry
Classified as human gut / stool shotgun DNA metagenome of adult by deterministic_prior with confidence 0.90. Assay shotgun_dna.
- host external_curation.infant_catalog · “infant triage reason_code age_adult_only”
- body site study.title · “Human faecal sample shotgun metagenomics”
- body site study.description · “sequencing of human gut microbiome.”
- life stage external_curation.infant_catalog · “infant triage reason_code age_adult_only”
Evidence examples (one per field × route)
| sample | field | value | route | conf. | source | quote |
|---|---|---|---|---|---|---|
| SAMN46506639 | collection_date | 2024-01 | R1 | 0.90 | biosample.attribute:collection_date | 2024-01 |
| SAMN46506639 | country | CA | R1 | 0.90 | sample.attr.geo_loc_name | Canada: Guelph |
| SAMN46506639 | health_condition | healthy_control | R4 | 0.45 | paper.abstract | healthy individuals |
| SAMN46506639 | health_condition_detail | Healthy individuals; study of normal gut microbiota physiology. | R4 | 0.45 | paper.abstract | healthy individuals |
| SAMN46506639 | latitude | 43.5302 | R1 | 0.90 | biosample.attribute:lat_lon | 43.5302 N 80.2285 W |
| SAMN46506639 | location_locality | Guelph | R1 | 0.85 | biosample.attribute:geo_loc_name | Canada: Guelph |
| SAMN46506639 | location_region | Ontario | R1 | 0.60 | biosample.attribute:lat_lon | 43.5302 N 80.2285 W |
| SAMN46506639 | location_site | University of Guelph main campus | R3 | 0.70 | paper.fulltext.methods | posters placed around the University of Guelph main campus |
| SAMN46506639 | longitude | -80.2285 | R1 | 0.90 | biosample.attribute:lat_lon | 43.5302 N 80.2285 W |
| SAMN46506639 | subject_id | 003 | R1 | 0.70 | sample.name_rule:alias | UGSP003 |
Sequencing
4 runs over 4 samples · mean 1.65 Gbp per run (median 1.63 Gbp, total 6.61 Gbp; 4 runs with a base count) · layout PAIRED 4 · platform ILLUMINA 4 · models Illumina HiSeq X (4) · 75% of runs with a Sandpiper profile · first public 2025-03-02 – 2025-03-03
Authors (10)
Firth IJ first · Sim MAR · Fitzgerald BG · Moore AE · Pittao CR · Gianetto-Hill C · Hess S · Sweeney AR · Allen-Vercoe E · Sorbara MT last
Downloads
PRJNA1217924.csv.gz (1.1 KB) · parquet (70.8 KB) — all 4 samples, every field with route and confidence · PRJNA1217924_determinations.csv.gz (1.1 KB) — 40 evidence rows