Gut microbiome ENGase discovery in Nevada adults
18 fecal samples from healthy adults in the USA (Nevada), cross-sectional, used to identify and characterize novel GH18 endo-β-N-acetylglucosaminidases acting on oligomannosidic N-glycans. machine-written summary
18 samples · 18 runs · US · Age unknown · 2025 · 7.29 Gbp / sample
Checked this project's papers and archive records yourself? Mark it human-verified.
what each source gave
| ✓ Sequence archive | 90 sample values (collection date, country, latitude, location region, longitude) |
| ○ Abstract / description | read; no usable statement |
| ✓ Full text | 36 sample values (health condition, health condition detail) · PMC12025955 |
| ○ Supplementary tables | files read; no table could be matched to the samples |
| – External resources | not covered by the external resources we ingest |
| – User contribution | nothing uploaded yet |
| – Human verified | not yet checked by a person · I checked this project — verify it |
Genera, mean relative abundance over 16 profiled gut samples of this project (Sandpiper SingleM profiles, GTDB R232; low-depth and non-metagenome profiles left out).
| field | samples | coverage | R1 | R2 | R3 | R4 |
|---|---|---|---|---|---|---|
| age_category | 18 | 100% | ||||
| country | 18 | 100% | 18 | |||
| health_condition | 18 | 100% | 18 | |||
| subject_id | 0 | 0% | ||||
| collection_date | 18 | 100% | 18 | |||
| detailed_location | 18 | 100% | ||||
| health_condition_detail | 18 | 100% | 18 | |||
| latitude | 18 | 100% | 18 | |||
| location_region | 18 | 100% | 18 | |||
| longitude | 18 | 100% | 18 |
- Novel Endo-β-<i>N</i>-Acetylglucosaminidases Derived from Human Fecal Samples Selectively Release <i>N</i>-Glycans from Model Glycoproteins. 2025 PMID 40282690 PMC12025955 own_data
Archive description
The primary purpose of this study is to collect fecal samples from a broadly representative population of the general population in Nevada to examine the interactions of diet, pharmaceutical intake, lifestyle, and the gut microbiome.
How this project entered the registry
Classified as human gut / stool shotgun DNA metagenome of age unknown by sonnet_x2 with confidence 0.70. Assay shotgun_dna.
- host run.host_tax_id · “9606”
- body site study.description · “collect fecal samples from a broadly representative population”
Evidence examples (one per field × route)
| sample | field | value | route | conf. | source | quote |
|---|---|---|---|---|---|---|
| SAMN46547975 | collection_date | 2022 | R1 | 0.90 | biosample.attribute:collection_date | 2022 |
| SAMN46547975 | country | US | R1 | 0.90 | sample.attr.geo_loc_name | USA |
| SAMN46547975 | health_condition | healthy_control | R3 | 0.70 | paper.fulltext.methods | no known and untreated infection |
| SAMN46547975 | health_condition_detail | no known and untreated infection; ad libitum diet without medical intervention | R3 | 0.70 | paper.fulltext.methods | no known and untreated infection |
| SAMN46547975 | latitude | 39.5299 | R1 | 0.90 | biosample.attribute:lat_lon | 39.5299 N 119.8143 W |
| SAMN46547975 | location_region | Nevada | R1 | 0.60 | biosample.attribute:lat_lon | 39.5299 N 119.8143 W |
| SAMN46547975 | longitude | -119.8143 | R1 | 0.90 | biosample.attribute:lat_lon | 39.5299 N 119.8143 W |
Sequencing
18 runs over 18 samples · mean 7.70 Gbp per run (median 7.29 Gbp, total 138.57 Gbp; 18 runs with a base count) · layout PAIRED 18 · platform ILLUMINA 18 · models Illumina HiSeq 2000 (18) · 89% of runs with a Sandpiper profile · first public 2025-02-06
Authors (7)
Bolino M first · Haththotuwe Gamage N · Duman H · Abiodun O · De Mello AS · Karav S · Frese SA last
Downloads
PRJNA1219209.csv.gz (1.4 KB) · parquet (70.5 KB) — all 18 samples, every field with route and confidence · PRJNA1219209_determinations.csv.gz (1.4 KB) — 126 evidence rows