Ex vivo gut fermentation testing glycans for GABA production
63 fecal microbiota samples from healthy controls, used in an ex vivo intervention study testing abiotic glycans (prebiotic/fibre) for effects on gut microbial GABA production. machine-written summary
63 samples · 63 runs · Adult · 2025 · 89.0 Mbp / sample
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what each source gave
| ✓ Sequence archive | 63 sample values (collection date) |
| ✓ Abstract / description | 126 sample values (health condition, health condition detail) |
| – Full text | no open-access full text linked |
| – Supplementary tables | no supplementary tables available (or none open access) |
| – External resources | not covered by the external resources we ingest |
| – User contribution | nothing uploaded yet · share metadata |
| – Human verified | not yet checked by a person · I checked this project — verify it |
intervention — arms not resolved
Prebiotic / dietary fibre supplement design observational_with_procedure · population healthy_control · Abiotic glycan SG-Glc added to ex vivo fecal fermentation
“impact of abiotic oligosaccharides on human fecal microbiota” — bioproject.description (study level; per-sample arms are in the intervention column below)
No Sandpiper profile for this project's samples yet.
| field | samples | coverage | R1 | R2 | R3 | R4 |
|---|---|---|---|---|---|---|
| age_category | 0 | 0% | ||||
| country | 0 | 0% | ||||
| health_condition | 63 | 100% | 63 | |||
| subject_id | 0 | 0% | ||||
| collection_date | 63 | 100% | 63 | |||
| health_condition_detail | 63 | 100% | 63 |
Archive description
Background: Prebiotics, substrates selectively utilized by host microorganisms to confer health benefits, can induce the production of microbial metabolites involved in regulating host biological functions. Several of these metabolites, including gamma-aminobutyric acid (GABA), play a crucial role in regulating neural activity and can be linked to a variety of neurological as well as physiological disorders. While GABA is primarily synthesized in the brain, emerging evidence suggests that the gut microbiome can also contribute to systemic GABA levels. Abiotic glycans, synthesized oligosaccharides containing structures not typically found in nature, are a promising candidate for increasing systemic GABA levels through the targeted modulation of the gut microbiome via the enrichment of GABA-producing microorganisms.</p><p>Results: In this study, we investigated the impact of abiotic oligosaccharides on human fecal microbiota and GABA production. We identified a specific glycan, SG-Glc, that significantly increases GABA levels in ex vivo fermentation experiments wherein the constituent simple sugar had no impact on GABA levels. We find Bifidobacterium adolescentis is a key species driving this increase. Further analysis revealed that B. adolescentis possesses both genes required for GABA production and a unique CAZyme (carbohydrate-active enzyme) profile enabling it to utilize SG-Glc for growth while producing GABA, unlike other Bifidobacterium species.</p><p>Conclusions: Our findings highlight the potential of abiotic oligosaccharides to modulate the gut microbiome and enhance GABA production, offering an expanded toolbox for targeting the gut-brain axis. By understanding the specific mechanisms underlying these effects, we may develop innovative strategies for improving overall brain function with possible impacts on both mental and physiological health and well-being.
How this project entered the registry
Classified as human gut / stool shotgun DNA metagenome of adult and adolescent by deterministic_prior with confidence 0.90. Assay shotgun_dna.
- host external_curation.infant_catalog · “infant triage reason_code age_adult_only”
- body site study.title · “Production in Human Gut Model”
- body site study.description · “suggests that the gut microbiome can also”
- life stage external_curation.infant_catalog · “infant triage reason_code age_adult_only”
- life stage study.description · “We find Bifidobacterium adolescentis is a key”
Evidence examples (one per field × route)
| sample | field | value | route | conf. | source | quote |
|---|---|---|---|---|---|---|
| SAMN48083452 | collection_date | 2024-03-27 | R1 | 0.90 | biosample.attribute:collection_date | 2024-03-27 |
| SAMN48083452 | health_condition | healthy_control | R4 | 0.50 | study.description | investigated the impact of abiotic oligosaccharides on human fecal microbiota |
| SAMN48083452 | health_condition_detail | ex vivo fermentation with abiotic oligosaccharides (prebiotic intervention) | R4 | 0.50 | study.description | investigated the impact of abiotic oligosaccharides on human fecal microbiota |
Sequencing
63 runs over 63 samples · mean 0.11 Gbp per run (median 89.0 Mbp, total 7.12 Gbp; 63 runs with a base count) · layout SINGLE 63 · platform OXFORD_NANOPORE 63 · models GridION (63) · 0% of runs with a Sandpiper profile · first public 2025-04-23 – 2025-04-27
Authors (0)
No author record.
Help complete this project
3 of the 4 core fields are below 50% coverage: age_category, country, subject_id. Share metadata
Downloads
PRJNA1225188.csv.gz (2.3 KB) · parquet (71.1 KB) — all 63 samples, every field with route and confidence · PRJNA1225188_determinations.csv.gz (1.5 KB) — 189 evidence rows