Resistant starch intervention in MASLD, China
55 gut metagenome samples from adults with MASLD in Shanghai and Hainan, China, from a multicenter, single-arm resistant starch intervention trial. machine-written summary
55 samples · 55 runs · CN · Adult · 2025 · 6.71 Gbp / sample
Checked this project's papers and archive records yourself? Mark it human-verified.
what each source gave
| ✓ Sequence archive | 308 sample values (collection date, country, latitude, location locality, location region, longitude) |
| ✓ Abstract / description | 110 sample values (health condition, health condition detail) |
| – Full text | no open-access full text linked |
| – Supplementary tables | no supplementary tables available (or none open access) |
| – External resources | not covered by the external resources we ingest |
| – User contribution | nothing uploaded yet |
| – Human verified | not yet checked by a person · I checked this project — verify it |
intervention — arms not resolved
design single_arm_before_after
Genera, mean relative abundance over 55 profiled gut samples of this project (Sandpiper SingleM profiles, GTDB R232; low-depth and non-metagenome profiles left out).
| field | samples | coverage | R1 | R2 | R3 | R4 |
|---|---|---|---|---|---|---|
| age_category | 55 | 100% | ||||
| country | 55 | 100% | 55 | |||
| health_condition | 55 | 100% | 55 | |||
| subject_id | 0 | 0% | ||||
| collection_date | 55 | 100% | 55 | |||
| detailed_location | 55 | 100% | ||||
| health_condition_detail | 55 | 100% | 55 | |||
| latitude | 55 | 100% | 55 | |||
| longitude | 55 | 100% | 55 | |||
| location_locality | 48 | 87% | 48 | |||
| location_region | 40 | 73% | 40 |
- Interindividual variability in gut microbiome mediates the efficacy of resistant starch on MASLD. 2025 PMID 41270737 own_data
Archive description
Metabolic dysfunction-associated steatotic liver disease (MASLD) is affecting over 30% of adults globally. But effective treatment options remain limited. Resistant starch (RS) have been found to reduce intrahepatic triglyceride content (IHTC), offering a novel therapeutic approach. However, there is heterogeneity in treatment responses, with some patients showing marked improvements in IHTC while others did not, which limits therapeutic application of RS. Therefore, to explore the underlying causes of the heterogeneity and identify strategies to extend benefits to a broader cohort of people with MASLD, we conducted a multicenter, single-arm RS intervention trial. By integrating shotgun metagenomic sequencing and changes of clinical indicators, we characterised the baseline gut microbiota composition, especially two keystone bacteria, as a driver of the heterogeneity.
How this project entered the registry
Classified as human gut / stool shotgun DNA metagenome of adult by sonnet_x2 with confidence 0.70. Assay shotgun_dna.
- host run.host_tax_id · “9606”
- host run.scientific_name · “gut metagenome”
- body site study.description · “baseline gut microbiota composition”
- life stage study.description · “affecting over 30% of adults globally”
Evidence examples (one per field × route)
| sample | field | value | route | conf. | source | quote |
|---|---|---|---|---|---|---|
| SAMN47305078 | collection_date | 2023-08-22 | R1 | 0.90 | biosample.attribute:collection_date | 2023-08-22 |
| SAMN47305078 | country | CN | R1 | 0.90 | sample.attr.geo_loc_name | China: Shanghai |
| SAMN47305078 | health_condition | liver_disease | R4 | 0.50 | paper.abstract | metabolic dysfunction-associated steatotic liver disease (MASLD) |
| SAMN47305078 | health_condition_detail | metabolic dysfunction-associated steatotic liver disease (MASLD) | R4 | 0.50 | paper.abstract | metabolic dysfunction-associated steatotic liver disease (MASLD) |
| SAMN47305078 | latitude | 31.1800 | R1 | 0.90 | biosample.attribute:lat_lon | 31.18 N 121.42 E |
| SAMN47305078 | location_locality | Shanghai | R1 | 0.85 | biosample.attribute:geo_loc_name | China: Shanghai |
| SAMN47305078 | location_region | Shanghai Shi | R1 | 0.60 | biosample.attribute:lat_lon | 31.18 N 121.42 E |
| SAMN47305126 | location_region | Hainan | R1 | 0.85 | biosample.attribute:geo_loc_name | China: Hainan |
| SAMN47305078 | longitude | 121.4200 | R1 | 0.90 | biosample.attribute:lat_lon | 31.18 N 121.42 E |
Sequencing
55 runs over 55 samples · mean 6.68 Gbp per run (median 6.71 Gbp, total 367.50 Gbp; 55 runs with a base count) · layout PAIRED 55 · platform ILLUMINA 55 · models Illumina NovaSeq 6000 (55) · 100% of runs with a Sandpiper profile · first public 2025-10-23
Authors (0)
No author record.
Downloads
PRJNA1234627.csv.gz (2.5 KB) · parquet (72.5 KB) — all 55 samples, every field with route and confidence · PRJNA1234627_determinations.csv.gz (4.0 KB) — 418 evidence rows