Fecal virome method comparison in healthy adults, Galicia
18 fecal samples from healthy adults in Galicia, Spain, used to compare viral enrichment, extraction, amplification, and library preparation protocols for recovering the human gut virome. machine-written summary
18 samples · 49 runs · ES · Age unknown · 2026 · 0.90 Gbp / sample
Checked this project's papers and archive records yourself? Mark it human-verified.
what each source gave
| ✓ Sequence archive | 54 sample values (collection date, country, location region) |
| ○ Abstract / description | read; no usable statement |
| ✓ Full text | 72 sample values (antibiotic exposure, health condition, health condition detail, location site) · PMC13155102 |
| ○ Supplementary tables | files read; no table could be matched to the samples |
| – External resources | not covered by the external resources we ingest |
| – User contribution | nothing uploaded yet |
| – Human verified | not yet checked by a person · I checked this project — verify it |
No Sandpiper profile for this project's samples yet.
| field | samples | coverage | R1 | R2 | R3 | R4 |
|---|---|---|---|---|---|---|
| age_category | 18 | 100% | ||||
| country | 18 | 100% | 18 | |||
| health_condition | 18 | 100% | 18 | |||
| subject_id | 0 | 0% | ||||
| antibiotic_exposure | 18 | 100% | 18 | |||
| collection_date | 18 | 100% | 18 | |||
| detailed_location | 18 | 100% | ||||
| health_condition_detail | 18 | 100% | 18 | |||
| location_region | 18 | 100% | 18 | |||
| location_site | 18 | 100% | 18 |
- A comparison of methods for the optimal recovery of the human fecal virome. 2026 PMID 42111296 PMC13155102 own_data
Archive description
We evaluated different protocols for shotgun metagenomic sequencing of the human fecal virome. Human fecal samples were processed using multiple strategies involving viral enrichment, nucleic acid extraction, amplification, and library preparation methods. The resulting libraries were sequenced and compared to assess protocol-specific impacts on taxonomic and functional assignment, contig quality, viral diversity, and genome structure.
How this project entered the registry
Classified as human gut / stool shotgun DNA metagenome of age unknown by sonnet_x2 with confidence 0.68. Assay shotgun_dna.
- host run.host_tax_id · “9606”
- body site study.title · “human fecal virome”
Evidence examples (one per field × route)
| sample | field | value | route | conf. | source | quote |
|---|---|---|---|---|---|---|
| SAMN47935459 | antibiotic_exposure | no | R3 | 0.70 | paper.fulltext.methods | not taken antibiotics for at least 2 months |
| SAMN47935459 | collection_date | 2019-03-29 | R1 | 0.90 | biosample.attribute:collection_date | 2019-03-29 |
| SAMN47935459 | country | ES | R1 | 0.90 | sample.attr.geo_loc_name | Spain: Galicia |
| SAMN47935459 | health_condition | healthy_control | R3 | 0.70 | paper.fulltext.methods | 18 adult volunteers who had not taken antibiotics |
| SAMN47935459 | health_condition_detail | adult volunteers | R3 | 0.70 | paper.fulltext.methods | 18 adult volunteers who had not taken antibiotics |
| SAMN47935459 | location_region | Galicia | R1 | 0.85 | biosample.attribute:geo_loc_name | Spain: Galicia |
| SAMN47935459 | location_site | Complexo Hospitalario Universitario de Ourense | R3 | 0.70 | paper.fulltext.methods | recruited at the Complexo Hospitalario Universitario de Ourense |
Sequencing
49 runs over 18 samples · mean 0.30 Gbp per run (median 0.23 Gbp, total 14.94 Gbp; 49 runs with a base count) · layout PAIRED 44, SINGLE 5 · platform ILLUMINA 49 · models Illumina MiniSeq (49) · 0% of runs with a Sandpiper profile · first public 2026-04-15
Authors (10)
De Chiara L first · Doughty R · Estévez-Gómez N · Gallego-García P · Alvariño P · Díez-Martín A · Dávila Piñón P · Treangen TJ · Cubiella J · Posada D last
Downloads
PRJNA1250239.csv.gz (1.4 KB) · parquet (70.7 KB) — all 18 samples, every field with route and confidence · PRJNA1250239_determinations.csv.gz (1.6 KB) — 126 evidence rows