Multi-donor FMT trial in anorexia nervosa, Auckland NZ
98 stool samples from an open-label feasibility pilot trial of encapsulated, multi-donor faecal microbiota transfer in young women with anorexia nervosa in Auckland, New Zealand. machine-written summary
98 samples · 98 runs · NZ · Adult · 2025 · 5.97 Gbp / sample
Checked this project's papers and archive records yourself? Mark it human-verified.
what each source gave
| ✓ Sequence archive | 613 sample values (collection date, country, intervention, intervention detail, location locality, subject id, timepoint) |
| ✓ Abstract / description | 196 sample values (health condition, health condition detail) |
| ✓ Full text | 196 sample values (location site, sex) · PMC12808231 |
| ○ Supplementary tables | files read; no table could be matched to the samples |
| – External resources | not covered by the external resources we ingest |
| – User contribution | nothing uploaded yet |
| – Human verified | not yet checked by a person · I checked this project — verify it |
intervention · arms resolved
Faecal microbiota transplantation design single_arm_before_after · population other_disease · Encapsulated multi-donor faecal microbiota transfer, 20 capsules/4 donors
“capsule-delivered, multi-donor FMT intervention” — bioproject.description (study level; per-sample arms are in the intervention column below)
longitudinalFMT donor → recipient — not resolved
26 of 37 people sampled more than once (87 samples; median 3, up to 5 per person) · follow-up median 3 mo (max 11 mo) · median interval between samples 23 d (time from collection dates). Schedule stated by the source: “Stool collected at enrolment (3wk pre-treatment), baseline, and 3, 6, 12 weeks post-treatment.”.
Each row is one person (26 shown); dots are samples at days since that person's first sample.
18 AN participants received capsules from a shared pool of multiple stool donors (multi-donor FMT); donor samples also included..
Genera, mean relative abundance over 98 profiled gut samples of this project (Sandpiper SingleM profiles, GTDB R232; low-depth and non-metagenome profiles left out).
| field | samples | coverage | R1 | R2 | R3 | R4 |
|---|---|---|---|---|---|---|
| age_category | 98 | 100% | ||||
| country | 98 | 100% | 98 | |||
| health_condition | 98 | 100% | 98 | |||
| subject_id | 98 | 100% | 98 | |||
| collection_date | 98 | 100% | 98 | |||
| detailed_location | 98 | 100% | ||||
| health_condition_detail | 98 | 100% | 98 | |||
| location_locality | 98 | 100% | 98 | |||
| location_site | 98 | 100% | 98 | |||
| sex | 98 | 100% | 98 | |||
| intervention | 92 | 94% | 92 | |||
| timepoint_label | 66 | 67% | 66 | |||
| intervention_detail | 63 | 64% | 63 |
- Encapsulated faecal microbiota transfer in young women with anorexia nervosa: an open-label feasibility pilot trial. 2025 PMID 41392089 PMC12808231 own_data
Archive description
The Gut Bugs in Anorexia Nervosa trial was a one-arm, open-label feasibility pilot trial evaluated the tolerability and microbiological impact of encapsulated, multi-donor FMT in young women with AN (Registration: ACTRN12621001504808). The study was conducted in Auckland, New Zealand, between September 2022 and February 2024.</p><p>Eighteen participants were enrolled in a capsule-delivered, multi-donor FMT intervention, with 15 completing treatment. Each participant received 20 capsules containing concentrated gut microbiota from four different donors (5 capsules per donor), taken over one or two consecutive days. No bowel cleanse was performed prior to FMT for safety reasons. Given the 18 month recruitment phase, three separate FMT treatment batches were prepared with different combinations of donor microbiota.</p><p>Stool samples were collected at five time points: at enrolment (3 weeks before treatment), baseline (when treatment was administered), and 3, 6, and 12 weeks post-treatment. Stool samples were preserved in room-temperature-stable DNA/RNA Shield Fecal Collection Tubes (R1101, Zymo Research, Irvine, California, U.S.) before transfer to -80C for long-term storage. For donors, pre- and post-processing stool samples were collected at each donation and stored at -80C in DNA/RNA Shield.
How this project entered the registry
Classified as human gut / stool shotgun DNA metagenome of adult by deterministic_prior with confidence 0.90. Assay shotgun_dna.
- host external_curation.infant_catalog · “infant triage reason_code age_adult_only”
- body site study.title · “Gut Bugs in Anorexia”
- body site study.description · “The Gut Bugs in Anorexia”
- life stage external_curation.infant_catalog · “infant triage reason_code age_adult_only”
- life stage study.description · “FMT in young women with AN (Registration:”
Evidence examples (one per field × route)
| sample | field | value | route | conf. | source | quote |
|---|---|---|---|---|---|---|
| SAMN48745291 | collection_date | 2022-08-31 | R1 | 0.90 | biosample.attribute:collection_date | 2022-08-31 |
| SAMN48745291 | country | NZ | R1 | 0.90 | sample.attr.geo_loc_name | New Zealand: Auckland |
| SAMN48745291 | health_condition | other_disease | R4 | 0.50 | study.description | young women with AN |
| SAMN48745291 | health_condition_detail | anorexia nervosa | R4 | 0.50 | study.description | young women with AN |
| SAMN48745291 | intervention | fmt | R1 | 0.90 | biosample.attribute:completed_fmt | completed_fmt: Yes |
| SAMN48745357 | intervention | no_intervention | R1 | 0.70 | biosample.attribute:participant_type | Donor |
| SAMN48745291 | intervention_detail | completed full course of FMT capsules | R1 | 0.90 | biosample.attribute:completed_fmt | completed_fmt: Yes |
| SAMN48745291 | location_locality | Auckland | R1 | 0.85 | biosample.attribute:geo_loc_name | New Zealand: Auckland |
| SAMN48745291 | location_site | Liggins Institute Clinical Research Unit, University of Auckland | R3 | 0.70 | paper.fulltext.methods | carried out at the Liggins Institute Clinical Research Unit |
| SAMN48745291 | sex | female | R3 | 0.70 | paper.fulltext.methods | eligible participants were biological females aged |
| SAMN48745291 | subject_id | AN-F01 | R1 | 0.85 | sample.attr.participant_id | AN-F01 |
| SAMN48745291 | timepoint_label | EN | R1 | 0.70 | biosample.attribute:sample_type | EN |
Sequencing
98 runs over 98 samples · mean 6.08 Gbp per run (median 5.97 Gbp, total 595.86 Gbp; 98 runs with a base count) · layout PAIRED 98 · platform ILLUMINA 98 · models Illumina NovaSeq 6000 (98) · 100% of runs with a Sandpiper profile · first public 2025-12-22
Authors (12)
Wilson BC first · Tweedie-Cullen RY · Albert BB · Derraik JGB · Ho D · Depczynski M · Creagh C · Edwards T · Gali S · Thabrew H · Cutfield WS · O'Sullivan JM last
Downloads
PRJNA1268178.csv.gz (4.3 KB) · parquet (76.2 KB) — all 98 samples, every field with route and confidence · PRJNA1268178_determinations.csv.gz (7.5 KB) — 1,005 evidence rows