Multi-site microbiome in multiple primary lung cancer, China
176 samples from 21 MPLC patients across seven sampling sites (oral, upper GI, lung tissue, respiratory tract) in Beijing, China, profiled by full-length 16S rRNA gene sequencing to examine gastrointestinal-pulmonary microbial associations. machine-written summary
176 samples · 176 runs · CN · Age unknown · 2025 · 0.24 Gbp / sample
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what each source gave
| ✓ Sequence archive | 528 sample values (collection date, country, location locality) |
| ✓ Abstract / description | 352 sample values (health condition, health condition detail) |
| – Full text | no open-access full text linked |
| – Supplementary tables | no supplementary tables available (or none open access) |
| – External resources | not covered by the external resources we ingest |
| – User contribution | nothing uploaded yet · share metadata |
| – Human verified | not yet checked by a person · I checked this project — verify it |
No Sandpiper profile for this project's samples yet.
| field | samples | coverage | R1 | R2 | R3 | R4 |
|---|---|---|---|---|---|---|
| age_category | 0 | 0% | ||||
| country | 176 | 100% | 176 | |||
| health_condition | 176 | 100% | 176 | |||
| subject_id | 0 | 0% | ||||
| collection_date | 176 | 100% | 176 | |||
| detailed_location | 176 | 100% | ||||
| health_condition_detail | 176 | 100% | 176 | |||
| location_locality | 176 | 100% | 176 |
Archive description
Multiple primary lung cancer (MPLC) is a distinct subtype of lung cancer characterized by the presence of two or more independent lesions within the lungs. Increasing evidence suggests that microbial dysbiosis may play a role in lung carcinogenesis however, the microbial characteristics associated with MPLC development remain unclear. In this study, we performed full-length 16S rRNA gene sequencing on 176 samples collected from 21 MPLC patients across seven sampling sites. Bacterial diversity and community composition differed significantly across sampling sites. The oral cavity and upper gastrointestinal tract (UGI) exhibited the highest richness and a similar microbial profile, while lung tissue and respiratory tract samples harbored less diverse but compositionally related communities. Microbial source tracking revealed that a subset of bacteria in pulmonary nodules originated from the UGI tract, suggesting possible microbial translocation associated with gastroesophageal reflux. Phylogenetic analysis further identified two species, Prevotella melaninogenica and Gemella haemolysans, as the most frequently shared taxa in the lung and UGI microbiota of MPLC patients, potentially contributing to tumorigenesis. Furthermore, analysis of the lung microbiota revealed microbial heterogeneity among pulmonary nodules in MPLC patients, underscoring the need to analyze each nodule independently. This study provides a comprehensive characterization of the microbiota in MPLC patients and highlights potential microbial links between the gastrointestinal and pulmonary systems.
How this project entered the registry
Classified as human oral cavity / gut / stool / lower respiratory / multiple sites / nasal / nasopharyngeal shotgun DNA metagenome of age unknown by sonnet_x2 with confidence 0.55. Assay shotgun_dna.
- host run.host_tax_id · “9606”
- body site study.description · “oral cavity and upper gastrointestinal tract”
- body site study.description · “lung tissue and respiratory tract samples”
- body site sample.attr.env_broad_scale · “URT (n=19/176)”
Evidence examples (one per field × route)
| sample | field | value | route | conf. | source | quote |
|---|---|---|---|---|---|---|
| SAMN49669334 | collection_date | 2023 | R1 | 0.90 | biosample.attribute:collection_date | 2023 |
| SAMN49669334 | country | CN | R1 | 0.90 | sample.attr.geo_loc_name | China: Beijing |
| SAMN49669334 | health_condition | other_cancer | R4 | 0.50 | study.description | MPLC patients across seven sampling sites |
| SAMN49669334 | health_condition_detail | Multiple primary lung cancer | R4 | 0.50 | study.description | MPLC patients across seven sampling sites |
| SAMN49669334 | location_locality | Beijing | R1 | 0.85 | biosample.attribute:geo_loc_name | China: Beijing |
Sequencing
176 runs over 176 samples · mean 0.24 Gbp per run (median 0.24 Gbp, total 42.91 Gbp; 176 runs with a base count) · layout SINGLE 176 · platform PACBIO_SMRT 176 · models Sequel II (176) · 0% of runs with a Sandpiper profile · first public 2025-08-26
Authors (0)
No author record.
Help complete this project
2 of the 4 core fields are below 50% coverage: age_category, subject_id. Share metadata
Downloads
PRJNA1282887.csv.gz (4.8 KB) · parquet (75.8 KB) — all 176 samples, every field with route and confidence · PRJNA1282887_determinations.csv.gz (5.0 KB) — 880 evidence rows