Fecal 16S survey of MASLD patients, Durham NC
55 fecal samples from biopsy-proven MASLD patients in Durham, NC, examined in a cross-sectional study of bile salt hydrolase activity and gut bacterial contributions to MASLD. machine-written summary
55 samples · 55 runs · US · Adult · 2025 · 68.2 Mbp / sample
Checked this project's papers and archive records yourself? Mark it human-verified.
what each source gave
| ✓ Sequence archive | 604 sample values (age, collection date, country, health condition, health condition detail, latitude, location locality, location region) |
| ○ Abstract / description | read; no usable statement |
| ○ Full text | read; no usable statement or table · PMC12773562 |
| ○ Supplementary tables | files read; no table could be matched to the samples |
| – External resources | not covered by the external resources we ingest |
| – User contribution | nothing uploaded yet |
| – Human verified | not yet checked by a person · I checked this project — verify it |
intervention — arms not resolved
design observational_with_procedure
age: two sequence archives disagree for 1 sample (e.g. 76.0 vs 27919.7) — withheld.
The sources disagree on 1 sample. Where two incompatible values exist for one sample (or one person's samples disagree on sex), neither value is published: the field is left empty here, in the sample sheet and in the downloads. Both sides, with their sources, are in gut_metadata_contradictions.parquet of the data package (what counts as a contradiction).
Contradicted values (1)
| sample | field | value | other value | handling |
|---|---|---|---|---|
| SAMN50097766 | age | 76.0 (sequence archive) | 27919.7 (sequence archive) | withheld: neither value published |
No Sandpiper profile for this project's samples yet.
| field | samples | coverage | R1 | R2 | R3 | R4 |
|---|---|---|---|---|---|---|
| age_category | 55 | 100% | ||||
| country | 55 | 100% | 55 | |||
| health_condition | 55 | 100% | 55 | |||
| subject_id | 55 | 100% | 55 | |||
| collection_date | 55 | 100% | 55 | |||
| detailed_location | 55 | 100% | ||||
| health_condition_detail | 55 | 100% | 55 | |||
| latitude | 55 | 100% | 55 | |||
| location_locality | 55 | 100% | 55 | |||
| location_region | 55 | 100% | 55 | |||
| longitude | 55 | 100% | 55 | |||
| sex | 55 | 100% | 55 | |||
| age_at_collection_days | 54 | 98% | 54 |
- Bile salt hydrolase activity as a rational target for MASLD therapy. 2026 PMID 41481156 PMC12773562 own_data
Archive description
Fecal samples collected from biopsy-proven MASLD patients
How this project entered the registry
Classified as human gut / stool shotgun DNA metagenome of adult and elderly by sonnet_x2 with confidence 0.62. Assay shotgun_dna.
- host run.scientific_name · “human feces metagenome”
- body site study.description · “Fecal samples collected from biopsy-proven MASLD”
- life stage sample.attr.host_age · “21.57 (n=38/55)”
- life stage sample.attr.host_age · “61.89 (n=16/55)”
Evidence examples (one per field × route)
| sample | field | value | route | conf. | source | quote |
|---|---|---|---|---|---|---|
| SAMN50097758 | age_at_collection_days | 19398.0 | R1 | 0.85 | sample.attr.host_age | 53.16 |
| SAMN50097758 | collection_date | 2019/2020 | R1 | 0.90 | biosample.attribute:collection_date | 2019/2020 |
| SAMN50097758 | country | US | R1 | 0.90 | sample.attr.geo_loc_name | USA: Durham, NC |
| SAMN50097758 | health_condition | cardiometabolic_other | R1 | 0.90 | biosample_attr | MASLD |
| SAMN50097758 | health_condition_detail | MASLD | R1 | 0.90 | sample.attr.host_disease | MASLD |
| SAMN50097758 | latitude | 36.0000 | R1 | 0.90 | biosample.attribute:lat_lon | 36.00 N 78.56 W |
| SAMN50097758 | location_locality | Durham | R1 | 0.85 | biosample.attribute:geo_loc_name | USA: Durham, NC |
| SAMN50097758 | location_region | North Carolina | R1 | 0.75 | biosample.attribute:geo_loc_name | USA: Durham, NC |
| SAMN50097758 | longitude | -78.5600 | R1 | 0.90 | biosample.attribute:lat_lon | 36.00 N 78.56 W |
| SAMN50097758 | sex | female | R1 | 0.90 | sample.attr.host_sex | female |
| SAMN50097758 | subject_id | MASLD_patient01 | R1 | 0.70 | sample.name_rule:alias | MASLD_patient01 |
Sequencing
55 runs over 55 samples · mean 68.5 Mbp per run (median 68.2 Mbp, total 3.77 Gbp; 55 runs with a base count) · layout PAIRED 55 · platform ILLUMINA 55 · models Illumina MiSeq (55) · 0% of runs with a Sandpiper profile · first public 2025-07-24
Authors (14)
Jones EV first · Wang Y · Wei W · Reed JC · Chaudhari SN · Li DK · Boursier J · Lang S · Demir M · Diehl AM · Allegretti AS · Schnabl B · Chung RT · Devlin AS last
Downloads
PRJNA1294521.csv.gz (2.7 KB) · parquet (73.2 KB) — all 55 samples, every field with route and confidence · PRJNA1294521_determinations.csv.gz (5.3 KB) — 604 evidence rows