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PRJNA1308943 SRP610361

Stool metagenomes from US foodborne outbreaks (UnO project)

Metagenomic sequencing of stool from foodborne outbreaks for the Undetermined Outbreaks project (UnO)

51 stool samples from foodborne outbreak investigations across sites including Colorado, Alabama (Demopolis), Connecticut (Norwalk), and Utah, USA, sequenced to help identify novel or changed diarrheal pathogens of unknown etiology. machine-written summary

51 samples · 51 runs · US · Age unknown · 2026 · 4.67 Gbp / sample

Sources
✓Sequence archive 237○Abstract / description–Full text–Supplementary tables–External resources–User contribution–Human verified

Checked this project's papers and archive records yourself? Mark it human-verified.

what each source gave
✓ Sequence archive237 sample values (collection date, country, health condition, health condition detail, latitude, location locality, location region, longitude)
○ Abstract / descriptionread; no usable statement
– Full textno open-access full text linked
– Supplementary tablesno supplementary tables available (or none open access)
– External resourcesnot covered by the external resources we ingest
– User contributionnothing uploaded yet · share metadata
– Human verifiednot yet checked by a person · I checked this project — verify it
Samples at a glance
4 sampling locations · 1 country
Age
unknown51
Condition
gi_infection_or_diarrhoea31
Country
US35
Body site
primary51
Collected 2014–2024
2014: 4 samples2024: 31 samples20142024
Sequencing depth per sample · this project all projects
all projects, 0–0.03 Gbp: 25,080 samples (4 %)this project, 0–0.03 Gbp: 16 samplesall projects, 0.03–0.1 Gbp: 17,066 samples (3 %)this project, 0.03–0.1 Gbp: 1 samplesall projects, 0.1–0.3 Gbp: 29,179 samples (5 %)this project, 0.1–0.3 Gbp: 2 samplesall projects, 0.3–1 Gbp: 47,044 samples (8 %)this project, 0.3–1 Gbp: 1 samplesall projects, 1–3 Gbp: 124,983 samples (22 %)this project, 1–3 Gbp: 2 samplesall projects, 3–10 Gbp: 253,728 samples (44 %)this project, 3–10 Gbp: 22 samplesall projects, 10–30 Gbp: 72,533 samples (13 %)this project, 10–30 Gbp: 7 samplesall projects, 30–100 Gbp: 3,024 samples (1 %)all projects, 100–300 Gbp: 106 samples (0 %)all projects, ≥ 300 Gbp: 9 samples (0 %)project median 4.67 Gbp per sample0130Gbp
Community composition
Escherichia 21.9%Bacteroides 8.5%Bifidobacterium 7.4%Blautia_A 3.8%Phocaeicola 3.4%Faecalibacterium 3.3%Streptococcus 2.8%Mediterraneibacter 2.6%Alistipes 2.2%Enterococcus_B 2.0%Prevotella 1.9%Roseburia 1.7%not assigned to a genus 8.5%other genera 29.9%

Genera, mean relative abundance over 32 profiled gut samples of this project (Sandpiper SingleM profiles, GTDB R232; low-depth and non-metagenome profiles left out).

Field coverage
fieldsamplescoverageR1R2R3R4
age_category0 0%
country35 69%35
health_condition31 61%31
subject_id0 0%
collection_date35 69%35
detailed_location31 61%
health_condition_detail31 61%31
latitude31 61%31
location_region31 61%31
longitude31 61%31
location_locality12 24%12
Details
Archive description

The Undetermined Outbreaks project (UnO) is aimed at resolving foodborne outbreaks of unknown etiology using metagenomic sequencing. Our goal is to identify novel or changed diarrheal pathogens across outbreak stool sets with strong epidemiological links. These datasets include sequencing from foodborne outbreaks of known and unknown etiology collected by state, territorial, local, tribal, and other public health partners that were used in the development and validation of wet and dry lab tools for UnO.

How this project entered the registry

Classified as human gut / stool shotgun DNA metagenome of age unknown by sonnet_x2 with confidence 0.80. Assay shotgun_dna.

  • host run.scientific_name · “human gut metagenome”
  • host run.host_tax_id · “9606”
  • body site study.title · “Metagenomic sequencing of stool from foodborne outbreaks”
Evidence examples (one per field × route)
samplefieldvaluerouteconf.sourcequote
SAMN50715276collection_date2024-05-23R10.90biosample.attribute:collection_date2024-05-23
SAMN50715276countryUSR10.90sample.attr.geo_loc_nameUSA: Alabama, Demopolis
SAMN50715276health_conditiongi_infection_or_diarrhoeaR10.85biosample.attribute:descriptionStool metagenome from foodborne outbreak
SAMN50715276health_condition_detailStool metagenome from foodborne outbreakR10.85biosample.attribute:descriptionStool metagenome from foodborne outbreak
SAMN50715276latitude32.5100R10.90biosample.attribute:lat_lon32.51 N 87.83 W
SAMN50715276location_localityDemopolisR10.85biosample.attribute:geo_loc_nameUSA: Alabama, Demopolis
SAMN50715276location_regionAlabamaR10.85biosample.attribute:geo_loc_nameUSA: Alabama, Demopolis
SAMN50715276longitude-87.8300R10.90biosample.attribute:lat_lon32.51 N 87.83 W
Sequencing

51 runs over 51 samples · mean 4.23 Gbp per run (median 4.67 Gbp, total 215.80 Gbp; 51 runs with a base count) · layout PAIRED 51 · platform ILLUMINA 51 · models NextSeq 1000 (51) · 63% of runs with a Sandpiper profile · first public 2026-02-23

Authors (0)

No author record.

Help complete this project

2 of the 4 core fields are below 50% coverage: age_category, subject_id. Share metadata

Downloads

PRJNA1308943.csv.gz (2.2 KB) · parquet (71.9 KB) — all 51 samples, every field with route and confidence · PRJNA1308943_determinations.csv.gz (2.1 KB) — 237 evidence rows

Samples (51 · every field this project has values for · hover a cell for its source)
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