Gut microbiome in healthy Saudi adults by age, Riyadh
145 fecal samples from healthy Saudi adults aged 19-69 in Riyadh, profiled by shotgun metagenomic sequencing in a cross-sectional study to examine age-related variation in gut microbiota composition and diversity. machine-written summary
145 samples · 145 runs · SA · Adult · 2025 · 2.16 Gbp / sample
Checked this project's papers and archive records yourself? Mark it human-verified.
what each source gave
| ✓ Sequence archive | 725 sample values (age, collection date, country, location locality, sex) |
| ✓ Abstract / description | 145 sample values (health condition detail) |
| ✓ Full text | 145 sample values (health condition) · PMC13013359 |
| ○ Supplementary tables | files read; no table could be matched to the samples |
| – External resources | not covered by the external resources we ingest |
| – User contribution | nothing uploaded yet |
| – Human verified | not yet checked by a person · I checked this project — verify it |
Genera, mean relative abundance over 145 profiled gut samples of this project (Sandpiper SingleM profiles, GTDB R232; low-depth and non-metagenome profiles left out).
| field | samples | coverage | R1 | R2 | R3 | R4 |
|---|---|---|---|---|---|---|
| age_category | 145 | 100% | ||||
| country | 145 | 100% | 145 | |||
| health_condition | 145 | 100% | 145 | |||
| subject_id | 0 | 0% | ||||
| age_at_collection_days | 145 | 100% | 145 | |||
| collection_date | 145 | 100% | 145 | |||
| detailed_location | 145 | 100% | ||||
| health_condition_detail | 145 | 100% | 145 | |||
| location_locality | 145 | 100% | 145 | |||
| sex | 145 | 100% | 145 |
- First metagenomic analysis of age-associated changes in the gut microbiome among healthy Saudi adults: SAMS pilot study. 2026 PMID 41889698 PMC13013359 own_data
Archive description
This study investigates the composition of the fecal microbiome in healthy Saudi adults aged 19 to 69 years using shotgun metagenomic sequencing. Fecal samples were collected from various locations in Riyadh between January 2024 to February 2025. DNA was extracted from these samples and sequenced on the NextSeq 200 platform. The resulting sequences provide insights into variations in microbial diversity and community structure associated with healthy aging.
How this project entered the registry
Classified as human gut / stool shotgun DNA metagenome of adult by sonnet_x2 with confidence 0.72. Assay shotgun_dna.
- host run.host_tax_id · “9606”
- body site study.description · “fecal microbiome in healthy Saudi adults”
- life stage study.description · “healthy Saudi adults aged 19 to 69”
Evidence examples (one per field × route)
| sample | field | value | route | conf. | source | quote |
|---|---|---|---|---|---|---|
| SAMN53064657 | age_at_collection_days | 12043.2 | R1 | 0.85 | sample.attr.host_age | 33 |
| SAMN53064657 | collection_date | 2024-01-14 | R1 | 0.90 | biosample.attribute:collection_date | 2024-01-14 |
| SAMN53064657 | country | SA | R1 | 0.90 | sample.attr.geo_loc_name | Saudi Arabia: Riyadh |
| SAMN53064657 | health_condition | healthy_control | R3 | 0.70 | paper.fulltext.methods | 145 healthy Saudi adults were recruited for fecal microbiome analysis |
| SAMN53064657 | health_condition_detail | All 145 participants were healthy | R4 | 0.50 | paper.abstract | 145 healthy participants aged 19-69 years |
| SAMN53064657 | location_locality | Riyadh | R1 | 0.85 | biosample.attribute:geo_loc_name | Saudi Arabia: Riyadh |
| SAMN53064657 | sex | male | R1 | 0.90 | sample.attr.host_sex | male |
Sequencing
145 runs over 145 samples · mean 2.71 Gbp per run (median 2.16 Gbp, total 392.84 Gbp; 145 runs with a base count) · layout PAIRED 145 · platform ILLUMINA 145 · models NextSeq 2000 (145) · 100% of runs with a Sandpiper profile · first public 2025-11-11
Authors (12)
Almatrafi R first · Alasiri A · Almuneef G · Al-Hazzani AA · Alghoribi MF · Hakami M · Arafah AM · Alotibi RS · Alrabiah S · Alqurainy N · Ajina R · Aldriwesh MG last
Downloads
PRJNA1356643.csv.gz (5.2 KB) · parquet (76.8 KB) — all 145 samples, every field with route and confidence · PRJNA1356643_determinations.csv.gz (7.8 KB) — 1,015 evidence rows