Paired 16S V4, full-length 16S, shotgun comparison
Meta-analysis comparing short-read 16S V4, full-length 16S, and shotgun metagenomic profiling across 18 samples; no population, location, or health condition details are provided. machine-written summary
18 samples · 18 runs · Age unknown · 2025 · 13.40 Gbp / sample
Checked this project's papers and archive records yourself? Mark it human-verified.
what each source gave
| ✓ Sequence archive | 18 sample values (subject id) |
| – Abstract / description | no abstract or project description |
| – Full text | no open-access full text linked |
| – Supplementary tables | no supplementary tables available (or none open access) |
| – External resources | not covered by the external resources we ingest |
| – User contribution | nothing uploaded yet |
| – Human verified | not yet checked by a person · I checked this project — verify it |
longitudinal — not resolved
4 of 4 people sampled more than once (18 samples; median 4, up to 6 per person).
No Sandpiper profile for this project's samples yet.
| field | samples | coverage | R1 | R2 | R3 | R4 |
|---|---|---|---|---|---|---|
| age_category | 0 | 0% | ||||
| country | 0 | 0% | ||||
| health_condition | 0 | 0% | ||||
| subject_id | 18 | 100% | 18 |
Archive description
This study evaluates the concordance between amplicon and shotgun sequencing approaches for microbiome profiling. The dataset comprises a total of 54 samples derived from human fecal material and defined mock communities. To assess taxonomic resolution and technical biases, we generated matched data for each sample using three distinct sequencing strategies: (1) Short-read 16S rRNA gene sequencing targeting the V4 region (n=18); (2) Full-length 16S rRNA gene sequencing (n=18); and (3) Shotgun metagenomic sequencing (n=18). These data support the development of bioinformatic frameworks for harmonizing taxonomic assignments across variable-region amplicon, long-read amplicon, and shotgun metagenomic platforms.
How this project entered the registry
Classified as human and non-human (mixed) gut / stool shotgun DNA metagenome of age unknown by opus_adjudicated with confidence 0.70. Assay shotgun_dna.
- host study.description · “derived from human fecal material and defined mock communities”
- body site study.description · “human fecal material”
Evidence examples (one per field × route)
| sample | field | value | route | conf. | source | quote |
|---|---|---|---|---|---|---|
| SAMN53367923 | subject_id | NM1 | R1 | 0.85 | biosample.attribute:subject | subject: NM1 |
Sequencing
18 runs over 18 samples · mean 13.90 Gbp per run (median 13.40 Gbp, total 250.25 Gbp; 18 runs with a base count) · layout PAIRED 18 · platform ILLUMINA 18 · models Illumina NovaSeq 6000 (18) · 0% of runs with a Sandpiper profile · first public 2025-11-29 – 2025-11-30
Authors (0)
No author record.
Downloads
PRJNA1368707.csv.gz (1.2 KB) · parquet (68.7 KB) — all 18 samples, every field with route and confidence · PRJNA1368707_determinations.csv.gz (444 B) — 18 evidence rows