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PRJNA1372133 SRP669241

Beef consumption diet trial for ARG transmission risk

Metagenomic sequencing of fecal and beef samples Raw sequence reads

226 fecal and beef samples from a randomized, controlled, crossover dietary intervention trial investigating transmission of antimicrobial resistance genes to consumers through beef consumption. machine-written summary

162 samples · 226 runs · US · Age unknown · 2026 · 9.23 Gbp / sample

Sources
✓Sequence archive 806–Abstract / description–Full text–Supplementary tables–External resources–User contribution–Human verified

Checked this project's papers and archive records yourself? Mark it human-verified.

what each source gave
✓ Sequence archive806 sample values (collection date, country, location region, subject id, timepoint)
– Abstract / descriptionno abstract or project description
– Full textno open-access full text linked
– Supplementary tablesno supplementary tables available (or none open access)
– External resourcesnot covered by the external resources we ingest
– User contributionnothing uploaded yet · share metadata
– Human verifiednot yet checked by a person · I checked this project — verify it
Interventions

intervention — arms not resolved

Controlled / prescribed diet or feeding regimen design crossover · population healthy_control · Randomized crossover beef consumption dietary trial

“randomized, controlled, crossover dietary trial” — bioproject.description (study level; per-sample arms are in the intervention column below)

Samples at a glance
1 country
Age
unknown162
Country
US162
Body site
primary160
unknown2
Sequencing depth per sample · this project all projects
all projects, 0–0.03 Gbp: 25,080 samples (4 %)this project, 0–0.03 Gbp: 1 samplesall projects, 0.03–0.1 Gbp: 17,066 samples (3 %)all projects, 0.1–0.3 Gbp: 29,179 samples (5 %)all projects, 0.3–1 Gbp: 47,044 samples (8 %)all projects, 1–3 Gbp: 124,983 samples (22 %)all projects, 3–10 Gbp: 253,728 samples (44 %)this project, 3–10 Gbp: 138 samplesall projects, 10–30 Gbp: 72,533 samples (13 %)this project, 10–30 Gbp: 23 samplesall projects, 30–100 Gbp: 3,024 samples (1 %)all projects, 100–300 Gbp: 106 samples (0 %)all projects, ≥ 300 Gbp: 9 samples (0 %)project median 8.37 Gbp per sample0130Gbp
Sampling design and relatedness

longitudinal

26 of 26 people sampled more than once (160 samples; median 7, up to 7 per person) · follow-up median 3 mo (max 4 mo) · median interval between samples 14 d (time from collection dates). Schedule stated by the source: “Repeated fecal sampling across 7 timepoints (A-G) per subject in a crossover dietary intervention trial.”.

101101: day 0101: day 14101: day 21101: day 44102102: day 0102: day 15102: day 21102: day 49102: day 63102: day 71102: day 103103103: day 0103: day 14103: day 22103: day 52103: day 65103: day 70103: day 127104104: day 0104: day 12104: day 20104: day 49104: day 61104: day 69104: day 98105105: day 0105: day 13105: day 20105: day 49105: day 63105: day 69105: day 98106106: day 0106: day 4106: day 12106: day 40106: day 42106: day 60106: day 90108108: day 0108: day 30108: day 38108: day 63108: day 79108: day 86108: day 113110110: day 0110: day 20110: day 26110: day 56110: day 101110: day 107110: day 133111111: day 0111: day 16111: day 24111: day 51111: day 65111: day 71111: day 99113113: day 0113: day 29113: day 37113: day 101114114: day 0114: day 15114: day 21114: day 49114: day 63114: day 70114: day 102115115: day 0115: day 28115: day 34115: day 81117117: day 0117: day 13117: day 19118118: day 0118: day 11118: day 37120120: day 0120: day 14120: day 20120: day 52120: day 62120: day 71120: day 98121121: day 0121: day 14121: day 21121: day 50121: day 63121: day 95122122: day 0122: day 28122: day 35122: day 63122: day 77122: day 84122: day 123123123: day 0123: day 12123: day 19123: day 48123: day 62123: day 69123: day 99124124: day 0124: day 15124: day 23124: day 35124: day 64124: day 71124: day 99126126: day 0126: day 14126: day 25126: day 49126: day 70126: day 73126: day 97128128: day 0128: day 9128: day 17128: day 45128: day 59128: day 65128: day 97129129: day 0129: day 36129: day 41129: day 72129: day 83129: day 90129: day 125130130: day 0130: day 27130: day 31130: day 33130: day 77130: day 83130: day 110132132: day 0132: day 16132: day 21132: day 50132: day 64132: day 70132: day 98133133: day 0133: day 14133: day 21133: day 49133: day 64133: day 70133: day 98135135: day 0135: day 15135: day 210 d33 d2 mo3 mo4 mo

Each row is one person (26 shown); dots are samples at days since that person's first sample.

How longitudinal and relatedness information is derived

Community composition
Bacteroides 8.3%Faecalibacterium 6.2%Phocaeicola 6.0%Prevotella 4.6%Alistipes 4.5%Blautia_A 4.3%Roseburia 3.3%Bifidobacterium 3.2%Gemmiger 2.0%Vescimonas 1.6%CAG-103 1.0%Ruminococcoides 0.9%not assigned to a genus 39.9%other genera 14.4%

Genera, mean relative abundance over 158 profiled gut samples of this project (Sandpiper SingleM profiles, GTDB R232; low-depth and non-metagenome profiles left out).

Field coverage
fieldsamplescoverageR1R2R3R4
age_category0 0%
country162 100%162
health_condition0 0%
subject_id160 99%160
collection_date162 100%162
detailed_location162 100%
location_region162 100%162
timepoint_label160 99%160
Details
Archive description

Metagenomic sequencing samples from a randomized, controlled, crossover dietary trial to investigate the risk of transmitting antimicrobial resistance genes (ARG) to consumers through beef consumption.

How this project entered the registry

Classified as human and non-human (mixed) gut / stool / other human site shotgun DNA metagenome of age unknown by sonnet_x2 with confidence 0.50. Assay shotgun_dna.

  • host study.title · “Metagenomic sequencing of fecal and beef samples”
  • body site study.title · “fecal and beef samples”
Evidence examples (one per field × route)
samplefieldvaluerouteconf.sourcequote
SAMN54950209collection_date2021-03-08R10.90biosample.attribute:collection_date2021-03-08
SAMN54950209countryUSR10.90sample.attr.geo_loc_nameUSA: Indiana
SAMN54950209location_regionIndianaR10.85biosample.attribute:geo_loc_nameUSA: Indiana
SAMN54950209subject_id101R10.70sample.name_rule:source_material_id101A
SAMN54950209timepoint_labelAR10.70sample.name_rule:source_material_id101A
Sequencing

226 runs over 162 samples · mean 10.92 Gbp per run (median 9.23 Gbp, total 2,468.61 Gbp; 226 runs with a base count) · layout PAIRED 226 · platform ILLUMINA 226 · models Illumina NovaSeq X Plus (226) · 75% of runs with a Sandpiper profile · first public 2026-02-07

Authors (0)

No author record.

Help complete this project

2 of the 4 core fields are below 50% coverage: age_category, health_condition. Share metadata

Downloads

PRJNA1372133.csv.gz (5.5 KB) · parquet (76.1 KB) — all 162 samples, every field with route and confidence · PRJNA1372133_determinations.csv.gz (7.2 KB) — 806 evidence rows

Samples (162 · every field this project has values for · hover a cell for its source)
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