Gut microbiome in multiplex ASD families, Hong Kong
Shotgun metagenomic case-control study of 380 stool samples from children in multiplex, simplex, single-child ASD, and typically developing families in Hong Kong, examining family structure effects on gut microbiota. machine-written summary
380 samples · 380 runs · HK · Child · 2025 · 6.72 Gbp / sample
Checked this project's papers and archive records yourself? Mark it human-verified.
what each source gave
| ✓ Sequence archive | 1,982 sample values (collection date, country, latitude, location region, longitude, timepoint) |
| ○ Abstract / description | read; no usable statement |
| ✓ Full text | 380 sample values (antibiotic exposure) · PMC13062010 |
| ○ Supplementary tables | files read; no table could be matched to the samples |
| – External resources | not covered by the external resources we ingest |
| – User contribution | nothing uploaded yet · share metadata |
| – Human verified | not yet checked by a person · I checked this project — verify it |
families — not resolvedhouseholds — not resolvedsiblings — not resolved
429 children: sibling pairs from multiplex, simplex and TD families sharing a household; family ids not in archive.
Genera, mean relative abundance over 377 profiled gut samples of this project (Sandpiper SingleM profiles, GTDB R232; low-depth and non-metagenome profiles left out).
| field | samples | coverage | R1 | R2 | R3 | R4 |
|---|---|---|---|---|---|---|
| age_category | 380 | 100% | ||||
| country | 380 | 100% | 380 | |||
| health_condition | 0 | 0% | ||||
| subject_id | 0 | 0% | ||||
| antibiotic_exposure | 380 | 100% | 380 | |||
| collection_date | 380 | 100% | 380 | |||
| detailed_location | 380 | 100% | ||||
| latitude | 380 | 100% | 380 | |||
| location_region | 380 | 100% | 380 | |||
| longitude | 380 | 100% | 380 | |||
| timepoint_label | 82 | 22% | 82 |
- Gut microbiome composition and strain-sharing in multiplex autism spectrum disorder families. 2026 PMID 41748585 PMC13062010 own_data
Archive description
This study aimed to investigate gut microbiota changes in different family types with ASD. Here, we performed shotgun metagenomic sequencing in stool samples of 429 children.
How this project entered the registry
Classified as human gut / stool shotgun DNA metagenome of child by sonnet_x2 with confidence 0.65. Assay shotgun_dna.
- host run.host_tax_id · “9606”
- body site study.description · “stool samples of 429 children”
- life stage study.description · “stool samples of 429 children”
Evidence examples (one per field × route)
| sample | field | value | route | conf. | source | quote |
|---|---|---|---|---|---|---|
| SAMN54131709 | antibiotic_exposure | no | R3 | 0.70 | paper.fulltext.methods | use of probiotics, antidepressants, anti-epileptics, or antibiotics within the 1 month |
| SAMN54131709 | collection_date | 2021-06-30 | R1 | 0.90 | biosample.attribute:collection_date | 2021-06-30 |
| SAMN54131709 | country | HK | R1 | 0.90 | sample.attr.geo_loc_name | Hong Kong |
| SAMN54131709 | latitude | 22.3964 | R1 | 0.90 | biosample.attribute:lat_lon | 22.3964 N 114.1694 E |
| SAMN54131709 | location_region | Sha Tin | R1 | 0.60 | biosample.attribute:lat_lon | 22.3964 N 114.1694 E |
| SAMN54131709 | longitude | 114.1694 | R1 | 0.90 | biosample.attribute:lat_lon | 22.3964 N 114.1694 E |
| SAMN54131851 | timepoint_label | T1 | R1 | 0.70 | sample.name_rule:alias | alias: A0244-T1 |
Sequencing
380 runs over 380 samples · mean 7.15 Gbp per run (median 6.72 Gbp, total 2,716.40 Gbp; 380 runs with a base count) · layout PAIRED 380 · platform ILLUMINA 380 · models Illumina NovaSeq 6000 (380) · 99% of runs with a Sandpiper profile · first public 2025-12-17
Authors (16)
Lu W first · Wong OWH · Zhu J · Chen S · Tun HM · Wan Y · Xu Z · Cheung CP · Ching JYL · Cheong PK · Chan S · Wong S · Chan D · Chan FKL · Su Q · Ng SC last
Help complete this project
2 of the 4 core fields are below 50% coverage: health_condition, subject_id. Share metadata
Downloads
PRJNA1377943.csv.gz (10.0 KB) · parquet (84.4 KB) — all 380 samples, every field with route and confidence · PRJNA1377943_determinations.csv.gz (14.5 KB) — 2,362 evidence rows