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PRJNA1390426 SRP657498

Fecal metagenomes before/after cholecystectomy, longitudinal

Homo sapiens Raw sequence reads

51 fecal metagenome samples from patients undergoing cholecystectomy (gallbladder removal surgery), collected before and after the procedure in a longitudinal design. Country and age data are not reported. machine-written summary

51 samples · 51 runs · Age unknown · 2025 · 11.58 Gbp / sample

Sources
✓Sequence archive 153–Abstract / description–Full text–Supplementary tables–External resources–User contribution–Human verified

Checked this project's papers and archive records yourself? Mark it human-verified.

what each source gave
✓ Sequence archive153 sample values (health condition, health condition detail, intervention, subject id, timepoint)
– Abstract / descriptionno abstract or project description
– Full textno open-access full text linked
– Supplementary tablesno supplementary tables available (or none open access)
– External resourcesnot covered by the external resources we ingest
– User contributionnothing uploaded yet · share metadata
– Human verifiednot yet checked by a person · I checked this project — verify it
Interventions

intervention — arms not resolved

Surgical procedure studied before/after (bariatric surgery design single_arm_before_after · population other_disease · Cholecystectomy, stool sampled before and after surgery

“before and after cholecystectomy” — bioproject.description (study level; per-sample arms are in the intervention column below)

Samples at a glance
Age
unknown51
Condition
healthy_control17
Body site
primary51
Sequencing depth per sample · this project all projects
all projects, 0–0.03 Gbp: 25,080 samples (4 %)all projects, 0.03–0.1 Gbp: 17,066 samples (3 %)all projects, 0.1–0.3 Gbp: 29,179 samples (5 %)all projects, 0.3–1 Gbp: 47,044 samples (8 %)all projects, 1–3 Gbp: 124,983 samples (22 %)all projects, 3–10 Gbp: 253,728 samples (44 %)this project, 3–10 Gbp: 2 samplesall projects, 10–30 Gbp: 72,533 samples (13 %)this project, 10–30 Gbp: 49 samplesall projects, 30–100 Gbp: 3,024 samples (1 %)all projects, 100–300 Gbp: 106 samples (0 %)all projects, ≥ 300 Gbp: 9 samples (0 %)project median 11.58 Gbp per sample0130Gbp
Sampling design and relatedness

longitudinal — not resolved

17 of 34 people sampled more than once (34 samples; median 2, up to 2 per person). Schedule stated by the source: “before and after cholecystectomy (pre/post timepoints), 34 subjects with up to 2 samples each” (time origin: surgery).

How longitudinal and relatedness information is derived

Community composition
Bacteroides 9.3%Blautia_A 8.4%Phocaeicola 8.2%Mediterraneibacter 5.7%Roseburia 4.4%Bifidobacterium 3.9%Escherichia 3.3%Faecalibacterium 2.8%Megamonas 2.5%Streptococcus 2.1%Ruminococcoides 2.0%Enterococcus_B 2.0%not assigned to a genus 2.9%other genera 42.4%

Genera, mean relative abundance over 50 profiled gut samples of this project (Sandpiper SingleM profiles, GTDB R232; low-depth and non-metagenome profiles left out).

Field coverage
fieldsamplescoverageR1R2R3R4
age_category0 0%
country0 0%
health_condition17 33%17
subject_id51 100%51
intervention34 67%34
timepoint_label34 67%34
health_condition_detail17 33%17
Details
Archive description

Fecal metagenomic data of 51 patients before and after cholecystectomy

How this project entered the registry

Classified as human gut / stool shotgun DNA metagenome of age unknown by sonnet_x2 with confidence 0.72. Assay shotgun_dna.

  • host study.title · “Homo sapiens Raw sequence reads”
  • body site study.description · “Fecal metagenomic data of 51 patients”
Evidence examples (one per field × route)
samplefieldvaluerouteconf.sourcequote
SAMN54181574health_conditionhealthy_controlR10.70sample.name_rule:aliasHHCC1
SAMN54181574health_condition_detailHealthy / control (name token 'HHCC')R10.70sample.name_rule:aliasHHCC1
SAMN54181591interventionno_interventionR10.70sample.name_rule:aliasBLC1
SAMN54181574subject_idHHCC1R10.70sample.name_rule:aliasHHCC1
SAMN54181591timepoint_labelpreR10.70sample.name_rule:aliasBLC1
Sequencing

51 runs over 51 samples · mean 11.56 Gbp per run (median 11.58 Gbp, total 589.72 Gbp; 51 runs with a base count) · layout PAIRED 51 · platform ILLUMINA 51 · models Illumina NovaSeq 6000 (51) · 98% of runs with a Sandpiper profile · first public 2025-12-26 – 2025-12-27

Authors (0)

No author record.

Help complete this project

3 of the 4 core fields are below 50% coverage: age_category, country, health_condition. Share metadata

Downloads

PRJNA1390426.csv.gz (2.2 KB) · parquet (71.0 KB) — all 51 samples, every field with route and confidence · PRJNA1390426_determinations.csv.gz (1.8 KB) — 153 evidence rows

Samples (51 · every field this project has values for · hover a cell for its source)
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