Gut metagenome in osteoporosis and Treg regulation
118 gut metagenome samples studied in relation to postmenopausal osteoporosis, focusing on intestinal flora structure and regulatory T cells; no further sample, location, or design details are given. machine-written summary
118 samples · 118 runs · CN · Adult · 2026 · 5.02 Gbp / sample
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what each source gave
| ✓ Sequence archive | 236 sample values (collection date, country) |
| ✓ Abstract / description | 118 sample values (location site) |
| – Full text | no open-access full text linked |
| – Supplementary tables | no supplementary tables available (or none open access) |
| – External resources | not covered by the external resources we ingest |
| – User contribution | nothing uploaded yet · share metadata |
| – Human verified | not yet checked by a person · I checked this project — verify it |
No Sandpiper profile for this project's samples yet.
| field | samples | coverage | R1 | R2 | R3 | R4 |
|---|---|---|---|---|---|---|
| age_category | 0 | 0% | ||||
| country | 118 | 100% | 118 | |||
| health_condition | 0 | 0% | ||||
| subject_id | 0 | 0% | ||||
| collection_date | 118 | 100% | 118 | |||
| detailed_location | 118 | 100% | ||||
| location_site | 118 | 100% | 118 |
Archive description
Objective: To investigate the function and mechanisms of gut flora structural changes mediating regulatory T cells after postmenopausal osteoporosis.</p><p>Methods: From November 2022 to August 2023, 30 postmenopausal osteoporosis patients from the Spine Surgery Department and outpatient clinic of a local hospital, diagnosed by Dual - emission X - ray Absorptiometry (DXA) and related medical tests, were selected as the experimental group. Meanwhile, 30 postmenopausal non - osteoporosis patients from the physical examination department of the same local hospital, also diagnosed by DXA and related medical tests, were selected as the contrast group. Blood samples were collected for tests including beta - collagen special sequence assay (CROSSL), total type I collagen amino - terminal extended peptide assay (TP1NP), etc. Peripheral blood regulatory T cell CD4+CD25+ Foxp3+ cell (Treg) levels were detected by flow cytometry, and statistical analysis was done using SPSS 25.0. The correlation between chemical indicators and Treg cell levels was analyzed with Spearman's correlation coefficient. Fecal samples were collected and sequenced by high - throughput Metagenomics. We visualized gene, species and functional distributions, calculated diversities, performed clustering and PCA downscaling analyses. Wilcoxon, STAMP, LEfSe and KEGG pathway enrichment analyses were used to distinguish group differences, and Spearman correlation coefficient was used for the correlation between intestinal flora function and Treg cell levels. A P - value < 0.05 was considered significant.
How this project entered the registry
Classified as human gut / stool shotgun DNA metagenome of adult and elderly by opus_adjudicated with confidence 0.70. Assay shotgun_dna.
- host study.description · “30 postmenopausal osteoporosis patients”
- body site study.description · “Fecal samples were collected”
- life stage study.title · “postmenopausal osteoporosis”
Evidence examples (one per field × route)
| sample | field | value | route | conf. | source | quote |
|---|---|---|---|---|---|---|
| SAMN54324819 | collection_date | 2022-01-01 | R1 | 0.85 | biosample.attribute:collection_date | 2022-01-01 |
| SAMN54324819 | country | CN | R1 | 0.85 | biosample.attribute:geo_loc_name | China:Ordos |
| SAMN54324819 | location_site | Spine Surgery Department and outpatient clinic of a local hospital | R4 | 0.45 | ena.study_description | Spine Surgery Department and outpatient clinic of a local hospital |
Sequencing
118 runs over 118 samples · mean 4.98 Gbp per run (median 5.02 Gbp, total 587.91 Gbp; 118 runs with a base count) · layout PAIRED 118 · platform BGISEQ 118 · models MGISEQ-2000RS (118) · 0% of runs with a Sandpiper profile · first public 2026-01-05
Authors (0)
No author record.
Help complete this project
3 of the 4 core fields are below 50% coverage: age_category, health_condition, subject_id. Share metadata
Downloads
PRJNA1394613.csv.gz (3.4 KB) · parquet (73.8 KB) — all 118 samples, every field with route and confidence · PRJNA1394613_determinations.csv.gz (2.4 KB) — 354 evidence rows