Resistant starch RS2 vs RS4 gut microbiome crossover trial
341 stool samples from healthy adults in the USA, from a longitudinal crossover trial examining how dietary resistant starch types 2 and 4 versus a digestible control starch affect gut microbiome composition and function. machine-written summary
341 samples · 341 runs · US · Age unknown · 2026 · 9.96 Gbp / sample
Checked this project's papers and archive records yourself? Mark it human-verified.
what each source gave
| ✓ Sequence archive | 1,705 sample values (country, intervention, intervention detail, subject id, timepoint) |
| ✓ Abstract / description | 341 sample values (health condition detail) |
| ✓ Full text | 1,023 sample values (antibiotic exposure, collection date, health condition) · PMC13532264 |
| ○ Supplementary tables | files read; no table could be matched to the samples |
| – External resources | not covered by the external resources we ingest |
| – User contribution | nothing uploaded yet |
| – Human verified | not yet checked by a person · I checked this project — verify it |
intervention · arms resolved
Prebiotic / dietary fibre supplement design crossover · population healthy_control · RS2 and RS4 resistant starch supplementation vs digestible starch control, crossover
“dietary supplementation with two types of resistant starch (RS2 and RS4) and” — abstract:42496113 (study level; per-sample arms are in the intervention column below)
longitudinal — not resolved
58 of 68 people sampled more than once (331 samples; median 7, up to 7 per person). Schedule stated by the source: “Baseline, then Pre/End for each of three 10-day interventions (RS2, Ctl, RS4 in A/B crossover order) with 5-day washouts between interventions” (time origin: enrolment).
No Sandpiper profile for this project's samples yet.
| field | samples | coverage | R1 | R2 | R3 | R4 |
|---|---|---|---|---|---|---|
| age_category | 341 | 100% | ||||
| country | 341 | 100% | 341 | |||
| health_condition | 341 | 100% | 341 | |||
| subject_id | 341 | 100% | 341 | |||
| antibiotic_exposure | 341 | 100% | 341 | |||
| collection_date | 341 | 100% | 341 | |||
| health_condition_detail | 341 | 100% | 341 | |||
| intervention | 341 | 100% | 341 | |||
| intervention_detail | 341 | 100% | 341 | |||
| timepoint_label | 341 | 100% | 341 |
- Resistant starch types 2 and 4 induce distinct and reversible changes in the human gut microbiome. 2026 PMID 42496113 PMC13532264 own_data
Archive description
Longitudinal crossover shotgun metagenomics study to investigate the impact of dietary supplementation with two types of Resistant Starches (RS) on the human gut microbiome composition and functional potential. The stool samples used to generate the shotgun metagenomics sequencing data are also used in PRJNA944997.
How this project entered the registry
Classified as human gut / stool shotgun DNA metagenome of age unknown by sonnet_x2 with confidence 0.65. Assay shotgun_dna.
- host run.host_tax_id · “9606”
- body site run.scientific_name · “human gut metagenome”
Evidence examples (one per field × route)
| sample | field | value | route | conf. | source | quote |
|---|---|---|---|---|---|---|
| SAMN54601861 | antibiotic_exposure | no | R3 | 0.70 | paper.fulltext.methods | use of antibiotics 6 months before the start |
| SAMN54601861 | collection_date | 2020-10/2020-12 | R3 | 0.70 | paper.fulltext.methods | crossover dietary intervention conducted between October and December 2020 |
| SAMN54601861 | country | US | R1 | 0.90 | sample.attr.geo_loc_name | USA |
| SAMN54601861 | health_condition | healthy_control | R3 | 0.70 | paper.fulltext.methods | 68 healthy adults enrolled in a 7-week |
| SAMN54601861 | health_condition_detail | Healthy subjects enrolled in randomized clinical trial of dietary resistant starch supplementation | R4 | 0.50 | paper.abstract | randomized clinical trial |
| SAMN54601861 | intervention | no_intervention | R1 | 0.90 | biosample.attribute:timepoint_type | timepoint_type: Baseline |
| SAMN54601861 | intervention_detail | pre-crossover baseline, no intervention yet | R1 | 0.90 | biosample.attribute:timepoint_type | timepoint_type: Baseline |
| SAMN54601861 | subject_id | 320 | R1 | 0.85 | sample.attr.participant_id | 320 |
| SAMN54601861 | timepoint_label | Baseline | R1 | 0.85 | sample.attr.timepoint_type | Baseline |
Sequencing
341 runs over 341 samples · mean 9.90 Gbp per run (median 9.96 Gbp, total 3,374.54 Gbp; 341 runs with a base count) · layout PAIRED 341 · platform ILLUMINA 341 · models Illumina NovaSeq 6000 (341) · 0% of runs with a Sandpiper profile · first public 2026-07-16
Authors (9)
Piperni E first · Blanco-Míguez A · Mengoni C · Piccinno G · Punčochář M · Ren J · Segata N · Asnicar F · Poole AC last
Downloads
PRJNA1402699.csv.gz (9.7 KB) · parquet (82.8 KB) — all 341 samples, every field with route and confidence · PRJNA1402699_determinations.csv.gz (16.6 KB) — 3,069 evidence rows