Jejunal microbiome in obese patients pre bypass surgery
55 jejunal mucosa samples from severely obese patients undergoing Roux-en-Y bypass surgery, profiled by shotgun metagenomics in a cross-sectional design to assess jejunal microbiome composition and clinical correlations. machine-written summary
55 samples · 55 runs · HU · Age unknown · 2026 · 2.82 Gbp / sample
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what each source gave
| ✓ Sequence archive | 165 sample values (collection date, country, location locality) |
| ✓ Abstract / description | 110 sample values (health condition, health condition detail) |
| – Full text | no open-access full text linked |
| – Supplementary tables | no supplementary tables available (or none open access) |
| – External resources | not covered by the external resources we ingest |
| – User contribution | nothing uploaded yet · share metadata |
| – Human verified | not yet checked by a person · I checked this project — verify it |
intervention — arms not resolved
Surgical procedure studied before/after (bariatric surgery design observational_with_procedure · population obesity · Roux-en-Y gastric bypass surgery; jejunal mucosa sampled from excised tissue
“undergoing Roux-en-Y bypass surgery” — bioproject.description (study level; per-sample arms are in the intervention column below)
No Sandpiper profile for this project's samples yet.
| field | samples | coverage | R1 | R2 | R3 | R4 |
|---|---|---|---|---|---|---|
| age_category | 0 | 0% | ||||
| country | 55 | 100% | 55 | |||
| health_condition | 55 | 100% | 55 | |||
| subject_id | 0 | 0% | ||||
| collection_date | 55 | 100% | 55 | |||
| detailed_location | 55 | 100% | ||||
| health_condition_detail | 55 | 100% | 55 | |||
| location_locality | 55 | 100% | 55 |
Archive description
Introduction: Intestinal dysbiosis is a risk factor of obesity and its sequelae. Dysbiosis in easy-to-reach parts of the intestines is well-studied, in contrast to the less accessible parts, as the jejunum to which Roux-en-Y bypass surgery offers accessibility. In this study we assessed the jejunal microbiome of severely obese patients undergoing Roux-en-Y bypass surgery. Methods: The taxonomical composition of the jejunal microbiome was assessed in the mucosa of the excised jejunal sections by shotgun metagenomics. Correlations were assessed between the clinical records and the taxa of the microbiome. Results: A surprisingly large number of genera were identified in the jejunum among which classical intestinal, opportunistic intestinal and transient (i.e. food-associated) genera were detected. Based on the genera of the jejunal microbiome patients separated into three clusters (clusters 0-2) with differences in the clinical markers related to tissue damage/organ function. We identified multiple, although statistically weak associations between jejunal genera and the patients medical records, as metabolic (glycemic control and lipid metabolism markers), cardiovascular and tissue damage/organ function parameters. Among the correlations cluster 0 and 2 species appeared frequently, but cluster 1 species did not appear. Of the genera Limnospira, Limosilactobacillus, Phocaeicola, Alistipes, Streptomyces, Lactiplantibacillus, and Prevotella had multiple correlations with clinical markers. Conclusion: Hereby, we identified genera of the jejunal microbiome correlating with clinical markers of severe obesity.
How this project entered the registry
Classified as human gut / stool / blood / tissue / internal fluids shotgun DNA metagenome of age unknown by opus_adjudicated with confidence 0.70. Assay shotgun_dna.
- host study.title · “morbid obese patients”
- body site study.description · “in the mucosa of the excised jejunal sections”
Evidence examples (one per field × route)
| sample | field | value | route | conf. | source | quote |
|---|---|---|---|---|---|---|
| SAMN55359408 | collection_date | 2022-03-19 | R1 | 0.90 | biosample.attribute:collection_date | 2022-03-19 |
| SAMN55359408 | country | HU | R1 | 0.90 | sample.attr.geo_loc_name | Hungary:Veszprem |
| SAMN55359408 | health_condition | obesity | R4 | 0.50 | study.description | severely obese patients undergoing Roux-en-Y bypass |
| SAMN55359408 | health_condition_detail | morbid obesity / severe obesity | R4 | 0.50 | study.description | severely obese patients undergoing Roux-en-Y bypass |
| SAMN55359408 | location_locality | Veszprem | R1 | 0.85 | biosample.attribute:geo_loc_name | Hungary:Veszprem |
Sequencing
55 runs over 55 samples · mean 3.37 Gbp per run (median 2.82 Gbp, total 185.14 Gbp; 55 runs with a base count) · layout PAIRED 55 · platform ILLUMINA 55 · models Illumina MiSeq (55) · 0% of runs with a Sandpiper profile · first public 2026-09-02
Authors (0)
No author record.
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2 of the 4 core fields are below 50% coverage: age_category, subject_id. Share metadata
Downloads
PRJNA1423645.csv.gz (2.2 KB) · parquet (71.0 KB) — all 55 samples, every field with route and confidence · PRJNA1423645_determinations.csv.gz (1.9 KB) — 275 evidence rows