AG1 supplementation and gut microbiota in trained adults
79 samples from trained adults studying the effect of AG1 supplementation on nutritional adequacy and gut microbial composition. No further details on location or design are given. machine-written summary
79 samples · 79 runs · US · Adult · 2026 · 1.43 Gbp / sample
Checked this project's papers and archive records yourself? Mark it human-verified.
what each source gave
| ✓ Sequence archive | 237 sample values (country, subject id, timepoint) |
| ○ Abstract / description | read; no usable statement |
| ✓ Full text | 474 sample values (health condition, health condition detail, lifestyle, lifestyle detail, medication, medication detail) · PMC13077853 |
| ○ Supplementary tables | files read; no table could be matched to the samples |
| – External resources | not covered by the external resources we ingest |
| – User contribution | nothing uploaded yet |
| – Human verified | not yet checked by a person · I checked this project — verify it |
intervention — arms not resolved
Non-microbial dietary supplement (vitamins Synbiotic (probiotic + prebiotic) design randomized_controlled_trial · population healthy_control · AG1 (vitamin/mineral/prebiotic/probiotic supplement) vs placebo, crossover, 14 days each
“randomized, double-blind, placebo-controlled crossover study examined the effects” — abstract:41988471 (study level; per-sample arms are in the intervention column below)
longitudinal — not resolved
20 of 20 people sampled more than once (79 samples; median 4, up to 4 per person). Schedule stated by the source: “Stool collected at baseline and endpoint of each of two 14-day supplementation periods (AG1 and placebo, crossover with washout), yielding 4 timepoints (T1-T4) per subject.” (time origin: Start of first 14-day supplementation period).
No Sandpiper profile for this project's samples yet.
| field | samples | coverage | R1 | R2 | R3 | R4 |
|---|---|---|---|---|---|---|
| age_category | 79 | 100% | ||||
| country | 79 | 100% | 79 | |||
| health_condition | 79 | 100% | 79 | |||
| subject_id | 79 | 100% | 79 | |||
| health_condition_detail | 79 | 100% | 79 | |||
| lifestyle | 79 | 100% | 79 | |||
| lifestyle_detail | 79 | 100% | 79 | |||
| medication | 79 | 100% | 79 | |||
| medication_detail | 79 | 100% | 79 | |||
| timepoint_label | 79 | 100% | 79 |
- Effect of AG1® supplementation on nutritional adequacy and gut microbial composition in trained adults. 2026 PMID 41988471 PMC13077853 own_data
Archive description
Dietary supplements that combine vitamins, minerals, phytonutrients, prebiotics, and probiotics have gained popularity among health-oriented consumers seeking convenient ways to fill nutritional gaps and support gut health. Methods: This randomized, double-blind, placebo-controlled crossover study examined the effects of two weeks of the nutritional supplement (AG1) on gut microbial composition, nutritional adequacy, and tolerability. Twenty resistance-trained men (n=10 26.4 +/- 6.5 y) and women (n=10 26.9 +/- 5.3 y) supplemented daily with either AG1 or placebo (PL) for 14 days. Following a 2-week washout, participants crossed over to the other condition. Participants provided stool samples for gut microbial composition analysis, completed the Digestion-associated Quality of Life Questionnaire (DQLQ), and completed a 24-hour dietary intake assessment at the beginning and end of each 14-day supplementation period. Outcomes were analyzed using repeated-measures and multivariate statistical approaches for dietary intake, gut microbiota, metabolomics, and questionnaire data. Results: AG1 did not produce large, global shifts in microbial alpha or beta diversity, supplementation was associated with selective enrichment of key bacterial taxa commonly linked to gut health, including Lactiplantibacillus plantarum, Lacticaseibacillus rhamnosus, and Bifidobacterium animalis. AG1 supplementation significantly improved nutritional adequacy by increasing the total number of micronutrient Estimated Average Requirements (EARs) met compared to placebo (2.8 p = 0.0011), with no significant differences in digestive quality of life between groups (p = 0.777). Vitamins A, C, and E were the most common nutrient gaps filled by AG1 supplementation. Conclusions: Two weeks of AG1 supplementation improved micronutrient adequacy in healthy resistance-trained adults by reducing nutrient gaps. Supplementation also selectively enriched key beneficial gut microbial taxa and putative microbial functional without inducing major disruptions to overall community structure. Importantly, AG1 was well tolerated and did not negatively impact digestion-associated quality of life.
How this project entered the registry
Classified as human gut / stool shotgun DNA metagenome of adult by sonnet_x2 with confidence 0.75. Assay shotgun_dna.
- host study.description · “Twenty resistance-trained men”
- body site study.description · “Participants provided stool samples”
- life stage study.description · “26.4 +/- 6.5 y”
Evidence examples (one per field × route)
| sample | field | value | route | conf. | source | quote |
|---|---|---|---|---|---|---|
| SAMN56641128 | country | US | R1 | 0.90 | sample.attr.geo_loc_name | USA |
| SAMN56641128 | health_condition | healthy_control | R3 | 0.70 | paper.body.text | be free of any chronic illness, be free of any medication use |
| SAMN56641128 | health_condition_detail | free of any chronic illness, free of any medication use | R3 | 0.70 | paper.body.text | be free of any chronic illness, be free of any medication use |
| SAMN56641128 | lifestyle | athlete | R3 | 0.70 | paper.body.text | >1 year of resistance training experience (minimum of 2 days per week of resistance training) |
| SAMN56641128 | lifestyle_detail | resistance-trained men and women with >1 year resistance training experience | R3 | 0.70 | paper.body.text | >1 year of resistance training experience (minimum of 2 days per week of resistance training) |
| SAMN56641128 | medication | none_reported | R3 | 0.70 | paper.body.text | be free of any medication use |
| SAMN56641128 | medication_detail | free of any medication use (inclusion criterion) | R3 | 0.70 | paper.body.text | be free of any medication use |
| SAMN56641128 | subject_id | HF01 | R1 | 0.70 | sample.name_rule:library_names | HF01_T1A0 |
| SAMN56641128 | timepoint_label | T1 | R1 | 0.70 | sample.name_rule:library_names | HF01_T1A0 |
Sequencing
79 runs over 79 samples · mean 1.43 Gbp per run (median 1.43 Gbp, total 112.88 Gbp; 79 runs with a base count) · layout PAIRED 79 · platform ELEMENT 79 · models Element AVITI (79) · 0% of runs with a Sandpiper profile · first public 2026-04-02
Authors (0)
No author record.
Downloads
PRJNA1441102.csv.gz (2.9 KB) · parquet (73.6 KB) — all 79 samples, every field with route and confidence · PRJNA1441102_determinations.csv.gz (4.7 KB) — 711 evidence rows