Gut metagenomics in chronic constipation, prune trial
1248 gut metagenome samples from treatment-naive adults with chronic constipation and healthy controls, studying microbial functional dysregulation and its reshaping by a randomized, double-blind prune (dietary supplement) intervention. machine-written summary
1,248 samples · 1,248 runs · Adult · 2026 · 1.44 Gbp / sample
Checked this project's papers and archive records yourself? Mark it human-verified.
what each source gave
| ✓ Sequence archive | 1,248 sample values (subject id) |
| ✓ Abstract / description | 1,248 sample values (health condition detail) |
| – Full text | no open-access full text linked |
| – Supplementary tables | no supplementary tables available (or none open access) |
| – External resources | not covered by the external resources we ingest |
| – User contribution | nothing uploaded yet · share metadata |
| – Human verified | not yet checked by a person · I checked this project — verify it |
intervention — arms not resolved
Non-microbial dietary supplement (vitamins design randomized_controlled_trial · population other_disease · Prune intake vs placebo in chronic constipation
“randomized, double-blind prune intervention” — bioproject.description (study level; per-sample arms are in the intervention column below)
No Sandpiper profile for this project's samples yet.
| field | samples | coverage | R1 | R2 | R3 | R4 |
|---|---|---|---|---|---|---|
| age_category | 1,248 | 100% | ||||
| country | 0 | 0% | ||||
| health_condition | 0 | 0% | ||||
| subject_id | 1,248 | 100% | 1,248 | |||
| health_condition_detail | 1,248 | 100% | 1,248 |
Archive description
We conducted large-scale shotgun metagenomic sequencing of treatment-naive adults with chronic constipation and healthy controls, all free of comorbidities and medication use. Chronic constipation was associated with suppression of microbial carbohydrate metabolism, including pectin degradation, central carbon metabolism, and short-chain fatty acid production, together with altered bifid shunt-mediated carbon flux, GABA shunt, and hydrogen consumption, including methanogenesis. Additional perturbations in amino acid metabolism, bile acid metabolism, and microbial structural features indicated system-level metabolic dysfunction of the gut microbiome. We further conducted a randomized, double-blind prune intervention in the same cohort. In contrast to placebo, prune intake improved constipation symptoms and selectively restored the sequential processes from carbohydrate uptake to fermentation, reduced methanogenesis, and shifted constipation-associated microbial taxa toward a healthy reference state. This study reframes chronic constipation as a disorder driven by dysregulated host-microbial metabolic crosstalk that can be partially reshaped by natural food-based intervention.
How this project entered the registry
Classified as human gut / stool / blood / tissue / internal fluids shotgun DNA metagenome of adult by deterministic_prior with confidence 0.90. Assay shotgun_dna.
- host external_curation.infant_catalog · “infant triage reason_code age_adult_only”
- body site study.title · “System-level gut microbial functional dysregulation”
- body site study.description · “dysfunction of the gut microbiome. We further”
- body site study.description · “amino acid metabolism, bile acid metabolism, and”
- life stage external_curation.infant_catalog · “infant triage reason_code age_adult_only”
- life stage study.description · “sequencing of treatment-naive adults with chronic constipation”
Evidence examples (one per field × route)
| sample | field | value | route | conf. | source | quote |
|---|---|---|---|---|---|---|
| SAMN56793798 | health_condition_detail | mixed_case_control: chronic constipation patients vs healthy controls | R4 | 0.50 | study.description | chronic constipation and healthy controls |
| SAMN56793798 | subject_id | JP4D_5805 | R1 | 0.85 | sample.attr.host_subject_id | JP4D_5805 |
Sequencing
1,248 runs over 1,248 samples · mean 1.59 Gbp per run (median 1.44 Gbp, total 1,988.00 Gbp; 1,248 runs with a base count) · layout PAIRED 1,248 · platform ILLUMINA 1,248 · models Illumina HiSeq X (727), Illumina NovaSeq X (521) · 0% of runs with a Sandpiper profile · first public 2026-04-16
Authors (0)
No author record.
Help complete this project
2 of the 4 core fields are below 50% coverage: country, health_condition. Share metadata
Downloads
PRJNA1445561.csv.gz (29.8 KB) · parquet (120.4 KB) — all 1,248 samples, every field with route and confidence · PRJNA1445561_determinations.csv.gz (18.0 KB) — 2,496 evidence rows