PFOA effects on gut microbiome, ex vivo, healthy adults
54 fecal samples from healthy adults (25-70) were incubated ex vivo in the SIFR® simulator with or without PFOA (10 mg/L) for 48 hours, with community composition assessed by shotgun metagenomic sequencing. machine-written summary
54 samples · 54 runs · BE · Adult · 2026 · 0.55 Gbp / sample
Checked this project's papers and archive records yourself? Mark it human-verified.
what each source gave
| ✓ Sequence archive | 486 sample values (collection date, country, health condition, health condition detail, latitude, location region, longitude, subject id) |
| ○ Abstract / description | read; no usable statement |
| ○ Full text | read; no usable statement or table · PMC13297463 |
| ○ Supplementary tables | files read; no table could be matched to the samples |
| – External resources | not covered by the external resources we ingest |
| – User contribution | nothing uploaded yet |
| – Human verified | not yet checked by a person · I checked this project — verify it |
longitudinal
18 of 18 people sampled more than once (54 samples; median 3, up to 3 per person) · follow-up median 2 d (max 2 d) · median interval between samples 2 d (time from timepoint labels).
Each row is one person (17 shown of 18); dots are samples at days since that person's first sample.
No Sandpiper profile for this project's samples yet.
| field | samples | coverage | R1 | R2 | R3 | R4 |
|---|---|---|---|---|---|---|
| age_category | 54 | 100% | ||||
| country | 54 | 100% | 54 | |||
| health_condition | 54 | 100% | 54 | |||
| subject_id | 54 | 100% | 54 | |||
| collection_date | 54 | 100% | 54 | |||
| detailed_location | 54 | 100% | ||||
| health_condition_detail | 54 | 100% | 54 | |||
| latitude | 54 | 100% | 54 | |||
| location_region | 54 | 100% | 54 | |||
| longitude | 54 | 100% | 54 | |||
| timepoint_label | 54 | 100% | 54 |
- Perfluorooctanoic Acid (PFOA) Alters the Structure of the Gut Microbial Community and Colonoid Transcription 2026 PMID 42353547 own_data
- Perfluorooctanoic Acid (PFOA) Alters the Structure of the Gut Microbial Community and Colonoid Transcription 2026 PMC13297463 unsure
Archive description
This study uses ex vivo incubation of human fecal samples to study the effects of commonly occurring food additives or contaminants on the gut microbiome. Samples were incubated for 48 hours and include untreated starting inoculum, untreated controls and treated samples. Community composition is assessed using shotgun metagenomic sequencing.
How this project entered the registry
Classified as human gut / stool shotgun DNA metagenome of adult by sonnet_x2 with confidence 0.72. Assay shotgun_dna.
- host study.title · “human gut microbiome tested ex vivo”
- body site study.description · “ex vivo incubation of human fecal samples”
- life stage sample.attr.host_age_group · “Healthy_adult_25-35 (n=54/54)”
Evidence examples (one per field × route)
| sample | field | value | route | conf. | source | quote |
|---|---|---|---|---|---|---|
| SAMN57391023 | collection_date | 2023-10 | R1 | 0.90 | biosample.attribute:collection_date | 2023-10 |
| SAMN57391023 | country | BE | R1 | 0.90 | sample.attr.geo_loc_name | Belgium |
| SAMN57391023 | health_condition | healthy_control | R1 | 0.90 | biosample_attr | Healthy_adult_25-35 |
| SAMN57391023 | health_condition_detail | Healthy_adult_25-35 | R1 | 0.85 | biosample.attribute:host_age_group | Healthy_adult_25-35 |
| SAMN57391023 | latitude | 51.0500 | R1 | 0.90 | biosample.attribute:lat_lon | 51.05 N 3.73333 E |
| SAMN57391023 | location_region | Flanders | R1 | 0.60 | biosample.attribute:lat_lon | 51.05 N 3.73333 E |
| SAMN57391023 | longitude | 3.7333 | R1 | 0.90 | biosample.attribute:lat_lon | 51.05 N 3.73333 E |
| SAMN57391023 | subject_id | P0109_Donor_1 | R1 | 0.85 | sample.attr.subject_id | P0109_Donor_1 |
| SAMN57391023 | timepoint_label | 0h | R1 | 0.85 | biosample.attribute:incubation_timepoint | 0h |
Sequencing
54 runs over 54 samples · mean 0.58 Gbp per run (median 0.55 Gbp, total 31.19 Gbp; 54 runs with a base count) · layout PAIRED 54 · platform ILLUMINA 54 · models Illumina NovaSeq X Plus (54) · 0% of runs with a Sandpiper profile · first public 2026-05-20
Authors (12)
Liu L first · Narrowe AB · Firrman J · Mahalak KK · Chetty VJ · Lemons JMS · Baudot A · Van den Abbeele P last · Narrowe A · Mahalak K · Chetty V · Lemons J
Downloads
PRJNA1456031.csv.gz (2.2 KB) · parquet (72.0 KB) — all 54 samples, every field with route and confidence · PRJNA1456031_determinations.csv.gz (3.7 KB) — 486 evidence rows