Human Fecal Metagenome in Healthy Adults, Cross-Sectional
Whole-metagenome shotgun sequencing of 100 fecal samples from healthy adult donors, using a cross-sectional design to characterize gut bacterial composition and functional potential. machine-written summary
100 samples · 100 runs · CN · Adult · 2026 · 7.6 Mbp / sample
Checked this project's papers and archive records yourself? Mark it human-verified.
what each source gave
| ✓ Sequence archive | 300 sample values (collection date, country, location region) |
| ✓ Abstract / description | 200 sample values (health condition, health condition detail) |
| – Full text | no open-access full text linked |
| – Supplementary tables | no supplementary tables available (or none open access) |
| – External resources | not covered by the external resources we ingest |
| – User contribution | nothing uploaded yet |
| – Human verified | not yet checked by a person · I checked this project — verify it |
No Sandpiper profile for this project's samples yet.
| field | samples | coverage | R1 | R2 | R3 | R4 |
|---|---|---|---|---|---|---|
| age_category | 100 | 100% | ||||
| country | 100 | 100% | 100 | |||
| health_condition | 100 | 100% | 100 | |||
| subject_id | 0 | 0% | ||||
| collection_date | 100 | 100% | 100 | |||
| detailed_location | 100 | 100% | ||||
| health_condition_detail | 100 | 100% | 100 | |||
| location_region | 100 | 100% | 100 |
Archive description
The human gut microbiome plays a crucial role in host metabolism, immune function, and overall health. This project employed whole-metagenome shotgun sequencing to characterize the bacterial community composition and functional potential of human fecal samples. Total microbial community DNA was extracted from fecal specimens collected from 48 healthy adult donors. Sequencing was performed on the Illumina NovaSeq 6000 platform, generating paired-end reads of 150 bp. Raw sequence data are being submitted to the NCBI Sequence Read Archive (SRA) under this BioProject. This dataset will serve as a valuable resource for comparative studies of the gut microbiota, facilitating investigations into associations between gut microbial composition and host physiology, as well as for the recovery of metagenome-assembled genomes (MAGs) and functional gene analysis.
How this project entered the registry
Classified as human gut / stool shotgun DNA metagenome of adult by deterministic_prior with confidence 0.90. Assay shotgun_dna.
- host external_curation.infant_catalog · “infant triage reason_code age_adult_only”
- body site study.title · “Human Fecal Bacteria Metagenome”
- body site study.description · “The human gut microbiome plays a”
- life stage external_curation.infant_catalog · “infant triage reason_code age_adult_only”
- life stage study.description · “from 48 healthy adult donors. Sequencing was”
Evidence examples (one per field × route)
| sample | field | value | route | conf. | source | quote |
|---|---|---|---|---|---|---|
| SAMN58887345 | collection_date | 2022-12-14 | R1 | 0.90 | biosample.attribute:collection_date | 2022-12-14 |
| SAMN58887345 | country | CN | R1 | 0.90 | sample.attr.geo_loc_name | China: Shandong |
| SAMN58887345 | health_condition | healthy_control | R4 | 0.50 | study.description | 48 healthy adult donors |
| SAMN58887345 | health_condition_detail | healthy adults | R4 | 0.50 | study.description | 48 healthy adult donors |
| SAMN58887345 | location_region | Shandong | R1 | 0.85 | biosample.attribute:geo_loc_name | China: Shandong |
Sequencing
100 runs over 100 samples · mean 7.5 Mbp per run (median 7.6 Mbp, total 0.75 Gbp; 100 runs with a base count) · layout PAIRED 100 · platform ILLUMINA 100 · models Illumina HiSeq 2000 (100) · 0% of runs with a Sandpiper profile · first public 2026-08-22
Authors (0)
No author record.
Downloads
PRJNA1463012.csv.gz (2.2 KB) · parquet (72.1 KB) — all 100 samples, every field with route and confidence · PRJNA1463012_determinations.csv.gz (3.4 KB) — 500 evidence rows