DASH diet crossover trial in US adults with type 2 diabetes
438 gut microbiome samples from adults with type 2 diabetes in the USA, collected in a randomized, four-period crossover feeding trial comparing a DASH-style diet to a typical US diet. machine-written summary
438 samples · 438 runs · US · Adult · 2026 · 1.96 Gbp / sample
Checked this project's papers and archive records yourself? Mark it human-verified.
what each source gave
| ✓ Sequence archive | 2,628 sample values (country, latitude, location region, longitude, subject id, timepoint) |
| ✓ Abstract / description | 876 sample values (health condition, health condition detail) |
| – Full text | no open-access full text linked |
| – Supplementary tables | no supplementary tables available (or none open access) |
| – External resources | not covered by the external resources we ingest |
| – User contribution | nothing uploaded yet |
| – Human verified | not yet checked by a person · I checked this project — verify it |
intervention — arms not resolved
Controlled / prescribed diet or feeding regimen design crossover · population type2_diabetes · DASH-style diet vs typical US diet, 5-week feeding periods
“randomized, four-period crossover feeding trial, 97 participants consumed” — bioproject.description (study level; per-sample arms are in the intervention column below)
longitudinal — not resolved
94 of 94 people sampled more than once (438 samples; median 5, up to 5 per person). Schedule stated by the source: “Baseline sample plus samples after each of four 5-week feeding periods (crossover design)” (time origin: intervention start).
No Sandpiper profile for this project's samples yet.
| field | samples | coverage | R1 | R2 | R3 | R4 |
|---|---|---|---|---|---|---|
| age_category | 438 | 100% | ||||
| country | 438 | 100% | 438 | |||
| health_condition | 438 | 100% | 438 | |||
| subject_id | 438 | 100% | 438 | |||
| detailed_location | 438 | 100% | ||||
| health_condition_detail | 438 | 100% | 438 | |||
| latitude | 438 | 100% | 438 | |||
| location_region | 438 | 100% | 438 | |||
| longitude | 438 | 100% | 438 | |||
| timepoint_label | 438 | 100% | 438 |
Archive description
The Dietary Approaches to Stop Hypertension for Adults with Diabetes (DASH4D) study evaluated the effects of a DASH-style diet on the gut microbiome of adults with type 2 diabetes. In this randomized, four-period crossover feeding trial, 97 participants consumed 5-week periods of either a DASH-style diet or a typical US diet and provided samples at baseline and after each feeding period. Compared with the typical US diet, the DASH4D diet significantly increased gut microbiome diversity, altered overall microbial community composition, and changed the abundance of 13 microbial species. The diet enriched Faecalibacterium prausnitzii, an important butyrate-producing bacterium, increased a butyrate-producer score, and enhanced expression of the gene encoding butyryl-CoA:acetate CoA-transferase, the terminal enzyme in butyrate synthesis. The DASH4D diet also increased circulating acetate concentrations. These findings indicate that a DASH-style diet promotes beneficial gut microbiome characteristics and short-chain fatty acid production in adults with type 2 diabetes.
How this project entered the registry
Classified as human gut / stool shotgun DNA metagenome of adult by sonnet_x2 with confidence 0.72. Assay shotgun_dna.
- host run.host_tax_id · “9606”
- body site study.description · “gut microbiome of adults with type 2 diabetes”
- life stage study.description · “adults with type 2 diabetes”
Evidence examples (one per field × route)
| sample | field | value | route | conf. | source | quote |
|---|---|---|---|---|---|---|
| SAMN62088319 | country | US | R1 | 0.90 | sample.attr.geo_loc_name | USA |
| SAMN62088319 | health_condition | type2_diabetes | R4 | 0.50 | study.description | adults with type 2 diabetes |
| SAMN62088319 | health_condition_detail | type 2 diabetes | R4 | 0.50 | study.description | adults with type 2 diabetes |
| SAMN62088319 | latitude | 39.9473 | R1 | 0.90 | biosample.attribute:lat_lon | 39.9473 N 75.1958 W |
| SAMN62088319 | location_region | Pennsylvania | R1 | 0.60 | biosample.attribute:lat_lon | 39.9473 N 75.1958 W |
| SAMN62088319 | longitude | -75.1958 | R1 | 0.90 | biosample.attribute:lat_lon | 39.9473 N 75.1958 W |
| SAMN62088319 | subject_id | D0011MCAU | R1 | 0.70 | sample.name_rule:alias | D0011MCAU.FP2.Micro.Fe1 |
| SAMN62088319 | timepoint_label | feeding period 2 | R1 | 0.70 | sample.name_rule:alias | D0011MCAU.FP2.Micro.Fe1 |
Sequencing
438 runs over 438 samples · mean 1.99 Gbp per run (median 1.96 Gbp, total 870.42 Gbp; 438 runs with a base count) · layout PAIRED 438 · platform ILLUMINA 438 · models NextSeq 2000 (438) · 0% of runs with a Sandpiper profile · first public 2026-08-02
Authors (0)
No author record.
Downloads
PRJNA1505569.csv.gz (11.8 KB) · parquet (85.5 KB) — all 438 samples, every field with route and confidence · PRJNA1505569_determinations.csv.gz (22.5 KB) — 3,504 evidence rows