Metagenomics in major depressive disorder
Shotgun metagenomic sequencing of 23 fecal samples from adults with moderate to severe major depressive disorder (before and after treatment) and healthy controls, using a case-control design with taxonomic and functional (GO/KEGG) profiling. machine-written summary
23 samples · 23 runs · CN · Adult · 2026 · 5.16 Gbp / sample
Checked this project's papers and archive records yourself? Mark it human-verified.
what each source gave
| ✓ Sequence archive | 138 sample values (age, collection date, country, sex, subject id, timepoint) |
| – Abstract / description | no abstract or project description |
| – Full text | no open-access full text linked |
| – Supplementary tables | no supplementary tables available (or none open access) |
| – External resources | not covered by the external resources we ingest |
| – User contribution | nothing uploaded yet |
| – Human verified | not yet checked by a person · I checked this project — verify it |
longitudinal
6 of 6 people sampled more than once (23 samples; median 4, up to 5 per person) · follow-up median 43 d (max 2 mo) · median interval between samples 14 d (time from collection dates). Schedule stated by the source: “before and after treatment (multiple timepoints per subject, T1-T5)” (time origin: intervention_start).
Each row is one person (6 shown); dots are samples at days since that person's first sample.
No Sandpiper profile for this project's samples yet.
| field | samples | coverage | R1 | R2 | R3 | R4 |
|---|---|---|---|---|---|---|
| age_category | 23 | 100% | ||||
| country | 23 | 100% | 23 | |||
| health_condition | 0 | 0% | ||||
| subject_id | 23 | 100% | 23 | |||
| age_at_collection_days | 23 | 100% | 23 | |||
| collection_date | 23 | 100% | 23 | |||
| sex | 23 | 100% | 23 | |||
| timepoint_label | 23 | 100% | 23 |
Archive description
Fecal samples were collected from patients with moderate to severe major depressive disorder before and after treatment, as well as from a healthy control group. The fecal microbiota was analyzed using the NovaSeq 6000 platform. The obtained sequencing data were preprocessed and then subjected to de novo assembly. Non redundant Unigene sets were generated through gene prediction. Species level taxonomic information was obtained by aligning the Unigenes against the NR meta database, followed by alpha and beta diversity analyses. The Unigenes were also aligned against functional databases such as GO and KEGG to obtain annotation information. Finally, abundance statistics and differential comparisons of the fecal microbiota were performed at the taxonomic, functional annotation, and Unigene levels, and GO and KEGG enrichment analyses were conducted for the differentially expressed Unigenes.
How this project entered the registry
Classified as human gut / stool shotgun DNA metagenome of adult by deterministic_prior with confidence 0.90. Assay shotgun_dna.
- host external_curation.infant_catalog · “infant triage reason_code age_adult_only”
- body site study.title · “sequencing analysis of fecal microbiota patients with”
- body site study.description · “Fecal samples were collected”
- life stage external_curation.infant_catalog · “infant triage reason_code age_adult_only”
Evidence examples (one per field × route)
| sample | field | value | route | conf. | source | quote |
|---|---|---|---|---|---|---|
| SAMN62523262 | age_at_collection_days | 10601.0 | R1 | 0.85 | sample.attr.host_age | 29 |
| SAMN62523262 | collection_date | 2023-11-17 | R1 | 0.90 | biosample.attribute:collection_date | 2023-11-17 |
| SAMN62523262 | country | CN | R1 | 0.90 | sample.attr.geo_loc_name | China |
| SAMN62523262 | sex | male | R1 | 0.90 | sample.attr.host_sex | male |
| SAMN62523262 | subject_id | Subject_CX | R1 | 0.85 | sample.attr.host_subject_id | Subject_CX |
| SAMN62523262 | timepoint_label | T1 | R1 | 0.70 | sample.name_rule:library_names | CX1_MG |
Sequencing
23 runs over 23 samples · mean 5.18 Gbp per run (median 5.16 Gbp, total 119.24 Gbp; 23 runs with a base count) · layout PAIRED 23 · platform ILLUMINA 23 · models Illumina NovaSeq 6000 (23) · 0% of runs with a Sandpiper profile · first public 2026-08-23
Authors (0)
No author record.
Downloads
PRJNA1514834.csv.gz (1.6 KB) · parquet (70.5 KB) — all 23 samples, every field with route and confidence · PRJNA1514834_determinations.csv.gz (1.5 KB) — 138 evidence rows