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PRJNA1516599 SRP729284

Pea vs egg protein diet & gut microbiota, healthy adults, US

Dietary protein source impacts the gut microbiota in healthy humans

34 fecal metagenome samples from healthy adults in Stony Brook, NY, USA, collected in a controlled feeding intervention comparing pea protein and egg white protein diets over 10-day phases. machine-written summary

34 samples · 34 runs · US · Adult · 2026 · 6.69 Gbp / sample

Sources
✓Sequence archive 510○Abstract / description–Full text–Supplementary tables–External resources–User contribution–Human verified

Checked this project's papers and archive records yourself? Mark it human-verified.

what each source gave
✓ Sequence archive510 sample values (age, collection date, country, diet, diet detail, health condition, health condition detail, intervention)
○ Abstract / descriptionread; no usable statement
– Full textno open-access full text linked
– Supplementary tablesno supplementary tables available (or none open access)
– External resourcesnot covered by the external resources we ingest
– User contributionnothing uploaded yet
– Human verifiednot yet checked by a person · I checked this project — verify it
Interventions

intervention — arms not resolved

Controlled / prescribed diet or feeding regimen design crossover · population healthy_control · Pea protein vs egg white protein diet, 10 days

“pea protein or egg white protein-based diet” — bioproject.description (study level; per-sample arms are in the intervention column below)

Samples at a glance
1 sampling locations · 1 country
Age
adult34
Condition
healthy_control34
Sex
male22
female12
Country
US34
Body site
primary34
Sequencing depth per sample · this project all projects
all projects, 0–0.03 Gbp: 25,080 samples (4 %)all projects, 0.03–0.1 Gbp: 17,066 samples (3 %)all projects, 0.1–0.3 Gbp: 29,179 samples (5 %)all projects, 0.3–1 Gbp: 47,044 samples (8 %)all projects, 1–3 Gbp: 124,983 samples (22 %)all projects, 3–10 Gbp: 253,728 samples (44 %)this project, 3–10 Gbp: 34 samplesall projects, 10–30 Gbp: 72,533 samples (13 %)all projects, 30–100 Gbp: 3,024 samples (1 %)all projects, 100–300 Gbp: 106 samples (0 %)all projects, ≥ 300 Gbp: 9 samples (0 %)project median 6.69 Gbp per sample0130Gbp
Sampling design and relatedness

longitudinal

17 of 17 people sampled more than once (34 samples; median 2, up to 2 per person) · follow-up median 26 d (max 28 d) · median interval between samples 26 d (time from collection dates). Schedule stated by the source: “Fecal sample collected after each 10-day diet phase (pea protein, egg white protein) in crossover design” (time origin: intervention_start).

22: day 02: day 262323: day 023: day 262828: day 028: day 283030: day 030: day 263131: day 031: day 263939: day 039: day 264242: day 042: day 264747: day 047: day 265555: day 055: day 2666: day 06: day 286262: day 062: day 286565: day 065: day 286666: day 066: day 286767: day 067: day 2877: day 07: day 267171: day 071: day 287272: day 072: day 280 d7 d14 d21 d28 d

Each row is one person (17 shown); dots are samples at days since that person's first sample.

How longitudinal and relatedness information is derived

Community composition

No Sandpiper profile for this project's samples yet.

Field coverage
fieldsamplescoverageR1R2R3R4
age_category34 100%
country34 100%34
health_condition34 100%34
subject_id34 100%34
age_at_collection_days34 100%34
collection_date34 100%34
detailed_location34 100%
diet34 100%34
diet_detail34 100%34
health_condition_detail34 100%34
intervention34 100%34
intervention_detail34 100%34
latitude34 100%34
location_locality34 100%34
location_region34 100%34
longitude34 100%34
sex34 100%34
Papers (1)
  • Diet-derived peptides mediate the effects of dietary protein source on gastrointestinal health 2026 own_data
Details
Archive description

In a controlled feeding trial, healthy adults consumed either a pea protein or egg white protein-based diet for 10 days. Fecal metagenomic analysis was conducted after each diet phase to assess microbial populations and microbial gene abundance.

How this project entered the registry

Classified as human gut / stool shotgun DNA metagenome of adult by sonnet_x2 with confidence 0.75. Assay shotgun_dna.

  • host run.scientific_name · “human metagenome”
  • body site study.description · “Fecal metagenomic analysis was conducted”
  • life stage study.title · “impacts the gut microbiota in healthy humans”
  • life stage study.description · “healthy adults consumed either a pea protein”
Evidence examples (one per field × route)
samplefieldvaluerouteconf.sourcequote
SAMN62612585age_at_collection_days8211.0R10.85sample.attr.host_age22.5
SAMN62612585collection_date2023-03-02R10.90biosample.attribute:collection_date2023-03-02
SAMN62612585countryUSR10.90sample.attr.geo_loc_nameUSA: Stony Brook, NY
SAMN62612585diettherapeutic_or_study_dietR10.90biosample.attribute:host_diet70% Egg White Protein Formulated Diet
SAMN62612585diet_detail70% Egg White Protein Formulated DietR10.90biosample.attribute:host_diet70% Egg White Protein Formulated Diet
SAMN62612585health_conditionhealthy_controlR10.90biosample_attrHealthy
SAMN62612585health_condition_detailHealthyR10.90sample.attr.host_diseaseHealthy
SAMN62612585interventiondiet_interventionR10.90biosample.attribute:host_diethost_diet: 70% Egg White Protein Formulated Diet
SAMN62612585intervention_detailcontrolled 70% egg white protein dietR10.90biosample.attribute:host_diethost_diet: 70% Egg White Protein Formulated Diet
SAMN62612585latitude40.9000R10.90biosample.attribute:lat_lon40.90 N 73.13 W
SAMN62612585location_localityStony BrookR10.85biosample.attribute:geo_loc_nameUSA: Stony Brook, NY
SAMN62612585location_regionNew YorkR10.75biosample.attribute:geo_loc_nameUSA: Stony Brook, NY
SAMN62612585longitude-73.1300R10.90biosample.attribute:lat_lon40.90 N 73.13 W
SAMN62612585sexmaleR10.90sample.attr.host_sexmale
SAMN62612585subject_id2R10.85sample.attr.host_subject_id2
Sequencing

34 runs over 34 samples · mean 6.55 Gbp per run (median 6.69 Gbp, total 222.86 Gbp; 34 runs with a base count) · layout PAIRED 34 · platform ILLUMINA 34 · models Illumina HiSeq X (34) · 0% of runs with a Sandpiper profile · first public 2026-08-23

Authors (0)

No author record.

Downloads

PRJNA1516599.csv.gz (2.1 KB) · parquet (73.1 KB) — all 34 samples, every field with route and confidence · PRJNA1516599_determinations.csv.gz (4.1 KB) — 510 evidence rows

Samples (34 · every field this project has values for · hover a cell for its source)
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