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PRJNA245336 SRP049631

Gut/oral/skin microbiome, isolated Yanomami village

Uncontacted Yanomami resistome

Cross-sectional study of 22 fecal, oral, and skin microbiota samples from an isolated, previously uncontacted Yanomami Amerindian village in Venezuela, characterizing microbiome diversity and functional antibiotic resistance genes. machine-written summary

22 samples · 22 runs · VE · Age unknown · 2015 · 38.9 Mbp / sample

Sources
✓Sequence archive 132○Abstract / description○Full text○Supplementary tables–External resources–User contribution–Human verified

Checked this project's papers and archive records yourself? Mark it human-verified.

what each source gave
✓ Sequence archive132 sample values (age, antibiotic exposure, collection date, country, sex, subject id)
○ Abstract / descriptionread; no usable statement
○ Full textread; no usable statement or table · PMC4517851
○ Supplementary tablesfiles read; no table could be matched to the samples
– External resourcesnot covered by the external resources we ingest
– User contributionnothing uploaded yet
– Human verifiednot yet checked by a person · I checked this project — verify it
Samples at a glance
1 country
Age
adult14
adolescent4
child4
Sex
male14
female8
Country
VE22
Body site
excluded14
primary8
Sequencing depth per sample · this project all projects
all projects, 0–0.03 Gbp: 25,080 samples (4 %)this project, 0–0.03 Gbp: 10 samplesall projects, 0.03–0.1 Gbp: 17,066 samples (3 %)this project, 0.03–0.1 Gbp: 8 samplesall projects, 0.1–0.3 Gbp: 29,179 samples (5 %)this project, 0.1–0.3 Gbp: 3 samplesall projects, 0.3–1 Gbp: 47,044 samples (8 %)this project, 0.3–1 Gbp: 1 samplesall projects, 1–3 Gbp: 124,983 samples (22 %)all projects, 3–10 Gbp: 253,728 samples (44 %)all projects, 10–30 Gbp: 72,533 samples (13 %)all projects, 30–100 Gbp: 3,024 samples (1 %)all projects, 100–300 Gbp: 106 samples (0 %)all projects, ≥ 300 Gbp: 9 samples (0 %)project median 0.04 Gbp per sample0130Gbp
Sampling design and relatedness

community — not resolved

15 individuals from one isolated, previously uncontacted Yanomami village in Venezuela; no family/dyad structure stated..

How longitudinal and relatedness information is derived

Community composition
Prevotella 29.1%Cryptobacteroides 10.0%Phascolarctobacterium_A 2.2%not assigned to a genus 58.7%

Genera, mean relative abundance over 1 profiled gut samples of this project (Sandpiper SingleM profiles, GTDB R232; low-depth and non-metagenome profiles left out).

Field coverage
fieldsamplescoverageR1R2R3R4
age_category22 100%
country22 100%22
health_condition0 0%
subject_id22 100%22
age_at_collection_days22 100%22
antibiotic_exposure22 100%22
collection_date22 100%22
sex22 100%22
Papers (2)
  • The microbiome of uncontacted Amerindians. 2015 PMID 26229982 PMC4517851 own_data
  • High conservation of the dental plaque microbiome community across populations with differing subsistence strategies and levels of market integration 2022 unsure
Details
Archive description

Antibiotic resistance genes isolated from fecal and oral microbiota and E. coli isolates of previously uncontacted Yanomami Amerindians and Puerto Rican individuals

How this project entered the registry

Classified as human gut / stool / oral cavity shotgun DNA metagenome of adult and child and adolescent by sonnet_x2 with confidence 0.60. Assay shotgun_dna.

  • host run.host_tax_id · “9606”
  • body site study.description · “fecal and oral microbiota”
  • life stage sample.attr.age · “20 years (n=11/22)”
  • life stage sample.attr.age · “11 years (n=6/22)”
  • life stage sample.attr.age · “19 (n=5/22)”
Evidence examples (one per field × route)
samplefieldvaluerouteconf.sourcequote
SAMN02736856age_at_collection_days4018.0R10.90sample.attr.host_age11 years
SAMN02736856antibiotic_exposurenoR10.85biosample.attribute:geo loc nameVenezuela
SAMN02736856collection_date2009-04-25R10.90biosample.attribute:collection_date2009-04-25
SAMN02736856countryVER10.90sample.attr.geo_loc_nameVenezuela
SAMN02736856sexmaleR10.90sample.attr.host_sexmale
SAMN02736856subject_id3R10.85sample.attr.individual3
Sequencing

22 runs over 22 samples · mean 71.2 Mbp per run (median 38.9 Mbp, total 1.57 Gbp; 22 runs with a base count) · layout SINGLE 22 · platform ILLUMINA 22 · models NextSeq 500 (22) · 18% of runs with a Sandpiper profile · first public 2015-04-22

Authors (29)

Clemente JC first · Pehrsson EC · Blaser MJ · Sandhu K · Gao Z · Wang B · Magris M · Hidalgo G · Contreras M · Noya-Alarcón · Noya-Alarcón Ó · Lander O · McDonald J · Cox M · Walter J · Oh PL · Ruiz JF · Rodriguez S · Shen N · Song SJ · Metcalf J · Knight R · Dantas G · Dominguez-Bello MG last · Velsko IM first · Gallois S · Stahl R · Henry AG · Warinner C last

Downloads

PRJNA245336.csv.gz (1.6 KB) · parquet (70.2 KB) — all 22 samples, every field with route and confidence · PRJNA245336_determinations.csv.gz (1.5 KB) — 132 evidence rows

Samples (22 · every field this project has values for · hover a cell for its source)
Loading the sample table…