Gut microbiome in obese children on diet intervention, China
Shotgun metagenomic study of 109 fecal samples from Chinese children with Prader–Willi syndrome or simple obesity (38 subjects) before and after a non-digestible carbohydrate dietary intervention. machine-written summary
109 samples · 109 runs · CN · Child · 2015 · 7.53 Gbp / sample
Checked this project's papers and archive records yourself? Mark it human-verified.
what each source gave
| ✓ Sequence archive | 654 sample values (collection date, country, health condition, health condition detail, subject id, timepoint) |
| ○ Abstract / description | read; no usable statement |
| ✓ Full text | 109 sample values (location site) · PMC4563136 |
| ✓ Supplementary tables | 218 sample values (age, sex) |
| – External resources | not covered by the external resources we ingest |
| – User contribution | nothing uploaded yet |
| – Human verified | not yet checked by a person · I checked this project — verify it |
intervention — arms not resolved
Controlled / prescribed diet or feeding regimen design single_arm_before_after · population obesity · Hospitalized diet rich in non-digestible carbohydrates inducing weight loss
“a diet rich in non-digestible carbohydrates induced significant weight loss” — abstract:26425705 (study level; per-sample arms are in the intervention column below)
longitudinal — not resolved
37 of 38 people sampled more than once (108 samples; median 2, up to 4 per person). Schedule stated by the source: “Fecal samples collected at baseline (Day 0) and every 30 days during a hospitalized dietary intervention, up to Day 90 for PWS and Day 30 for simple obesity subjects.”; stated follow-up 3 mo.
No Sandpiper profile for this project's samples yet.
| field | samples | coverage | R1 | R2 | R3 | R4 |
|---|---|---|---|---|---|---|
| age_category | 109 | 100% | ||||
| country | 109 | 100% | 109 | |||
| health_condition | 109 | 100% | 109 | |||
| subject_id | 109 | 100% | 109 | |||
| age_at_collection_days | 109 | 100% | 109 | |||
| collection_date | 109 | 100% | 109 | |||
| detailed_location | 109 | 100% | ||||
| health_condition_detail | 109 | 100% | 109 | |||
| location_site | 109 | 100% | 109 | |||
| sex | 109 | 100% | 109 | |||
| timepoint_label | 109 | 100% | 109 |
- Dietary Modulation of Gut Microbiota Contributes to Alleviation of Both Genetic and Simple Obesity in Children. 2015 PMID 26425705 PMC4563136 own_data
Archive description
Gut microbiota has been causatively linked with diet-induced obesity however, its role in human genetic obesity remains elusive. Here we used bar-coded pyrosequencing of 16S rRNA gene V1-V3 region to show that dietary modulation of a structurally and functionally imbalanced gut microbiota significantly alleviates morbid obesity in children with Prader-Willi Syndrome.
How this project entered the registry
Classified as human gut / stool shotgun DNA metagenome of child by curator_audit with confidence 0.57. Assay shotgun_dna.
- host runs.scientific_name · “human gut metagenome (109)”
- host runs.environment_material · “feces (109)”
- body site runs.scientific_name · “human gut metagenome (109)”
- body site runs.environment_material · “feces (109)”
- life stage study.description · “morbid obesity in children with Prader-Willi”
Evidence examples (one per field × route)
| sample | field | value | route | conf. | source | quote |
|---|---|---|---|---|---|---|
| SAMN02929985 | age_at_collection_days | 5113.5 | R2 | 0.60 | paper.supp.mmc1.docx[table1!Age] | GD02 PWS male 14 |
| SAMN02929985 | collection_date | 2011 | R1 | 0.85 | biosample.attribute:collection_date | collection_date: 2011 |
| SAMN02929985 | country | CN | R1 | 0.90 | sample.attr.geo_loc_name | China: Guangdong |
| SAMN02929985 | health_condition | obesity | R1 | 0.85 | biosample.attribute:host_disease | host_disease: PWS |
| SAMN02929985 | health_condition_detail | Prader-Willi syndrome with morbid obesity (host_disease PWS) | R1 | 0.85 | biosample.attribute:host_disease | host_disease: PWS |
| SAMN02929985 | location_site | Guangdong Women and Children Hospital | R3 | 0.70 | paper.body.methods_results | hospitalized dietary intervention for 30 days in Guangdong Women and Children Hospital, Guangzhou, China |
| SAMN02929985 | sex | male | R2 | 0.75 | paper.supp.mmc1.docx[table1!Gender] | GD02 PWS male 14 |
| SAMN02929985 | subject_id | GD02 | R1 | 0.70 | sample.name_rule:library_name | GD0201 |
| SAMN02929985 | timepoint_label | 01 | R1 | 0.60 | sample.name_rule:library_name | GD0201 |
Sequencing
109 runs over 109 samples · mean 8.08 Gbp per run (median 7.53 Gbp, total 880.45 Gbp; 109 runs with a base count) · layout SINGLE 109 · platform ILLUMINA 109 · models Illumina HiSeq 2000 (109) · 85% of runs with a Sandpiper profile · first public 2015-07-26
Authors (0)
No author record.
Downloads
PRJNA256106.csv.gz (3.9 KB) · parquet (74.9 KB) — all 109 samples, every field with route and confidence · PRJNA256106_determinations.csv.gz (8.6 KB) — 981 evidence rows