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PRJNA273574 SRP053000

In vitro colonic fermentation of elderly gut microbiota

Intestinal model Metagenome

29 samples from Switzerland, derived from immobilized fecal microbiota of an elderly donor run in continuous colonic fermentation models (a three-stage model and the PolyFermS platform) simulating elderly gut fermentation. machine-written summary

29 samples · 29 runs · CH · Elderly · 2015 · 3.9 Mbp / sample

Sources
✓Sequence archive 174○Abstract / description✓Full text 116○Supplementary tables–External resources–User contribution–Human verified

Checked this project's papers and archive records yourself? Mark it human-verified.

what each source gave
✓ Sequence archive174 sample values (collection date, country, latitude, location region, longitude, subject id)
○ Abstract / descriptionread; no usable statement
✓ Full text116 sample values (antibiotic exposure, health condition, health condition detail, sex) · PMC4641611
○ Supplementary tablesfiles read; no table could be matched to the samples
– External resourcesnot covered by the external resources we ingest
– User contributionnothing uploaded yet
– Human verifiednot yet checked by a person · I checked this project — verify it
Samples at a glance
1 sampling locations · 1 country
Age
elderly29
Condition
healthy_control29
Sex
female29
Country
CH29
Body site
primary29
Collected 2012–2013
2012: 16 samples2013: 13 samples20122013
Sequencing depth per sample · this project all projects
all projects, 0–0.03 Gbp: 25,080 samples (4 %)this project, 0–0.03 Gbp: 29 samplesall projects, 0.03–0.1 Gbp: 17,066 samples (3 %)all projects, 0.1–0.3 Gbp: 29,179 samples (5 %)all projects, 0.3–1 Gbp: 47,044 samples (8 %)all projects, 1–3 Gbp: 124,983 samples (22 %)all projects, 3–10 Gbp: 253,728 samples (44 %)all projects, 10–30 Gbp: 72,533 samples (13 %)all projects, 30–100 Gbp: 3,024 samples (1 %)all projects, 100–300 Gbp: 106 samples (0 %)all projects, ≥ 300 Gbp: 9 samples (0 %)project median 0.00 Gbp per sample0130Gbp
Sampling design and relatedness

longitudinal — not resolved

2 of 2 people sampled more than once (29 samples; median 14, up to 16 per person).

How longitudinal and relatedness information is derived

Community composition

No Sandpiper profile for this project's samples yet.

Field coverage
fieldsamplescoverageR1R2R3R4
age_category29 100%
country29 100%29
health_condition29 100%29
subject_id29 100%29
antibiotic_exposure29 100%29
collection_date29 100%29
detailed_location29 100%
health_condition_detail29 100%29
latitude29 100%29
location_region29 100%29
longitude29 100%29
sex29 100%29
Papers (1)
  • Design and Investigation of PolyFermS In Vitro Continuous Fermentation Models Inoculated with Immobilized Fecal Microbiota Mimicking the Elderly Colon. 2015 PMID 26559530 PMC4641611 own_data
Details
Archive description

To study the microbiota of elderly using an intestinal fermentation model

How this project entered the registry

Classified as human gut / stool shotgun DNA metagenome of elderly by sonnet_x2 with confidence 0.55. Assay shotgun_dna.

  • host run.host_tax_id · “9606”
  • body site run.scientific_name · “gut metagenome”
  • life stage study.description · “microbiota of elderly using an intestinal fermentation model”
Evidence examples (one per field × route)
samplefieldvaluerouteconf.sourcequote
SAMN03294785antibiotic_exposurenoR30.70paper.fulltext.methodsdid not receive antibiotic treatment for at least three months
SAMN03294785collection_date2012R10.90biosample.attribute:collection_date2012
SAMN03294785countryCHR10.90sample.attr.geo_loc_nameSwitzerland
SAMN03294785health_conditionhealthy_controlR30.70paper.fulltext.methodsFecal samples were collected from three healthy women
SAMN03294785health_condition_detailhealthy womenR30.70paper.fulltext.methodsFecal samples were collected from three healthy women
SAMN03294785latitude47.3764R10.90biosample.attribute:lat_lon47.3764 N 8.5481 E
SAMN03294785location_regionZurichR10.60biosample.attribute:lat_lon47.3764 N 8.5481 E
SAMN03294785longitude8.5481R10.90biosample.attribute:lat_lon47.3764 N 8.5481 E
SAMN03294785sexfemaleR30.70paper.fulltext.methodsFecal samples were collected from three healthy women
SAMN03294785subject_idF2R10.70sample.name_rule:aliasF2-F
Sequencing

29 runs over 29 samples · mean 4.1 Mbp per run (median 3.9 Mbp, total 0.12 Gbp; 29 runs with a base count) · layout SINGLE 29 · platform LS454 29 · models 454 GS FLX Titanium (29) · 0% of runs with a Sandpiper profile · first public 2015-08-29

Authors (6)

Fehlbaum S first · Chassard C · Haug MC · Fourmestraux C · Derrien M · Lacroix C last

Downloads

PRJNA273574.csv.gz (1.4 KB) · parquet (70.9 KB) — all 29 samples, every field with route and confidence · PRJNA273574_determinations.csv.gz (2.4 KB) — 290 evidence rows

Samples (29 · every field this project has values for · hover a cell for its source)
Loading the sample table…