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PRJNA299342 SRP069299

SFB metagenome in infant intestinal samples, Hangzhou, China

children intestinal bacteria about SFB metagenome

11 samples from infants in Hangzhou, China, examining segmented filamentous bacteria (SFB) sequences to compare differences between mouse and human. Design not reported. machine-written summary

11 samples · 11 runs · CN · Infant / toddler · 2017 · 3.87 Gbp / sample

Sources
✓Sequence archive 22○Abstract / description✓Full text 11○Supplementary tables–External resources–User contribution–Human verified

Checked this project's papers and archive records yourself? Mark it human-verified.

what each source gave
✓ Sequence archive22 sample values (country, subject id)
○ Abstract / descriptionread; no usable statement
✓ Full text11 sample values (location locality) · PMC6034559
○ Supplementary tablesfiles read; no table could be matched to the samples
– External resourcesnot covered by the external resources we ingest
– User contributionnothing uploaded yet
– Human verifiednot yet checked by a person · I checked this project — verify it
Samples at a glance
1 country
Age
infant11
Country
CN11
Body site
unknown11
Sequencing depth per sample · this project all projects
all projects, 0–0.03 Gbp: 25,080 samples (4 %)all projects, 0.03–0.1 Gbp: 17,066 samples (3 %)all projects, 0.1–0.3 Gbp: 29,179 samples (5 %)all projects, 0.3–1 Gbp: 47,044 samples (8 %)all projects, 1–3 Gbp: 124,983 samples (22 %)all projects, 3–10 Gbp: 253,728 samples (44 %)this project, 3–10 Gbp: 11 samplesall projects, 10–30 Gbp: 72,533 samples (13 %)all projects, 30–100 Gbp: 3,024 samples (1 %)all projects, 100–300 Gbp: 106 samples (0 %)all projects, ≥ 300 Gbp: 9 samples (0 %)project median 3.87 Gbp per sample0130Gbp
Sampling design and relatedness

longitudinal — not resolved

4 of 6 people sampled more than once (9 samples; median 2, up to 3 per person).

How longitudinal and relatedness information is derived

Community composition
Bacteroides 21.9%Phocaeicola 20.7%Faecalibacterium 8.4%Roseburia 4.9%Ruminococcoides 3.1%Fusobacterium_A 2.8%Alistipes 2.6%Lachnospira 2.6%Dialister 2.1%Veillonella 1.7%Prevotella 1.6%CAG-103 1.3%not assigned to a genus 4.9%other genera 21.4%

Genera, mean relative abundance over 10 profiled gut samples of this project (Sandpiper SingleM profiles, GTDB R232; low-depth and non-metagenome profiles left out).

Field coverage
fieldsamplescoverageR1R2R3R4
age_category11 100%
country11 100%11
health_condition0 0%
subject_id11 100%11
detailed_location11 100%
location_locality11 100%11
Papers (2)
  • Genome sequence of segmented filamentous bacteria present in the human intestine. 2020 PMID 32887924 PMC7474095 cites_accession
  • Presence of Segmented Filamentous Bacteria in Human Children and Its Potential Role in the Modulation of Human Gut Immunity. 2018 PMID 30008704 PMC6034559 own_data
Details
Archive description

To find out the differences of SFB sequences between mouse and human

How this project entered the registry

Classified as human gut / stool shotgun DNA metagenome of infant / toddler and child by deterministic_prior with confidence 0.90. Assay shotgun_dna.

  • host external_curation.infant_catalog · “infant triage verdict include”
  • body site study.title · “children intestinal bacteria about SFB”
  • body site external_curation.infant_catalog · “infant triage verdict include (gut/stool scope)”
  • life stage external_curation.infant_catalog · “infant triage verdict include (0-3 y scope)”
  • life stage study.title · “children intestinal bacteria about”
Evidence examples (one per field × route)
samplefieldvaluerouteconf.sourcequote
SRR3147679countryCNR10.90sample.attr.geo_loc_nameChina:Hangzhou
SRR3147679location_localityHangzhouR30.70paper.body.text144 individuals in Hangzhou, China (80 males, 64 females; 2 months to 178 months-of-age) were enrolled in our SFB study
SRR3147679subject_id1R10.70sample.name_rule:library_nameA1-2
Sequencing

11 runs over 11 samples · mean 4.03 Gbp per run (median 3.87 Gbp, total 44.36 Gbp; 11 runs with a base count) · layout PAIRED 11 · platform ILLUMINA 11 · models Illumina HiSeq 2000 (11) · 91% of runs with a Sandpiper profile · first public 2017-02-05

Authors (0)

No author record.

Downloads

PRJNA299342.csv.gz (1.0 KB) · parquet (68.8 KB) — all 11 samples, every field with route and confidence · PRJNA299342_determinations.csv.gz (828 B) — 33 evidence rows

Samples (11 · every field this project has values for · hover a cell for its source)
Loading the sample table…