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PRJNA321218 SRP074801

Human gut microbiome in healthy adults, cross-sectional

human gut and groundwater harbor non-photosynthetic bacteria

3 gut samples from healthy adult controls (country not reported), collected in a cross-sectional design; part of a study describing a new candidate bacterial phylum found in human gut and groundwater. machine-written summary

3 samples · 3 runs · GB · Adult · 2016 · 5.48 Gbp / sample

Sources
✓Sequence archive 9○Abstract / description✓Full text 3○Supplementary tables–External resources–User contribution–Human verified

Checked this project's papers and archive records yourself? Mark it human-verified.

what each source gave
✓ Sequence archive9 sample values (health condition, health condition detail, subject id)
○ Abstract / descriptionread; no usable statement
✓ Full text3 sample values (country) · PMC3787301, PMC5538567
○ Supplementary tablesfiles read; no table could be matched to the samples
– External resourcesnot covered by the external resources we ingest
– User contributionnothing uploaded yet
– Human verifiednot yet checked by a person · I checked this project — verify it
Samples at a glance
1 country
Age
adult3
Condition
healthy_control3
Country
GB3
Body site
primary3
Sequencing depth per sample · this project all projects
all projects, 0–0.03 Gbp: 25,080 samples (4 %)all projects, 0.03–0.1 Gbp: 17,066 samples (3 %)all projects, 0.1–0.3 Gbp: 29,179 samples (5 %)all projects, 0.3–1 Gbp: 47,044 samples (8 %)all projects, 1–3 Gbp: 124,983 samples (22 %)all projects, 3–10 Gbp: 253,728 samples (44 %)this project, 3–10 Gbp: 3 samplesall projects, 10–30 Gbp: 72,533 samples (13 %)all projects, 30–100 Gbp: 3,024 samples (1 %)all projects, 100–300 Gbp: 106 samples (0 %)all projects, ≥ 300 Gbp: 9 samples (0 %)project median 5.48 Gbp per sample0130Gbp
Community composition
Prevotella 16.3%Bacteroides 14.2%Phocaeicola 9.0%Faecalibacterium 4.5%Ruminiclostridium_E 3.0%Acetatifactor 2.7%Alistipes 2.5%Roseburia 2.3%Blautia_A 2.3%Lachnospira 2.2%CAG-307 1.8%Vescimonas 1.6%not assigned to a genus 5.3%other genera 32.5%

Genera, mean relative abundance over 3 profiled gut samples of this project (Sandpiper SingleM profiles, GTDB R232; low-depth and non-metagenome profiles left out).

Field coverage
fieldsamplescoverageR1R2R3R4
age_category3 100%
country3 100%3
health_condition3 100%3
subject_id3 100%3
health_condition_detail3 100%3
Papers (2)
  • The human gut and groundwater harbor non-photosynthetic bacteria belonging to a new candidate phylum sibling to Cyanobacteria. 2013 PMID 24137540 PMC3787301 own_data
  • Measurement of bacterial replication rates in microbial communities. 2016 PMID 27819664 PMC5538567 own_data
Details
Archive description

S. C. Di Rienzi, I. Sharon, K. C. Wrighton, O. Koren, L. A. Hug, B. C. Thomas, J. K. Goodrich, J. T. Bell, T. D. Spector, J. F. Banfield, and R. E. Ley, “The human gut and groundwater harbor non-photosynthetic bacteria belonging to a new candidate phylum sibling to Cyanobacteria.,” Elife, vol. 2, pp. e01102–e01102, Jan. 2013.

How this project entered the registry

Classified as human gut / stool shotgun DNA metagenome of adult by deterministic_prior with confidence 0.90. Assay shotgun_dna.

  • host external_curation.infant_catalog · “infant triage reason_code age_adult_only”
  • body site study.title · “human gut and groundwater harbor”
  • body site study.description · “Ley, “The human gut and groundwater harbor”
  • life stage external_curation.infant_catalog · “infant triage reason_code age_adult_only”
Evidence examples (one per field × route)
samplefieldvaluerouteconf.sourcequote
SAMN04978191countryGBR30.70paper.fulltext.methodsUnited Kingdom Adult Twin Registry (TwinsUK)
SAMN04978191health_conditionhealthy_controlR10.85biosample.attribute:aliasalias: fecal sample 1 from healthy adult A
SAMN04978191health_condition_detailfecal sample 1 from healthy adult AR10.85biosample.attribute:aliasalias: fecal sample 1 from healthy adult A
SAMN04978191subject_idadult AR10.85biosample.attribute:DESCRIPTIONsample from healthy adult A
Sequencing

3 runs over 3 samples · mean 5.57 Gbp per run (median 5.48 Gbp, total 16.70 Gbp; 3 runs with a base count) · layout PAIRED 3 · platform ILLUMINA 3 · models Illumina HiSeq 2000 (3) · 100% of runs with a Sandpiper profile · first public 2016-05-17

Authors (33)

Di Rienzi SC first · Sharon I · Wrighton KC · Koren O · Hug LA · Thomas BC · Goodrich JK · Bell JT · Spector TD · Banfield JF · Ley RE last · Brown CT first · Olm MR · Fontana F first · Alessandri G · Tarracchini C · Bianchi MG · Rizzo SM · Mancabelli L · Lugli GA · Argentini C · Vergna LM · Anzalone R · Longhi G · Viappiani A · Taurino G · Chiu M · Turroni F · Bussolati O · van Sinderen D · Milani C · Ventura M last · Angelini L

Downloads

PRJNA321218.csv.gz (954 B) · parquet (69.0 KB) — all 3 samples, every field with route and confidence · PRJNA321218_determinations.csv.gz (720 B) — 12 evidence rows

Samples (3 · every field this project has values for · hover a cell for its source)
Loading the sample table…