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PRJNA340216 SRP083099

Gut metagenomics, omnivore, vegetarian, vegan adults, Italy

Gut microbiota and metagenomic diversity of omnivore, vegetarian and vegan healthy subjects

Cross-sectional study of 27 fecal samples from healthy adult omnivorous, vegetarian and vegan volunteers in Parma, Bari, Torino and Bologna, Italy, examining gut microbial functional diversity by diet type. machine-written summary

27 samples · 27 runs · IT · Age unknown · 2016 · 6.71 Gbp / sample

Sources
✓Sequence archive 156○Abstract / description✓Full text 35✓Supplementary tables 6✓External resources 115–User contribution–Human verified

Checked this project's papers and archive records yourself? Mark it human-verified.

what each source gave
✓ Sequence archive156 sample values (collection date, country, diet, location locality, subject id, timepoint)
○ Abstract / descriptionread; no usable statement
✓ Full text35 sample values (antibiotic exposure, health condition, health condition detail) · PMC12199706, PMC7060259, PMC8634118
✓ Supplementary tables6 sample values (subject id)
✓ External resources115 sample values (age, bmi, health condition, health condition detail, sex) · from curatedMetagenomicData 3 (cMD 3)
– User contributionnothing uploaded yet
– Human verifiednot yet checked by a person · I checked this project — verify it
Samples at a glance
1 country
Age
adult27
Condition
healthy_control27
Sex
female15
male8
Country
IT27
Body site
primary27
Sequencing depth per sample · this project all projects
all projects, 0–0.03 Gbp: 25,080 samples (4 %)all projects, 0.03–0.1 Gbp: 17,066 samples (3 %)all projects, 0.1–0.3 Gbp: 29,179 samples (5 %)all projects, 0.3–1 Gbp: 47,044 samples (8 %)all projects, 1–3 Gbp: 124,983 samples (22 %)this project, 1–3 Gbp: 1 samplesall projects, 3–10 Gbp: 253,728 samples (44 %)this project, 3–10 Gbp: 26 samplesall projects, 10–30 Gbp: 72,533 samples (13 %)all projects, 30–100 Gbp: 3,024 samples (1 %)all projects, 100–300 Gbp: 106 samples (0 %)all projects, ≥ 300 Gbp: 9 samples (0 %)project median 6.71 Gbp per sample0130Gbp
Community composition
Prevotella 6.6%Faecalibacterium 5.9%Bacteroides 5.5%Phocaeicola 5.4%Bifidobacterium 4.9%Roseburia 4.8%Alistipes 3.2%Ruminococcoides 3.2%Vescimonas 2.9%CAG-177 2.6%Blautia_A 2.4%Lachnospira 2.2%not assigned to a genus 5.8%other genera 44.5%

Genera, mean relative abundance over 25 profiled gut samples of this project (Sandpiper SingleM profiles, GTDB R232; low-depth and non-metagenome profiles left out).

Field coverage
fieldsamplescoverageR1R2R3R4
age_category27 100%
country27 100%27
health_condition27 100%234
subject_id27 100%216
antibiotic_exposure27 100%27
collection_date27 100%27
detailed_location27 100%
diet27 100%27
health_condition_detail27 100%234
location_locality27 100%27
timepoint_label27 100%27
age_at_collection_days23 85%23
bmi23 85%23
sex23 85%23
Papers (3)
  • Diet influences the functions of the human intestinal microbiome. 2020 PMID 32144387 PMC7060259 own_data
  • A versatile genetic toolbox for Prevotella copri enables studying polysaccharide utilization systems. 2021 PMID 34676563 PMC8634118 unsure
  • Integrating comparative genomics and risk classification by assessing virulence, antimicrobial resistance, and plasmid spread in microbial communities with gSpreadComp. 2025 PMID 40569694 PMC12199706 unsure
Details
Archive description

Gut microbiota and metagenomic diversity of omnivore, vegetarian and vegan healthy subjects

How this project entered the registry

Classified as human gut / stool shotgun DNA metagenome of age unknown by sonnet_x2 with confidence 0.72. Assay shotgun_dna.

  • host run.scientific_name · “human gut metagenome”
  • body site study.title · “Gut microbiota and metagenomic diversity”
Evidence examples (one per field × route)
samplefieldvaluerouteconf.sourcequote
SAMN05710166age_at_collection_days12418.5R20.75external.cmd sourceage: 34; age_unit: Year; age_group: Adult
SAMN05710166antibiotic_exposurenoR30.70paper.fulltext.methodsconsumption of antibiotics in the previous 3 months
SAMN05710166bmi21.5R20.75external.cmd sourcebmi: 21.5
SAMN05710166collection_date2013R10.90biosample.attribute:collection_date2013
SAMN05710166countryITR10.90sample.attr.geo_loc_nameItaly:Bari
SAMN05710166dietvegetarianR10.85biosample.attribute:titlefeces from vegetarian subject from Bari 01
SAMN05710171dietveganR10.70sample.name_rule:alias07.PR.V.2
SAMN05710166health_conditionhealthy_controlR20.75external.cmd sourcedisease: Healthy
SAMN05710172health_conditionhealthy_controlR30.70paper.fulltext.methodsThirty healthy adult volunteers
SAMN05710166health_condition_detailHealthyR20.75external.cmd sourcedisease: Healthy
SAMN05710172health_condition_detailhealthy adult volunteersR30.70paper.fulltext.methodsThirty healthy adult volunteers
SAMN05710166location_localityBariR10.85biosample.attribute:geo_loc_nameItaly:Bari
SAMN05710166sexmaleR20.75external.cmd sourcesex: Male
SAMN05710166subject_id01R10.70sample.name_rule:alias01.BA.VG.2
SAMN05710167subject_idBari 04R20.80archive
SAMN05710166timepoint_label2R10.70sample.name_rule:alias01.BA.VG.2
Sequencing

27 runs over 27 samples · mean 6.62 Gbp per run (median 6.71 Gbp, total 178.66 Gbp; 27 runs with a base count) · layout SINGLE 27 · platform ILLUMINA 27 · models Illumina HiSeq 1500 (27) · 93% of runs with a Sandpiper profile · first public 2016-09-05 – 2016-09-07

Authors (55)

De Angelis M first · Ferrocino I · Calabrese FM · De Filippis F · Cavallo N · Siragusa S · Rampelli S · Di Cagno R · Rantsiou K · Vannini L · Pellegrini N · Lazzi C · Turroni S · Lorusso N · Ventura M · Chieppa M · Neviani E · Brigidi P · O'Toole PW · Ercolini D · Gobbetti M · Cocolin L last · Li J first · Gálvez EJC · Amend L · Almási · Iljazovic A · Lesker TR · Bielecka AA · Schorr EM · Strowig T last · Argentini C first · Lugli GA · Tarracchini C · Fontana F · Mancabelli L · Viappiani A · Anzalone R · Angelini L · Alessandri G · Longhi G · Bianchi MG · Taurino G · Bussolati O · Milani C · van Sinderen D · Turroni F · Kasmanas JC first · Magnúsdóttir S · Zhang J · Smalla K · Schloter M · Stadler PF · de Leon Ferreira de Carvalho ACP · Rocha U last

Downloads

PRJNA340216.csv.gz (2.0 KB) · parquet (72.0 KB) — all 27 samples, every field with route and confidence · PRJNA340216_determinations.csv.gz (4.9 KB) — 312 evidence rows

Samples (27 · every field this project has values for · hover a cell for its source)
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