Home › Studies › PRJNA413356
PRJNA413356 SRP119685

Stool microbiome in TB/H. pylori breath-test study, Mali

Mycobacterium tuberculosis Genome sequencing and assembly

Shotgun metagenomic analysis of stool from 10 samples collected in Mali as part of a study comparing oral urease breath test results with tuberculosis and Helicobacter pylori status. machine-written summary

10 samples · 10 runs · ML · Age unknown · 2017 · 18.06 Gbp / sample

Sources
✓Sequence archive 10✓Abstract / description 10✓Full text 50–Supplementary tables–External resources–User contribution–Human verified

Checked this project's papers and archive records yourself? Mark it human-verified.

what each source gave
✓ Sequence archive10 sample values (country)
✓ Abstract / description10 sample values (location region)
✓ Full text50 sample values (collection date, health condition, health condition detail, location locality, location site) · PMC5046223
– Supplementary tablesno supplementary tables available (or none open access)
– External resourcesnot covered by the external resources we ingest
– User contributionnothing uploaded yet
– Human verifiednot yet checked by a person · I checked this project — verify it
Samples at a glance
1 country
Age
adult10
Condition
healthy_control10
Country
ML10
Body site
primary10
Sequencing depth per sample · this project all projects
all projects, 0–0.03 Gbp: 25,080 samples (4 %)all projects, 0.03–0.1 Gbp: 17,066 samples (3 %)all projects, 0.1–0.3 Gbp: 29,179 samples (5 %)all projects, 0.3–1 Gbp: 47,044 samples (8 %)all projects, 1–3 Gbp: 124,983 samples (22 %)all projects, 3–10 Gbp: 253,728 samples (44 %)this project, 3–10 Gbp: 1 samplesall projects, 10–30 Gbp: 72,533 samples (13 %)this project, 10–30 Gbp: 9 samplesall projects, 30–100 Gbp: 3,024 samples (1 %)all projects, 100–300 Gbp: 106 samples (0 %)all projects, ≥ 300 Gbp: 9 samples (0 %)project median 18.06 Gbp per sample0130Gbp
Community composition

No Sandpiper profile for this project's samples yet.

Field coverage
fieldsamplescoverageR1R2R3R4
age_category10 100%
country10 100%10
health_condition10 100%10
subject_id0 0%
collection_date10 100%10
detailed_location10 100%
health_condition_detail10 100%10
location_locality10 100%10
location_region10 100%10
location_site10 100%10
Papers (1)
  • Stool microbiome reveals diverse bacterial ureases as confounders of oral urea breath testing for Helicobacter pylori and Mycobacterium tuberculosis in Bamako, Mali. 2016 PMID 27532494 PMC5046223 own_data
Details
Archive description

Genome sequencing of M.tb

How this project entered the registry

Classified as human gut / stool shotgun DNA metagenome of age unknown by curator_audit with confidence 0.50. Assay shotgun_dna.

  • host runs.scientific_name · “human gut metagenome (10)”
  • host runs.isolation_source · “feces (10)”
  • body site runs.scientific_name · “human gut metagenome (10)”
  • body site runs.isolation_source · “feces (10)”
Evidence examples (one per field × route)
samplefieldvaluerouteconf.sourcequote
SAMN07740643collection_date2011-06/2012-12R30.70paper.fulltext.methodsFrom June of 2011 to December 2012
SAMN07740643countryMLR10.90sample.attr.geo_loc_nameMali
SAMN07740643health_conditionhealthy_controlR30.60paper.fulltext.resultshealthy control participants with no clinical or laboratory evidence
SAMN07740643health_condition_detailTB- and H. pylori- healthy controls with positive oral UBT, stool sequencedR30.60paper.fulltext.resultshealthy control participants with no clinical or laboratory evidence
SAMN07740643location_localityBamakoR30.70paper.fulltext.methodssix referring health centers of Bamako and Point-G Hospital of Bamako
SAMN07740643location_regionBamakoR40.50paper.abstracthealthy subjects in Bamako, Mali and performed
SAMN07740643location_sitePoint-G Hospital of BamakoR30.70paper.fulltext.methodsPoint-G Hospital of Bamako, Mali
Sequencing

10 runs over 10 samples · mean 17.08 Gbp per run (median 18.06 Gbp, total 170.79 Gbp; 10 runs with a base count) · layout PAIRED 10 · platform ILLUMINA 10 · models Illumina HiSeq 2000 (10) · 80% of runs with a Sandpiper profile · first public 2017-10-11 – 2017-10-17

Authors (23)

Maiga M first · Cohen K · Baya B · Srikrishna G · Siddiqui S · Sanogo M · Somboro AM · Diarra B · Diallo MH · Mazumdar V · Yoder C · Orsega S · Belson M · Kassambara H · Goita D · Murphy RL · Dao S · Polis M · Diallo S · Timmins GS · Dodd L · Earl AM · Bishai WR last

Downloads

PRJNA413356.csv.gz (1.2 KB) · parquet (70.4 KB) — all 10 samples, every field with route and confidence · PRJNA413356_determinations.csv.gz (1.1 KB) — 70 evidence rows

Samples (10 · every field this project has values for · hover a cell for its source)
Loading the sample table…