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PRJNA415207 SRP120619

Viral metagenome of children's stool samples, Ethiopia

Viral metagenome of children stool sample from Ethiopia

29 stool samples from healthy children in Amhara, Ethiopia, analyzed by viral metagenomics; curated records indicate an intervention-based study design. machine-written summary

29 samples · 29 runs · ET · Child · 2017 · 0.14 Gbp / sample

Sources
✓Sequence archive 145○Abstract / description✓Full text 58○Supplementary tables✓External resources 58–User contribution–Human verified

Checked this project's papers and archive records yourself? Mark it human-verified.

what each source gave
✓ Sequence archive145 sample values (collection date, country, latitude, location region, longitude)
○ Abstract / descriptionread; no usable statement
✓ Full text58 sample values (lifestyle, lifestyle detail) · PMC6095524, PMC6832430
○ Supplementary tablesfiles read; no table could be matched to the samples
✓ External resources58 sample values (health condition, health condition detail) · from African Human Microbiome Portal (H3ABioNet)
– User contributionnothing uploaded yet
– Human verifiednot yet checked by a person · I checked this project — verify it
Interventions

intervention — arms not resolved

Other administered intervention (see intervention_detail) design observational_with_procedure · population healthy_control · New hand-dug water well (clean water intervention)

“clean water intervention trial” — abstract:30114205 (study level; per-sample arms are in the intervention column below)

Samples at a glance
1 sampling locations · 1 country
Age
child29
Condition
healthy_control29
Country
ET29
Body site
primary29
Sequencing depth per sample · this project all projects
all projects, 0–0.03 Gbp: 25,080 samples (4 %)this project, 0–0.03 Gbp: 1 samplesall projects, 0.03–0.1 Gbp: 17,066 samples (3 %)this project, 0.03–0.1 Gbp: 4 samplesall projects, 0.1–0.3 Gbp: 29,179 samples (5 %)this project, 0.1–0.3 Gbp: 21 samplesall projects, 0.3–1 Gbp: 47,044 samples (8 %)this project, 0.3–1 Gbp: 3 samplesall projects, 1–3 Gbp: 124,983 samples (22 %)all projects, 3–10 Gbp: 253,728 samples (44 %)all projects, 10–30 Gbp: 72,533 samples (13 %)all projects, 30–100 Gbp: 3,024 samples (1 %)all projects, 100–300 Gbp: 106 samples (0 %)all projects, ≥ 300 Gbp: 9 samples (0 %)project median 0.14 Gbp per sample0130Gbp
Community composition
Prevotella 53.3%Faecalibacterium 1.4%Ruminococcoides 1.2%Cryptobacteroides 0.4%Vescimonas 0.3%Enterousia 0.3%not assigned to a genus 43.2%

Genera, mean relative abundance over 13 profiled gut samples of this project (Sandpiper SingleM profiles, GTDB R232; low-depth and non-metagenome profiles left out).

Field coverage
fieldsamplescoverageR1R2R3R4
age_category29 100%
country29 100%29
health_condition29 100%29
subject_id0 0%
collection_date29 100%29
detailed_location29 100%
health_condition_detail29 100%29
latitude29 100%29
lifestyle29 100%29
lifestyle_detail29 100%29
location_region29 100%29
longitude29 100%29
Papers (2)
Details
Archive description

Viral metagenome of children stool sample from Ethiopia

How this project entered the registry

Classified as human gut / stool shotgun DNA metagenome of child by deterministic_rule with confidence 0.90. Assay shotgun_dna.

  • host run.host_tax_id · “host_tax_id 9606”
  • body site study.title · “metagenome of children stool sample from Ethiopia”
  • body site study.description · “metagenome of children stool sample from Ethiopia”
  • life stage study.title · “Viral metagenome of children stool sample from”
  • life stage study.description · “Viral metagenome of children stool sample from”
Evidence examples (one per field × route)
samplefieldvaluerouteconf.sourcequote
SAMN07818838collection_date2016-04-01R10.90biosample.attribute:collection_date2016-04-01
SAMN07818838countryETR10.90sample.attr.geo_loc_nameEthiopia: Amhara
SAMN07818838health_conditionhealthy_controlR20.70external.ahmp sourceDISEASE: Healthy
SAMN07818838health_condition_detailHealthyR20.70external.ahmp sourceDISEASE: Healthy
SAMN07818838latitude11.3900R10.90biosample.attribute:lat_lon11.39 N 37.57 E
SAMN07818838lifestylerural_non_industrializedR30.70paper.fulltext.methodsrural agrarian region in the Goncha Siso Enese district
SAMN07818838lifestyle_detailrural agrarian children, Amhara EthiopiaR30.70paper.fulltext.methodsrural agrarian region in the Goncha Siso Enese district
SAMN07818838location_regionAmharaR10.85biosample.attribute:geo_loc_nameEthiopia: Amhara
SAMN07818838longitude37.5700R10.90biosample.attribute:lat_lon11.39 N 37.57 E
Sequencing

29 runs over 29 samples · mean 0.17 Gbp per run (median 0.14 Gbp, total 4.92 Gbp; 29 runs with a base count) · layout PAIRED 29 · platform ILLUMINA 29 · models Illumina MiSeq (29) · 69% of runs with a Sandpiper profile · first public 2017-10-21

Authors (14)

Altan E first · Aiemjoy K · Phan TG · Deng X · Aragie S · Tadesse Z · Callahan KE · Keenan J · Delwart E last · Li Y · Sabino-Santos G · Sawaswong V · Barnum S · Pusterla N

Downloads

PRJNA415207.csv.gz (1.6 KB) · parquet (71.1 KB) — all 29 samples, every field with route and confidence · PRJNA415207_determinations.csv.gz (2.6 KB) — 261 evidence rows

Samples (29 · every field this project has values for · hover a cell for its source)
Loading the sample table…