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PRJNA427187 SRP127558

Virome of breast milk and infant feces, USA

Maternal Milk Infant Fecal Virome Study

Viral metagenomics of 20 samples from mother-infant pairs in the USA comparing breast milk and infant fecal viromes to assess phage transmission to the infant gut. machine-written summary

20 samples · 20 runs · US · Mixed ages · 2018 · 1.36 Gbp / sample

Sources
✓Sequence archive 100○Abstract / description○Full text✓Supplementary tables 60–External resources–User contribution–Human verified

Checked this project's papers and archive records yourself? Mark it human-verified.

what each source gave
✓ Sequence archive100 sample values (collection date, country, family id, family role, subject id)
○ Abstract / descriptionread; no usable statement
○ Full textread; no usable statement or table · PMC5992295
✓ Supplementary tables60 sample values (age, antibiotic exposure, sex)
– External resourcesnot covered by the external resources we ingest
– User contributionnothing uploaded yet
– Human verifiednot yet checked by a person · I checked this project — verify it
Samples at a glance
1 country
Age
adult10
neonate10
Sex
female13
male7
Country
US20
Body site
excluded10
primary10
Sequencing depth per sample · this project all projects
all projects, 0–0.03 Gbp: 25,080 samples (4 %)all projects, 0.03–0.1 Gbp: 17,066 samples (3 %)all projects, 0.1–0.3 Gbp: 29,179 samples (5 %)all projects, 0.3–1 Gbp: 47,044 samples (8 %)this project, 0.3–1 Gbp: 3 samplesall projects, 1–3 Gbp: 124,983 samples (22 %)this project, 1–3 Gbp: 17 samplesall projects, 3–10 Gbp: 253,728 samples (44 %)all projects, 10–30 Gbp: 72,533 samples (13 %)all projects, 30–100 Gbp: 3,024 samples (1 %)all projects, 100–300 Gbp: 106 samples (0 %)all projects, ≥ 300 Gbp: 9 samples (0 %)project median 1.36 Gbp per sample0130Gbp
Sampling design and relatedness

mother–infant

10 mother–infant pairs: infant stool (Baby<n>) and maternal breast milk (Mother<n>) · 10 families with 20 sampled people (10 with a parent and a child).

motherMchildC
10 × mother + child
family pair1Baby1 — child · 1 sampleCBaby1Mother1 — mother · 1 sampleMMothe…
family pair10Baby10 — child · 1 sampleCBaby10Mother10 — mother · 1 sampleMMothe…
family pair2Baby2 — child · 1 sampleCBaby2Mother2 — mother · 1 sampleMMothe…
family pair3Baby3 — child · 1 sampleCBaby3Mother3 — mother · 1 sampleMMothe…
family pair4Baby4 — child · 1 sampleCBaby4Mother4 — mother · 1 sampleMMothe…
family pair5Baby5 — child · 1 sampleCBaby5Mother5 — mother · 1 sampleMMothe…
family pair6Baby6 — child · 1 sampleCBaby6Mother6 — mother · 1 sampleMMothe…
family pair7Baby7 — child · 1 sampleCBaby7Mother7 — mother · 1 sampleMMothe…
family pair8Baby8 — child · 1 sampleCBaby8Mother8 — mother · 1 sampleMMothe…
family pair9Baby9 — child · 1 sampleCBaby9Mother9 — mother · 1 sampleMMothe…

motherchild · circles are people (pale = no sequenced sample), arrows run donor → recipient and parent → child, curved lines join twins, partners and siblings, squares are shared settings or families without recorded roles. Shown: 10 families. 10 people in the project overall.

Families (10)
familymemberssamples
pair1mother + childSAMN08225420 · SAMN08225430
pair10mother + childSAMN08225429 · SAMN08225439
pair2mother + childSAMN08225421 · SAMN08225431
pair3mother + childSAMN08225422 · SAMN08225432
pair4mother + childSAMN08225423 · SAMN08225433
pair5mother + childSAMN08225424 · SAMN08225434
pair6mother + childSAMN08225425 · SAMN08225435
pair7mother + childSAMN08225426 · SAMN08225436
pair8mother + childSAMN08225427 · SAMN08225437
pair9mother + childSAMN08225428 · SAMN08225438

How longitudinal and relatedness information is derived

Community composition

No Sandpiper profile for this project's samples yet.

Field coverage
fieldsamplescoverageR1R2R3R4
age_category20 100%
country20 100%20
health_condition0 0%
subject_id20 100%20
age_at_collection_days20 100%20
antibiotic_exposure20 100%20
collection_date20 100%20
family_id20 100%20
family_role20 100%20
sex20 100%20
Papers (1)
Details
Archive description

Characterization of shared characteristics between viromes of maternal milk and infant feces

How this project entered the registry

Classified as human gut / stool / human milk / multiple sites shotgun DNA metagenome of infant / toddler and adult by curator_audit with confidence 0.55. Assay shotgun_dna.

  • host runs.isolation_source · “feces (10); Breastmilk (10)”
  • host runs.scientific_name · “human gut metagenome (10)”
  • body site runs.isolation_source · “feces (10); Breastmilk (10)”
  • body site runs.scientific_name · “human gut metagenome (10)”
  • life stage study.title · “Maternal Milk Infant Fecal Virome Study”
Evidence examples (one per field × route)
samplefieldvaluerouteconf.sourcequote
SAMN08225420age_at_collection_days9.0R20.85paper.supp.Table_1.DOCX[table1!Age]Baby1 BB1 9 days M antibiotics No
SAMN08225420antibiotic_exposurenoR20.85paper.supp.Table_1.DOCX[table1!Antibiotics]Baby1 BB1 9 days M antibiotics No
SAMN08225420collection_date2016-06-16R10.85biosample.attribute:collection_datecollection_date: 2016-06-16
SAMN08225420countryUSR10.90sample.attr.geo_loc_nameUSA: California
SAMN08225420family_idpair1R10.70sample.name_rule:TITLETITLE: Baby1; isolation_source: feces
SAMN08225420family_rolechildR10.70sample.name_rule:TITLETITLE: Baby1; isolation_source: feces
SAMN08225420sexmaleR20.85paper.supp.Table_1.DOCX[table1!Sex]Baby1 BB1 9 days M antibiotics No
SAMN08225420subject_idBaby1R10.70sample.name_rule:titleBaby1
Sequencing

20 runs over 20 samples · mean 1.37 Gbp per run (median 1.36 Gbp, total 27.35 Gbp; 20 runs with a base count) · layout PAIRED 20 · platform ILLUMINA 20 · models Illumina MiniSeq (20) · 30% of runs with a Sandpiper profile · first public 2018-11-14

Authors (0)

No author record.

Downloads

PRJNA427187.csv.gz (1.6 KB) · parquet (70.7 KB) — all 20 samples, every field with route and confidence · PRJNA427187_determinations.csv.gz (2.6 KB) — 160 evidence rows

Samples (20 · every field this project has values for · hover a cell for its source)
Loading the sample table…