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PRJNA489693 SRP160134

NICU infant feeding tube microbiota, USA (Davis)

Microbial Diversity of NICU Infant Feeding Tubes

18 samples from feeding tube segments, residual feeding tube contents, and feces of infants/neonates in a NICU in Davis, USA; case series design, with 16S rRNA sequencing and metagenomic sequencing on a small subset. machine-written summary

18 samples · 18 runs · US · Infant / toddler · 2019 · 3.33 Gbp / sample

Sources
✓Sequence archive 54○Abstract / description○Full text–Supplementary tables–External resources–User contribution–Human verified

Checked this project's papers and archive records yourself? Mark it human-verified.

what each source gave
✓ Sequence archive54 sample values (age, country, subject id)
○ Abstract / descriptionread; no usable statement
○ Full textread; no usable statement or table · PMC10269646
– Supplementary tablesno supplementary tables available (or none open access)
– External resourcesnot covered by the external resources we ingest
– User contributionnothing uploaded yet
– Human verifiednot yet checked by a person · I checked this project — verify it
Samples at a glance
1 country
Age
infant12
neonate6
Country
US18
Body site
excluded12
primary6
Sequencing depth per sample · this project all projects
all projects, 0–0.03 Gbp: 25,080 samples (4 %)all projects, 0.03–0.1 Gbp: 17,066 samples (3 %)all projects, 0.1–0.3 Gbp: 29,179 samples (5 %)this project, 0.1–0.3 Gbp: 2 samplesall projects, 0.3–1 Gbp: 47,044 samples (8 %)this project, 0.3–1 Gbp: 2 samplesall projects, 1–3 Gbp: 124,983 samples (22 %)this project, 1–3 Gbp: 5 samplesall projects, 3–10 Gbp: 253,728 samples (44 %)this project, 3–10 Gbp: 9 samplesall projects, 10–30 Gbp: 72,533 samples (13 %)all projects, 30–100 Gbp: 3,024 samples (1 %)all projects, 100–300 Gbp: 106 samples (0 %)all projects, ≥ 300 Gbp: 9 samples (0 %)project median 3.33 Gbp per sample0130Gbp
Community composition
Klebsiella 24.2%Enterobacter 16.5%Escherichia 14.4%Veillonella 11.2%Sarcina 9.5%Citrobacter 6.3%Enterococcus 2.5%Cutibacterium 1.8%Streptococcus 1.6%Staphylococcus 1.3%Clostridium_F 0.9%Finegoldia 0.9%not assigned to a genus 3.8%other genera 5.1%

Genera, mean relative abundance over 6 profiled gut samples of this project (Sandpiper SingleM profiles, GTDB R232; low-depth and non-metagenome profiles left out).

Field coverage
fieldsamplescoverageR1R2R3R4
age_category18 100%
country18 100%18
health_condition0 0%
subject_id18 100%18
age_at_collection_days18 100%18
Papers (2)
  • Exploring Molecular Interactions between Human Milk Hormone Insulin and Bifidobacteria. PMID 37191543 PMC10269646 curated
  • Compiling an early life human gut microbiome atlas and identification of key microbial drivers. PMID 41350579 PMC12769767 curated
Details
Archive description

16S rRNA sequences of feeding tube segments, residual feeding tube contents, and fecal samples. A small subset of samples also have whole metagenomic sequencing data.

How this project entered the registry

Classified as human gut / stool shotgun DNA metagenome of infant / toddler and neonate by deterministic_prior with confidence 0.90. Assay shotgun_dna.

  • host external_curation.infant_catalog · “infant triage verdict include”
  • body site study.description · “tube contents, and fecal samples. A small”
  • body site external_curation.infant_catalog · “infant triage verdict include (gut/stool scope)”
  • life stage external_curation.infant_catalog · “infant triage verdict include (0-3 y scope)”
  • life stage study.title · “Diversity of NICU Infant Feeding Tubes”
  • life stage study.title · “Microbial Diversity of NICU Infant Feeding Tubes”
Evidence examples (one per field × route)
samplefieldvaluerouteconf.sourcequote
SAMN09985562age_at_collection_days14R10.90biosample_attr14 Days
SAMN09985562countryUSR10.90biosample_attrUSA: Davis
SAMN09985562subject_id35R10.85biosample_attr35
Sequencing

18 runs over 18 samples · mean 3.26 Gbp per run (median 3.33 Gbp, total 58.75 Gbp; 18 runs with a base count) · layout PAIRED 18 · platform ILLUMINA 18 · models Illumina HiSeq 4000 (18) · 94% of runs with a Sandpiper profile · first public 2019-06-15

Authors (18)

Rizzo SM first · Alessandri G · Lugli GA · Fontana F · Tarracchini C · Mancabelli L · Viappiani A · Bianchi MG · Bussolati O · van Sinderen D · Ventura M · Turroni F last · Longhi G · Gennaioli E · Muscò A · Vitale SG · Angioni S · Milani C

Downloads

PRJNA489693.csv.gz (1.4 KB) · parquet (69.5 KB) — all 18 samples, every field with route and confidence · PRJNA489693_determinations.csv.gz (627 B) — 54 evidence rows

Samples (18 · every field this project has values for · hover a cell for its source)
Loading the sample table…