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PRJNA491626 SRP162387

Gut virome in Cameroonian GI-infection patients near bats

Gut virome of Cameroonians living in close proximity with bats

63 samples from Cameroon (Kumba and Lysoka) from patients with GI infection/diarrhoea, studied to identify viral gastroenteritis agents linked to interspecies transmission from bats; cross-sectional design. machine-written summary

63 samples · 63 runs · CM · Age unknown · 2018 · 1.15 Gbp / sample

Sources
✓Sequence archive 378○Abstract / description○Full text○Supplementary tables✓External resources 126–User contribution–Human verified

Checked this project's papers and archive records yourself? Mark it human-verified.

what each source gave
✓ Sequence archive378 sample values (collection date, country, latitude, location locality, location region, longitude)
○ Abstract / descriptionread; no usable statement
○ Full textread; no usable statement or table · PMC6344602, PMC8143477, PMC8201803
○ Supplementary tablesfiles read; no table could be matched to the samples
✓ External resources126 sample values (health condition, health condition detail) · from African Human Microbiome Portal (H3ABioNet)
– User contributionnothing uploaded yet · share metadata
– Human verifiednot yet checked by a person · I checked this project — verify it
Samples at a glance
2 sampling locations · 1 country
Age
unknown63
Condition
gi_infection_or_diarrhoea63
Country
CM63
Body site
primary63
Sequencing depth per sample · this project all projects
all projects, 0–0.03 Gbp: 25,080 samples (4 %)all projects, 0.03–0.1 Gbp: 17,066 samples (3 %)all projects, 0.1–0.3 Gbp: 29,179 samples (5 %)all projects, 0.3–1 Gbp: 47,044 samples (8 %)this project, 0.3–1 Gbp: 16 samplesall projects, 1–3 Gbp: 124,983 samples (22 %)this project, 1–3 Gbp: 45 samplesall projects, 3–10 Gbp: 253,728 samples (44 %)this project, 3–10 Gbp: 2 samplesall projects, 10–30 Gbp: 72,533 samples (13 %)all projects, 30–100 Gbp: 3,024 samples (1 %)all projects, 100–300 Gbp: 106 samples (0 %)all projects, ≥ 300 Gbp: 9 samples (0 %)project median 1.15 Gbp per sample0130Gbp
Community composition
Prevotella 29.3%Faecalibacterium 7.6%Ruminococcoides 4.0%Alloprevotella 2.2%Ruminococcus_C 1.9%Ureaplasma 1.7%Turicibacter 1.6%Gemmiger 1.4%UMGS1491 1.3%CAG-475 1.0%Enterocloster 0.9%Fimenecus 0.8%not assigned to a genus 34.6%other genera 11.7%

Genera, mean relative abundance over 54 profiled gut samples of this project (Sandpiper SingleM profiles, GTDB R232; low-depth and non-metagenome profiles left out).

Field coverage
fieldsamplescoverageR1R2R3R4
age_category0 0%
country63 100%63
health_condition63 100%63
subject_id0 0%
collection_date63 100%63
detailed_location63 100%
health_condition_detail63 100%63
latitude63 100%63
location_locality63 100%63
location_region63 100%63
longitude63 100%63
Papers (3)
  • A catalog of tens of thousands of viruses from human metagenomes reveals hidden associations with chronic diseases. 2021 PMID 34083435 PMC8201803 own_data
  • Gut Virome Analysis of Cameroonians Reveals High Diversity of Enteric Viruses, Including Potential Interspecies Transmitted Viruses. 2019 PMID 30674646 PMC6344602 own_data
  • Novel Siphoviridae Bacteriophages Infecting Bacteroides uniformis Contain Diversity Generating Retroelement. 2021 PMID 33919474 PMC8143477 unsure
Details
Archive description

To identify viral gastroenteritis aetiological agents in Cameroonian patients arising as a result of interspecies transmission from bats.

How this project entered the registry

Classified as human gut / stool shotgun DNA metagenome of age unknown by sonnet_x2 with confidence 0.80. Assay shotgun_dna.

  • host run.host_tax_id · “9606”
  • body site study.title · “Gut virome of Cameroonians”
Evidence examples (one per field × route)
samplefieldvaluerouteconf.sourcequote
SAMN10080877collection_date2014R10.90biosample.attribute:collection_date2014
SAMN10080877countryCMR10.90sample.attr.geo_loc_nameCameroon: Kumba
SAMN10080877health_conditiongi_infection_or_diarrhoeaR20.70external.ahmp sourceDISEASE: Diarrhea
SAMN10080877health_condition_detailDiarrheaR20.70external.ahmp sourceDISEASE: Diarrhea
SAMN10080877latitude4.6333R10.90biosample.attribute:lat_lon4.6333 N 9.445 E
SAMN10080877location_localityKumbaR10.85biosample.attribute:geo_loc_nameCameroon: Kumba
SAMN10080877location_regionSouth-West ProvinceR10.60biosample.attribute:lat_lon4.6333 N 9.445 E
SAMN10080877longitude9.4450R10.90biosample.attribute:lat_lon4.6333 N 9.445 E
Sequencing

63 runs over 63 samples · mean 1.37 Gbp per run (median 1.15 Gbp, total 86.16 Gbp; 63 runs with a base count) · layout PAIRED 63 · platform ILLUMINA 63 · models NextSeq 500 (63) · 89% of runs with a Sandpiper profile · first public 2018-09-24

Authors (27)

Yinda CK first · Vanhulle E · Conceição-Neto N · Beller L · Deboutte W · Shi C · Ghogomu SM · Maes P · Van Ranst M · Matthijnssens J last · Hedžet S first · Rupnik M · Accetto T last · Tisza MJ first · Buck CB last · Zeng S first · Almeida A · Li S · Ying J · Wang H · Qu Y · Paul Ross R · Stanton C · Zhou Z · Niu X · Mu D · Wang S last

Help complete this project

2 of the 4 core fields are below 50% coverage: age_category, subject_id. Share metadata

Downloads

PRJNA491626.csv.gz (2.4 KB) · parquet (72.0 KB) — all 63 samples, every field with route and confidence · PRJNA491626_determinations.csv.gz (4.2 KB) — 504 evidence rows

Samples (63 · every field this project has values for · hover a cell for its source)
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