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PRJNA527369 SRP188584

IBD fecal metagenomes, Vercelli, Italy, cross-sectional

Whole-metagenome shotgun sequencing of 13 IBD Italian fecal metagenomes

13 fecal metagenome samples from individuals with IBD in Vercelli, Italy, cross-sectional design, used as a disease-specificity control for CRC biomarkers in a larger study. machine-written summary

13 samples · 13 runs · IT · Age unknown · 2019 · 4.08 Gbp / sample

Sources
✓Sequence archive 78✓Abstract / description 26–Full text–Supplementary tables–External resources–User contribution–Human verified

Checked this project's papers and archive records yourself? Mark it human-verified.

what each source gave
✓ Sequence archive78 sample values (collection date, country, latitude, location locality, location region, longitude)
✓ Abstract / description26 sample values (health condition, health condition detail)
– Full textno open-access full text linked
– Supplementary tablesno supplementary tables available (or none open access)
– External resourcesnot covered by the external resources we ingest
– User contributionnothing uploaded yet
– Human verifiednot yet checked by a person · I checked this project — verify it
Samples at a glance
1 sampling locations · 1 country
Age
unknown13
Condition
ibd_unspecified13
Country
IT13
Body site
primary13
Sequencing depth per sample · this project all projects
all projects, 0–0.03 Gbp: 25,080 samples (4 %)all projects, 0.03–0.1 Gbp: 17,066 samples (3 %)all projects, 0.1–0.3 Gbp: 29,179 samples (5 %)all projects, 0.3–1 Gbp: 47,044 samples (8 %)all projects, 1–3 Gbp: 124,983 samples (22 %)this project, 1–3 Gbp: 2 samplesall projects, 3–10 Gbp: 253,728 samples (44 %)this project, 3–10 Gbp: 11 samplesall projects, 10–30 Gbp: 72,533 samples (13 %)all projects, 30–100 Gbp: 3,024 samples (1 %)all projects, 100–300 Gbp: 106 samples (0 %)all projects, ≥ 300 Gbp: 9 samples (0 %)project median 4.08 Gbp per sample0130Gbp
Community composition
Phocaeicola 14.8%Prevotella 14.4%Bacteroides 11.9%Gemmiger 4.0%Alistipes 4.0%Faecalibacterium 3.6%Klebsiella 3.4%Escherichia 3.3%Bifidobacterium 2.4%Megasphaera 2.2%Dialister 2.0%Sutterella 1.8%not assigned to a genus 6.3%other genera 25.9%

Genera, mean relative abundance over 13 profiled gut samples of this project (Sandpiper SingleM profiles, GTDB R232; low-depth and non-metagenome profiles left out).

Field coverage
fieldsamplescoverageR1R2R3R4
age_category0 0%
country13 100%13
health_condition13 100%13
subject_id0 0%
collection_date13 100%13
detailed_location13 100%
health_condition_detail13 100%13
latitude13 100%13
location_locality13 100%13
location_region13 100%13
longitude13 100%13
Details
Archive description

In the context of a larger whole-genome-shotgun metagenomic study about colorectal cancer (CRC), this set of samples served as an integration of the final validation of the discovered biomarkers: the rationale behind is collecting a moiety of samples related to a different disease with respect to the condition which constitute the main focus of the study, in order to use them to test the specificity of the biomarkers towards CRC.

How this project entered the registry

Classified as human gut / stool shotgun DNA metagenome of age unknown by sonnet_x2 with confidence 0.75. Assay shotgun_dna.

  • host run.scientific_name · “human gut metagenome”
  • body site study.title · “Italian fecal metagenomes”
Evidence examples (one per field × route)
samplefieldvaluerouteconf.sourcequote
SAMN11153477collection_date2016-01-11R10.90biosample.attribute:collection_date2016-01-11
SAMN11153477countryITR10.90sample.attr.geo_loc_nameItaly: Vercelli
SAMN11153477health_conditionibd_unspecifiedR40.50study.titleIBD Italian fecal metagenomes
SAMN11153477health_condition_detailIBD (unspecified type); used as validation cohort for CRC biomarkersR40.50study.titleIBD Italian fecal metagenomes
SAMN11153477latitude45.4140R10.90biosample.attribute:lat_lon45.414028 N 9.208268 E
SAMN11153477location_localityVercelliR10.85biosample.attribute:geo_loc_nameItaly: Vercelli
SAMN11153477location_regionLombardyR10.60biosample.attribute:lat_lon45.414028 N 9.208268 E
SAMN11153477longitude9.2083R10.90biosample.attribute:lat_lon45.414028 N 9.208268 E
Sequencing

13 runs over 13 samples · mean 4.69 Gbp per run (median 4.08 Gbp, total 61.02 Gbp; 13 runs with a base count) · layout PAIRED 13 · platform ILLUMINA 13 · models Illumina HiSeq 2000 (13) · 100% of runs with a Sandpiper profile · first public 2019-11-15

Authors (0)

No author record.

Downloads

PRJNA527369.csv.gz (1.3 KB) · parquet (70.5 KB) — all 13 samples, every field with route and confidence · PRJNA527369_determinations.csv.gz (1.2 KB) — 104 evidence rows

Samples (13 · every field this project has values for · hover a cell for its source)
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