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PRJNA529124 SRP189572

Gut metagenomes of healthy individuals from Ghana

Metagenomic sequencing of stool samples from Ghanaian individuals

44 stool metagenome samples from healthy individuals in the Asante Akim North district, Ghana, spanning age groups from neonates to adults, sequenced on Illumina HiSeq 2500. machine-written summary

44 samples · 44 runs · GH · Infant / toddler · 2019 · 4.85 Gbp / sample

Sources
✓Sequence archive 44✓Abstract / description 44○Full text✓Supplementary tables 27✓External resources 131–User contribution–Human verified

Checked this project's papers and archive records yourself? Mark it human-verified.

what each source gave
✓ Sequence archive44 sample values (country)
✓ Abstract / description44 sample values (location locality)
○ Full textread; no usable statement or table · PMC10234599, PMC10902438, PMC11019788
✓ Supplementary tables27 sample values (subject id)
✓ External resources131 sample values (age, health condition, health condition detail) · from curatedMetagenomicData 3 (cMD 3)
– User contributionnothing uploaded yet
– Human verifiednot yet checked by a person · I checked this project — verify it
Samples at a glance
1 country
Age
adult22
child14
adolescent3
infant3
neonate1
unknown1
Condition
healthy_control44
Country
GH44
Body site
primary44
Sequencing depth per sample · this project all projects
all projects, 0–0.03 Gbp: 25,080 samples (4 %)all projects, 0.03–0.1 Gbp: 17,066 samples (3 %)all projects, 0.1–0.3 Gbp: 29,179 samples (5 %)all projects, 0.3–1 Gbp: 47,044 samples (8 %)all projects, 1–3 Gbp: 124,983 samples (22 %)this project, 1–3 Gbp: 4 samplesall projects, 3–10 Gbp: 253,728 samples (44 %)this project, 3–10 Gbp: 39 samplesall projects, 10–30 Gbp: 72,533 samples (13 %)this project, 10–30 Gbp: 1 samplesall projects, 30–100 Gbp: 3,024 samples (1 %)all projects, 100–300 Gbp: 106 samples (0 %)all projects, ≥ 300 Gbp: 9 samples (0 %)project median 4.85 Gbp per sample0130Gbp
Community composition
Prevotella 30.2%Faecalibacterium 5.0%Roseburia 3.8%Bacteroides 3.5%Vescimonas 2.9%Phocaeicola 2.7%Acetatifactor 2.5%CAG-177 1.9%Ruminococcoides 1.9%Faecousia 1.8%Succinivibrio 1.6%Dialister 1.6%not assigned to a genus 5.7%other genera 34.8%

Genera, mean relative abundance over 41 profiled gut samples of this project (Sandpiper SingleM profiles, GTDB R232; low-depth and non-metagenome profiles left out).

Field coverage
fieldsamplescoverageR1R2R3R4
age_category43 98%
country44 100%44
health_condition44 100%44
subject_id27 61%27
detailed_location44 100%
health_condition_detail44 100%44
location_locality44 100%44
age_at_collection_days43 98%43
Papers (8)
  • (no title) curated
  • (no title) curated
  • Maternal and food microbial sources shape the infant microbiome of a rural Ethiopian population. PMID 37116481 PMC10234599 curated
  • (no title) curated
  • Genomic and ecological approaches to identify the Bifidobacterium breve prototype of the healthy human gut microbiota. PMID 38426063 PMC10902438 curated
3 more
  • Taxonomic and metabolic development of the human gut microbiome across life stages: a worldwide metagenomic investigation. PMID 38441032 PMC11019788
  • (no title)
  • (no title)
Details
Archive description

Stool samples of a cohort of individuals from Ghana (Asante Akim North district). Samples were sequenced with Illumina HiSeq 2500 using NexteraXT library preparation.

How this project entered the registry

Classified as human gut / stool shotgun DNA metagenome of infant / toddler by deterministic_prior with confidence 0.90. Assay shotgun_dna.

  • host external_curation.infant_catalog · “infant triage verdict include”
  • body site study.title · “Metagenomic sequencing of stool samples from Ghanaian”
  • body site study.description · “Stool samples of a”
  • life stage external_curation.infant_catalog · “infant triage verdict include (0-3 y scope)”
Evidence examples (one per field × route)
samplefieldvaluerouteconf.sourcequote
SAMN11265215age_at_collection_days12418.5R20.75external.cmd sourceage: 34; age_unit: Year; age_group: Adult
SAMN11265215countryGHR10.90biosample_attrGhana
SAMN11265215health_conditionhealthy_controlR20.75external.cmd sourcedisease: Healthy
SAMN11265215health_condition_detailHealthyR20.75external.cmd sourcedisease: Healthy
SAMN11265215location_localityAsante Akim North districtR40.45ena.study_descriptionGhana (Asante Akim North district)
SAMN11265215subject_idC16-10022-GH_MR20.80paper.supp.mmc7.xlsx[A!subjectID]sample=C16-20005-GH; subjectID=C16-10022-GH_M
Sequencing

44 runs over 44 samples · mean 4.97 Gbp per run (median 4.85 Gbp, total 218.81 Gbp; 44 runs with a base count) · layout PAIRED 44 · platform ILLUMINA 44 · models Illumina HiSeq 2000 (44) · 93% of runs with a Sandpiper profile · first public 2019-03-28

Authors (146)

Tett A first · Huang KD · Asnicar F · Fehlner-Peach H · Pasolli E · Karcher N · Armanini F · Manghi P · Bonham K · Zolfo M · De Filippis F · Magnabosco C · Bonneau R · Lusingu J · Amuasi J · Reinhard K · Rattei T · Boulund F · Engstrand L · Zink A · Collado MC · Littman DR · Eibach D · Ercolini D · Rota-Stabelli O · Huttenhower C · Maixner F · Segata N last · Tomofuji Y first · Kishikawa T · Maeda Y · Ogawa K · Otake-Kasamoto Y · Kawabata S · Nii T · Okuno T · Oguro-Igashira E · Kinoshita M · Takagaki M · Oyama N · Todo K · Yamamoto K · Sonehara K · Yagita M · Hosokawa A · Motooka D · Matsumoto Y · Matsuoka H · Yoshimura M · Ohshima S · Shinzaki S · Nakamura S · Iijima H · Inohara H · Kishima H · Takehara T · Mochizuki H · Takeda K · Kumanogoh A · Okada Y last · Manara S first · Selma-Royo M · Blanco-Miguez A · Cumbo F · Golzato D · Pinto F · Valles-Colomer M · Amoroso L · Corrias MV · Ponzoni M · Raffaetà R · Cabrera-Rubio R · Olcina M · Zhu J first · Xie H · Yang Z · Chen J · Yin J · Tian P · Wang H · Zhao J · Zhang H · Lu W · Chen W last · Argentini C first · Lugli GA · Tarracchini C · Fontana F · Mancabelli L · Viappiani A · Anzalone R · Angelini L · Alessandri G · Longhi G · Bianchi MG · Taurino G · Bussolati O · Milani C · van Sinderen D · Turroni F · Ventura M last · De Biase R · Bocchio F · De Conto F · Nouvenne A · Ticinesi A · Meschi T · Cecchi R · Lin NY first · Fukuoka S · Koyama S · Tourlousse DM · Shigeno Y · Yamano H · Murotomi K · Tamaki H · Irie T · Sugiyama E · Kumagai S · Itahashi K · Tanegashima T · Fujimaki K · Ito S · Shindo M · Tsuji T · Wake H · Watanabe K · Enokida T · Tahara M · Yamashita R · Fujisawa T · Nomura M · Kawazoe A · Goto K · Doi T · Shitara K · Mano H · Sekiguchi Y · Benno Y · Nishikawa H last · Sola L first · Candeliere F · Busi E · Raimondi S · Amaretti A · Rossi M last

Downloads

PRJNA529124.csv.gz (2.4 KB) · parquet (71.8 KB) — all 44 samples, every field with route and confidence · PRJNA529124_determinations.csv.gz (3.5 KB) — 246 evidence rows

Samples (44 · every field this project has values for · hover a cell for its source)
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