FMT in Multiple Sclerosis: n-of-1 longitudinal study (USA)
7 fecal metagenome samples from an adult RRMS subject in the USA, in an n-of-1 longitudinal study evaluating fecal microbiota transplantation (FMT) effects on the gut microbiome. machine-written summary
7 samples · 7 runs · US · Adult · 2019 · 1.20 Gbp / sample
Checked this project's papers and archive records yourself? Mark it human-verified.
what each source gave
| ✓ Sequence archive | 28 sample values (collection date, country, intervention, subject id, timepoint) |
| ✓ Abstract / description | 14 sample values (health condition, health condition detail) |
| ○ Full text | read; no usable statement or table · PMC7506051 |
| ○ Supplementary tables | files read; no table could be matched to the samples |
| – External resources | not covered by the external resources we ingest |
| – User contribution | nothing uploaded yet |
| – Human verified | not yet checked by a person · I checked this project — verify it |
intervention — arms not resolved
Faecal microbiota transplantation design single_arm_before_after · population autoimmune_inflammatory · Fecal microbiota transplantation, single-subject time series
“impact of fecal-microbiota-transplantation (FMT)” — abstract:33013647 (study level; per-sample arms are in the intervention column below)
longitudinalpartners / couples
1 of 2 people sampled more than once (6 samples; median 6, up to 6 per person) · follow-up median 12 mo (max 12 mo) · median interval between samples 3 mo (time from timepoint labels). Schedule stated by the source: “Baseline, 3 weeks, 13 weeks, 26 weeks, 39 weeks, 52 weeks post-baseline for MS subject; single sample from spouse”; stated follow-up 12 mo.
Each row is one person (1 shown); dots are samples at days since that person's first sample.
The MS subject's spouse (household partner) donated one stool sample as a control; also FMT donor-recipient relationship exists between subject and FMT donors (though donors not sampled). 6 of 7 sampl.
partner · circles are people (pale = no sequenced sample), arrows run donor → recipient and parent → child, curved lines join twins, partners and siblings, squares are shared settings or families without recorded roles. Shown: 1 linked group from explicit relations. 2 people in the project overall.
Genera, mean relative abundance over 7 profiled gut samples of this project (Sandpiper SingleM profiles, GTDB R232; low-depth and non-metagenome profiles left out).
| field | samples | coverage | R1 | R2 | R3 | R4 |
|---|---|---|---|---|---|---|
| age_category | 7 | 100% | ||||
| country | 7 | 100% | 7 | |||
| health_condition | 7 | 100% | 7 | |||
| subject_id | 7 | 100% | 7 | |||
| collection_date | 7 | 100% | 7 | |||
| health_condition_detail | 7 | 100% | 7 | |||
| timepoint_label | 6 | 86% | 6 | |||
| intervention | 1 | 14% | 1 |
- Single-Arm, Non-randomized, Time Series, Single-Subject Study of Fecal Microbiota Transplantation in Multiple Sclerosis. 2020 PMID 33013647 PMC7506051 own_data
Archive description
This would be the first single case / n-of-1 report of a fecal microbiota transplantation (FMT) in a Multiple Sclerosis (MS) subject depicting shotgun metagenomics sequencing.
How this project entered the registry
Classified as human gut / stool shotgun DNA metagenome of adult by deterministic_prior with confidence 0.90. Assay shotgun_dna.
- host external_curation.infant_catalog · “infant triage reason_code age_adult_only”
- body site study.title · “of 1 study: Fecal Microbiota Transplantation in”
- body site study.description · “report of a fecal microbiota transplantation (FMT)”
- life stage external_curation.infant_catalog · “infant triage reason_code age_adult_only”
Evidence examples (one per field × route)
| sample | field | value | route | conf. | source | quote |
|---|---|---|---|---|---|---|
| SAMN11259824 | collection_date | 2018 | R1 | 0.90 | biosample.attribute:collection_date | 2018 |
| SAMN11259824 | country | US | R1 | 0.90 | sample.attr.geo_loc_name | USA |
| SAMN11259824 | health_condition | neurological_psychiatric | R4 | 0.50 | paper.abstract | Relapsing-Remitting-Multiple-Sclerosis (RRMS) |
| SAMN11259824 | health_condition_detail | Single subject with RRMS | R4 | 0.50 | paper.abstract | Relapsing-Remitting-Multiple-Sclerosis (RRMS) |
| SAMN11259824 | intervention | no_intervention | R1 | 0.85 | biosample.attribute:host_subject_id | Spouse |
| SAMN11259824 | subject_id | Spouse | R1 | 0.85 | sample.attr.host_subject_id | Spouse |
| SAMN11259825 | timepoint_label | Baseline | R1 | 0.70 | sample.name_rule:description | Baseline |
Sequencing
7 runs over 7 samples · mean 1.25 Gbp per run (median 1.20 Gbp, total 8.75 Gbp; 7 runs with a base count) · layout PAIRED 7 · platform ILLUMINA 7 · models NextSeq 500 (7) · 100% of runs with a Sandpiper profile · first public 2019-03-29
Authors (18)
Engen PA first · Zaferiou A · Rasmussen H · Naqib A · Green SJ · Fogg LF · Forsyth CB · Raeisi S · Hamaker B · Keshavarzian A last · Tarracchini C first · Fontana F · Mancabelli L · Lugli GA · Alessandri G · Turroni F · Ventura M · Milani C last
Downloads
PRJNA529230.csv.gz (1.1 KB) · parquet (70.0 KB) — all 7 samples, every field with route and confidence · PRJNA529230_determinations.csv.gz (986 B) — 42 evidence rows