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PRJNA529400 SRP189832

Gut Metagenome of Healthy Cohort in Korogwe, Tanzania

Homo sapiens Metagenome

Longitudinal cohort study of 68 stool samples from healthy individuals across age groups (infants to adults) in Korogwe District, Tanzania, sequenced by Illumina HiSeq 2500 with Nextera XT library preparation. machine-written summary

68 samples · 68 runs · TZ · Infant / toddler · 2019 · 4.93 Gbp / sample

Sources
✓Sequence archive 68✓Abstract / description 68○Full text○Supplementary tables✓External resources 204–User contribution–Human verified

Checked this project's papers and archive records yourself? Mark it human-verified.

what each source gave
✓ Sequence archive68 sample values (country)
✓ Abstract / description68 sample values (location region)
○ Full textread; no usable statement or table · PMC10234599
○ Supplementary tablesfiles read; no table could be matched to the samples
✓ External resources204 sample values (age, health condition, health condition detail) · from curatedMetagenomicData 3 (cMD 3)
– User contributionnothing uploaded yet
– Human verifiednot yet checked by a person · I checked this project — verify it
Samples at a glance
1 country
Age
adult36
child14
adolescent9
infant6
unknown2
neonate1
Condition
healthy_control68
Country
TZ68
Body site
unknown68
Sequencing depth per sample · this project all projects
all projects, 0–0.03 Gbp: 25,080 samples (4 %)all projects, 0.03–0.1 Gbp: 17,066 samples (3 %)all projects, 0.1–0.3 Gbp: 29,179 samples (5 %)all projects, 0.3–1 Gbp: 47,044 samples (8 %)this project, 0.3–1 Gbp: 1 samplesall projects, 1–3 Gbp: 124,983 samples (22 %)this project, 1–3 Gbp: 4 samplesall projects, 3–10 Gbp: 253,728 samples (44 %)this project, 3–10 Gbp: 62 samplesall projects, 10–30 Gbp: 72,533 samples (13 %)this project, 10–30 Gbp: 1 samplesall projects, 30–100 Gbp: 3,024 samples (1 %)all projects, 100–300 Gbp: 106 samples (0 %)all projects, ≥ 300 Gbp: 9 samples (0 %)project median 4.93 Gbp per sample0130Gbp
Community composition
Prevotella 33.2%Succinivibrio 3.3%Roseburia 2.7%Bacteroides 2.5%Faecalibacterium 2.4%Alloprevotella 2.2%Vescimonas 2.1%Cryptobacteroides 2.1%Phocaeicola 1.9%Ruminococcoides 1.8%Acetatifactor 1.7%Treponema_D 1.4%not assigned to a genus 5.7%other genera 37.1%

Genera, mean relative abundance over 58 profiled gut samples of this project (Sandpiper SingleM profiles, GTDB R232; low-depth and non-metagenome profiles left out).

Field coverage
fieldsamplescoverageR1R2R3R4
age_category66 97%
country68 100%68
health_condition68 100%68
subject_id0 0%
age_at_collection_days68 100%68
detailed_location68 100%
health_condition_detail68 100%68
location_region68 100%68
Papers (1)
  • Maternal and food microbial sources shape the infant microbiome of a rural Ethiopian population. PMID 37116481 PMC10234599 curated
Details
Archive description

Stool samples of a cohort of individuals from Tanzania (Korogwe District). Samples weresequenced with Illumina HiSeq 2500 using NexteraXT library preparation.

How this project entered the registry

Classified as human gut / stool shotgun DNA metagenome of infant / toddler by deterministic_prior with confidence 0.90. Assay shotgun_dna.

  • host external_curation.infant_catalog · “infant triage verdict include”
  • body site study.description · “Stool samples of a”
  • body site external_curation.infant_catalog · “infant triage verdict include (gut/stool scope)”
  • life stage external_curation.infant_catalog · “infant triage verdict include (0-3 y scope)”
Evidence examples (one per field × route)
samplefieldvaluerouteconf.sourcequote
SAMN11283148age_at_collection_days8766.0R20.75external.cmd sourceage: 24; age_unit: Year; age_group: Adult
SAMN11283148countryTZR10.90biosample_attrTanzania
SAMN11283148health_conditionhealthy_controlR20.75external.cmd sourcedisease: Healthy
SAMN11283148health_condition_detailHealthyR20.75external.cmd sourcedisease: Healthy
SAMN11283148location_regionKorogwe DistrictR40.45ena.study_descriptionindividuals from Tanzania (Korogwe District)
Sequencing

68 runs over 68 samples · mean 5.01 Gbp per run (median 4.93 Gbp, total 340.96 Gbp; 68 runs with a base count) · layout PAIRED 68 · platform ILLUMINA 68 · models Illumina HiSeq 2500 (68) · 85% of runs with a Sandpiper profile · first public 2019-03-30

Authors (20)

Manara S first · Selma-Royo M · Huang KD · Asnicar F · Armanini F · Blanco-Miguez A · Cumbo F · Golzato D · Manghi P · Pinto F · Valles-Colomer M · Amoroso L · Corrias MV · Ponzoni M · Raffaetà R · Cabrera-Rubio R · Olcina M · Pasolli E · Collado MC · Segata N last

Downloads

PRJNA529400.csv.gz (2.9 KB) · parquet (72.8 KB) — all 68 samples, every field with route and confidence · PRJNA529400_determinations.csv.gz (3.8 KB) — 340 evidence rows

Samples (68 · every field this project has values for · hover a cell for its source)
Loading the sample table…